Molecular epidemiology of rabies virus strains isolated from wild canids in Northeastern Brazil

Carnieli, Pedro; Brandão, Paulo Eduardo; Carrieri, Maria Luisa; Castilho, Juliana Galera; Macedo, Carla Isabel; Machado, Lindenberg M.; Rangel, Normélia; Carvalho, Rosangela Cavalcanti de; Carvalho, Vania Alves de; Montebello, Lúcia; Wada, Marcelo Yoshito; Kotait, Ivanete

doi:10.1016/j.virusres.2006.02.007

Cited by 48 publications

(45 citation statements)

References 27 publications

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“…Fox RV isolates from northeastern Brazil have been reported to form specific clades that group independently of dog RV isolates despite evidence suggesting that dog and fox rabies are derived from a common ancestor [2,4,22,23]. In this study, although lineage A consisted primarily fox RV isolates, several RV isolates from sublineage A-1, which consisted of dog RV isolates, were placed within sublineages of A-2 and A-3, which consisted of fox RV isolates.…”

Section: Discussionmentioning

confidence: 68%

“…In this study, although lineage A consisted primarily fox RV isolates, several RV isolates from sublineage A-1, which consisted of dog RV isolates, were placed within sublineages of A-2 and A-3, which consisted of fox RV isolates. One dog infected with fox-typed RV was found in the state of Pernambuco in northeastern Brazil [4]. While not conclusively demonstrated, it is postulated that the dog RV isolates of sublineage A-1 may have been directly or indirectly transmitted by rabid foxes, and the virus might have been spread in the dog population of northeastern regions in the past.…”

Section: Discussionmentioning

confidence: 99%

“…Although the incidence of canine rabies has tended to decrease in response to effective vaccination programs undertaken in urban areas [1], during the period of 1993 to 2002, the highest numbers of canine and human rabies cases transmitted by dogs in the Pan-American region were recorded in Brazil [1]. Recently, new RV variants exhibiting genetic characteristics similar to canine rabies have been isolated from foxes in northeastern Brazil [2,4,23]. Additionally, RVs isolated from dogs in the state of Maranhão were found to consist of 2 distinct phylogenetic groups, with 1 group being genetically similar to the fox RV variant [22].…”

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confidence: 99%

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Phylogenetic Characterization of Rabies Virus Isolates from Carnivora in Brazil

Kobayashi

Inoue

Sato

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. The incidence of canine rabies has been widely reported in Brazil, and new rabies virus (RV) variants, genetically similar to canine RV, have recently been isolated from foxes. In order to derive the epidemiological characteristics of Brazilian Carnivora RV, Brazilian RVs isolated from dogs, cats, and foxes were genetically analyzed. Brazilian Carnivora RV isolates were divided into 2 main lineages. The predominant lineage was found in dogs and cats, which included the Argentinean and Bolivian Carnivora RV isolates, and was extensively distributed throughout Brazil and surrounding countries. The other lineage consisted of three sublineages containing Brazilian dog and fox RV isolates, with the dog sublineages located on an internal branch of 2 fox sublineages, suggesting that RV transmission events might have occurred between foxes and dogs in the past. These results suggest that contact between dogs and wildlife has the potential to generate new rabies variants and that it is important to control RV infection cycles in both dogs and wildlife to prevent spread of rabies infection.

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Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Phylogenetic Characterization of Rabies Virus Isolates from Carnivora in Brazil

Kobayashi

Inoue

Sato

et al. 2007

The Journal of Veterinary Medical Science

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“…There are two distinct variants that circulate in Northeast Brazil and that have as reservoirs Cerdocyon thous (crabeating fox) and Callithrix jacchus (common marmoset). These variants are not compatible with those defined by the CDC panel, but they have a consistent antigenic profile [18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 78%

“…However, antigenic analysis is unable to identify RABV isolates obtained from several reservoirs species because these isolates produced atypical reaction patterns or incompatible profiles. [17][18][19][20][21][22]42]. The genetic analysis of the viral nucleoprotein sequence allowed further characterization of those isolates.…”

Section: Discussionmentioning

confidence: 99%

Monoclonal antibodies for characterization of rabies virus isolated from non-hematophagous bats in Brazil

Chaves¹,

Achkar²,

Silva³

et al. 2015

J Infect Dev Ctries

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Introduction: In Brazil, various isolates of rabies virus (RABV) show antigenic profiles distinct from those established by the reduced panel of eight monoclonal antibodies (MAbs) determined by the Centers for Disease Control and Prevention (CDC), utilized for the antigenic characterization of RABV in the Americas. The objective of this study was to produce MAbs from RABV isolates from insectivorous bats with an antigenic profile incompatible with the pre-established one. Methodology: An isolate of RABV from the species Eptesicus furinalis that showed an antigenic profile incompatible with the panel utilized was selected. Hybridomas were produced utilizing the popliteal lymph nodes of mice immunized with ribonucleoproteins purified from the isolate. Results: Two MAbs-producing clones were obtained, BR/IP1-3A7 and BR/IP2-4E10. Fifty-seven isolates of RABV from different species of animals and different regions of Brazil were analyzed utilizing the MAbs obtained. In the analysis of 23 RABV isolates from nonhematophagous bats, the MAbs cross-reacted with ten isolates, of which four were of the species Nyctinomops laticaudatus, one of the species Eptesicus furinalis, and five of the genus Artibeus. Of the nine isolates of non-hematophagous isolates that displayed an incompatible profile analyzed, characteristic of insectivorous bats, BR/IP1-3A7 reacted with five (55.55%) and BR/IP2-4E10 with four (44.44%). Conclusions: The MAbs obtained were able to recognize epitopes common between the three genera, Artibeus, Eptesicus, and Nyctinomops, thereby allowing the antigenic characterization of RABV isolates in Brazil.

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Characterization of a human–mouse chimeric monoclonal antibody targeting rabies virus glycoprotein

Cai

Yang

et al. 2023

Journal of Medical Virology

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At present, the horse or human rabies immunoglobulin (RIG) used for postexposure prevention of human rabies (PEP) has high cost and limited availability. It is strongly encouraged to replace RIG with equivalent or more effective and safer products. Mouse and human monoclonal antibodies have been shown to protect rodents from lethal rabies virus (RABV) attacks. In this study, we reported a human–mouse chimeric monoclonal antibody, 12‐2A12, which showed a strong neutralization potency and a wide breadth against multiple street viruses of RABV in vitro. The antibody binded the viral glycoprotein (G) with nanomolar affinity. The complex structure of 12‐2A12 bound to RABV G revealed that the antibody recognizes an epitope that partially overlaps with the recognition region for the nicotinic acetylcholine receptor (nAChR). The antibody therefore would interfere with the nAChR/G interaction to block the viral receptor binding. In addition, comparison of our complex structure with the G structure in the acidic state reveals a clear steric clash, highlighting that the antibody would further prevent the conformational changes of the viral glycoprotein that are essential for membrane fusion. In light of these functional and structural data, we believe that 12‐2A12 might be developed to be included in an antibody cocktail for potential use in human rabies PEP.

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Molecular epidemiology of rabies virus strains isolated from wild canids in Northeastern Brazil

Cited by 48 publications

References 27 publications

Phylogenetic Characterization of Rabies Virus Isolates from Carnivora in Brazil

Phylogenetic Characterization of Rabies Virus Isolates from Carnivora in Brazil

Monoclonal antibodies for characterization of rabies virus isolated from non-hematophagous bats in Brazil

Characterization of a human–mouse chimeric monoclonal antibody targeting rabies virus glycoprotein

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