Molecular epidemiology of rabies virus in Romania provides evidence for a high degree of heterogeneity and virus diversity

Turcitu, Mihai; Bărboi, G.; Vuta, Vlad; Mihai, Iuliana Oana; Boncea, D.; Dumitrescu, Florina; Codreanu, Mario; Johnson, Nicholas; Fooks, Anthony R.; Müller, Thomas; Freuling, Conrad Martin

doi:10.1016/j.virusres.2010.02.008

Cited by 23 publications

(35 citation statements)

References 14 publications

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“…One reason for the low genetic diversity of currently circulating RABV strains in the west Balkans and Croatia could be natural barriers like rivers or high mountain ranges which may play a significant role in limiting the spread of distinct rabies groups (Bourhy and others 1999, Johnson and others 2007, McElhinney and others 2011). However, it is known that Romanian sequences showed a high degree of heterogeneity with six different lineages identified and that the RABV group most similar to the NEE group was isolated in Romania's northern regions where the country borders with Ukraine (Turcitu and others 2010). Another very recent study characterised Ukrainian sequences as NEE or Russian group C (Picard-Meyer and others 2012).…”

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confidence: 99%

Clinical rabies in cattle imported into Croatia

Lojkić¹,

Bedeković

Čač

et al. 2013

Veterinary Record

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confidence: 99%

Clinical rabies in cattle imported into Croatia

Lojkić¹,

Bedeković

Čač

et al. 2013

Veterinary Record

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“…These results correspond to the geographical distribution of rabies variants in Europe. The NEE group is found in western Russia, Finland, Ukraine, Estonia, Lithuania, Latvia, Romania and Slovakia [3, 16, 21, 26, 31], while the CE group is found in eastern Germany, the Czech Republic, and in Slovenia [3, 16]. The Vistula River, which divides the territory of Poland into two parts, might be a natural border in the distribution of RV variants (NEE and CE), as was previously suggested by Bourhy et al [3].…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology of rabies virus in Poland

Orłowska

Zmudzinski

2014

Arch Virol

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The paper describes a phylogenetic study of 58 Polish isolates of rabies virus collected between 1992 and 2010. Sequences of the nucleoprotein (N) and glycoprotein (G) genes approximately 600 bp long were compared with reference sequences (GenBank) of European rabies viruses from neighbouring countries. The study confirmed a very high level of homology (94.4–100 %) of the Polish rabies virus strains irrespective of the date of isolation. Two variants of rabies virus: NEE (Northeastern Europe variant) and CE (Central Europe variant), depending on the geographical place of isolation, were circulating in Poland from 1992 to 2010. The Polish rabies virus isolates showed high similarity to European RABV strains, especially those collected in Ukraine and Romania. They were clearly different from vaccine strains SAD B19 and SAD Bern, which have been used for oral vaccination of foxes against rabies in Poland since 1993.

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Section: Introductionmentioning

confidence: 99%

“…Thus, the occurrence of RV could be restricted to the Balkan Peninsula and the eastern European plain. Although ORV holds the promise that sylvatic rabies might be eliminated in the future, the large geographic areas where rabies is still enzootic and the occasional reintroductions of RV in rabies-free areas justify the need for continuous surveillance and control until eradication programmes succeed (Mulatti, Ferr e, Patregnani, Bonfanti, & Marangon, 2011;Turcitu et al, 2010;Picard-Meyer et al, 2012). [WE]), one each associated with a fairly well-defined geographic distribution (Bourhy et al, 1999) based on the comparisons of the nucleotide sequence of N gene, that is the most conserved gene of the rabies genome and the most abundantly represented genomic region of RV in GenBank.…”

Section: Introductionmentioning

confidence: 99%

“…This subspecies classification scheme was further improved by Kuzmin et al (2004) and then by McElhinney et al (2006McElhinney et al ( , 2011. Molecular genetic analysis of circulating RV strains has been proven to be a useful tool when efforts to track the RV spread or to uncover the origin of RV strains in an area where the elimination programme minimized the occurrence of RV are made (Orłowska & Zmudzinski, 2014;Turcitu et al, 2010;Picard-Meyer et al, 2012;Rihtari c, Hostnik, Grom, & Toplak, 2011;Fusaro et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Resurgence of rabies in Hungary during 2013-2014: An attempt to track the origin of identified strains

Hornyák

Juhász

Forró

et al. 2017

Transbound Emerg Dis

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SummaryIn 2013-2014, accumulation of rabies episodes raised concerns regarding ongoing elimination programme in Hungary. Nearly four dozen cases were identified over a 13-month period in the central region of the country far behind the immunization zones. Although the outbreak was successfully controlled, the origin of disease remained unknown. In this study, we sequenced the partial N and G genes from 47Hungarian rabies virus (RV) strains isolated from the 2013-2014 outbreak. Sequencing and phylogenetic analysis of the N and G genes showed that the Hungarian RV isolates share high nucleotide similarity among each other (up to 100%). When analysing the N gene, comparable sequence similarity was seen between the outbreak strains and some historic Romanian RV strains. Unfortunately, in the lack of available sequence data from the Romanian RV strains, the genetic relationship within the G gene could not be determined. Phylogenetic analysis of Hungarian RV isolates detected in the past revealed that multiple independent RV lineages circulated in our country over the past 25 years. The parental strain of the 2013-2014 outbreak may have been imported independently perhaps from east through transborder movement of a reservoir animal. Next to the introduction, this imported RV strain seems to have spread clonally in the affected area. Our findings indicate that despite effective control measures that, overall, minimized the incidence of rabies over the past decade, field and laboratory monitoring needs to be continued to make rabies elimination programme in Hungary successful.

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Molecular epidemiology of rabies virus in Romania provides evidence for a high degree of heterogeneity and virus diversity

Cited by 23 publications

References 14 publications

Clinical rabies in cattle imported into Croatia

Clinical rabies in cattle imported into Croatia

Molecular epidemiology of rabies virus in Poland

Resurgence of rabies in Hungary during 2013-2014: An attempt to track the origin of identified strains

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