Molecular Genetic Identification of Scots Pine and Siberian Larch Populations in Perm Krai Based on Polymorphism of Issr-PCR Markers

Sboeva, Ya.; Vasileva, Yu.; Chertov, Nikita; Pystogova, Nina; Boronnikova, Svetlana; Kalendar, Ruslan; Мартыненко, Н. Н.

doi:10.15372/sjfs20200405

Cited by 4 publications

(4 citation statements)

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Section: Methodsmentioning

confidence: 99%

“…The ISSR primers were designed by Kalendar et al [24], and in this study ISSR primers for P. sylvestris were used [25]. DNA amplification was conducted using a slightly modified protocol [25,26]. A total of 20 primers were tested initially, and five polymorphic primers producing clearly identifiable and repeatable bands were selected for further analyses (Table 1).…”

Section: Methodsmentioning

confidence: 99%

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Genetic Structure, Differentiation and Originality of Pinus sylvestris L. Populations in the East of the East European Plain

Vasilyeva

Chertov

Nechaeva

et al. 2021

Forests

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In order to carry out activities aimed at conservation and rational use of forest resources; it is necessary to study the main forest-forming plant species in detail. Scots pine (Pinus sylvestris L., Pinaceae) is mainly found in the boreal forests of Eurasia and is not so often encountered in the east of the East European Plain. The aim of the study was to study the genetic diversity, structure and differentiation of Scots pine populations in the east of the East European Plain. We studied ten populations of P. sylvestris using the Inter Simple Sequence Repeats (ISSR)-based DNA polymorphism detection method. Natural populations are demonstrated by relatively high rates of genetic diversity (He = 0.167; ne = 1.279; I = 0.253). At the same time, there is a tendency for a decrease in the genetic diversity of the studied populations of P. sylvestris from west to east. Analysis of the genetic structure shows that the studied populations are highly differentiated (GST = 0.439), the intrapopulation component accounts for about 56% of the genetic diversity. Using various algorithms for determining the spatial genetic structure, it is found that the studied populations form two groups of populations in accordance with geographic location. With the help of a genetic originality coefficient, populations with specific and typical gene pools are identified. They are recommended as sources of genetic diversity and reserves for the conservation of genetic resources of the species.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Genetic Structure, Differentiation and Originality of Pinus sylvestris L. Populations in the East of the East European Plain

Vasilyeva

Chertov

Nechaeva

et al. 2021

Forests

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“…While ISSR markers (CA) 6 GT and (AGC) 6 G were reported to be polymorphic in populations of L. sibirica [ 16 ], the larches of Kuzhanovo demonstrated the same pattern as control plants. Three polymorphic bands were amplified in the larches of Kuzhanovo with primer (GTG) 5 , originally used for differentiation of L. kaempferi [ 17 ].…”

Section: Resultsmentioning

confidence: 99%

“…ISSR analysis was performed using the primers (GTG) 5 , (CA) 6 GT and (AGC) 6 G according to the protocol described by Sboeva et al [ 16 , 17 ] and visualized in 1.7% agarose gel. SSR analysis was done with primers Lar_eSSR11, Lar_eSSR54, Lar_eSSR69, Lar_eSSR78F, Lar_eSSR96, Lar_eSSR111, Lar_eSSR115, Lar_eSSR115, Lar_eSSR228, bcLK056, bcLK224, bcLK232, bcLK260 and bcLK235 ( Table 2 ), recommended by Dong et al and Kulakov et al and visualized in 10% polyacrylamide gel [ 10 , 11 ].…”

Section: Methodsmentioning

confidence: 99%

Larches of Kuzhanovo Have a Unique Mutation in the atpF–atpH Intergenic Spacer

Artyukhin

Sharifyanova

Кривошеев

et al. 2023

IJMS

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The larches of Kuzhanovo (Larix sibirica Ledeb.) are protected trees with a round crown growing in the Southern Urals. In 2020 vandals sawed the sapwood of these trees, which exposed the problem of insufficient conservation measures. Their origin and genetic characteristics have been of particular interest to breeders and scientists. The larches of Kuzhanovo were screened for polymorphisms using SSR and ISSR analyses and the sequencing of genetic markers and genes GIGANTEA and mTERF, associated with wider crown shape. A unique mutation was discovered in the atpF–atpH intergenic spacer of all protected trees, but it was absent in some of their descendants and larches with similar crown shape. Mutations were discovered in the rpoC1 and mTERF genes of all samples. Flow cytometry did not reveal any changes in genome size. Our results suggest that the unique phenotype arose from point mutations in L. sibirica, but they are yet to be found in the nuclear genome. The concurrent mutations in the rpoC1 and mTERF genes may indicate that the round crown shape originates from the Southern Urals. The atpF–atpH and rpoC1 genetic markers are not common in studies of Larix sp., but their wider use could help to establish the origin of these endangered plants. The discovery of the unique atpF–atpH mutation also allows for stronger conservation and crime detection efforts.

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Molecular Genetic Identification of Populations of Pinus sylvestris L. and Larix sibirica Ledeb. in Perm Krai Using SNP-markers

Chertov

Pystogova

Malyshkina

et al. 2020

BSP

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For the selection of identification SNP markers and identification at the population level, 5 populations of Scots pine (Pinus sylvestris L., Pinaceae) and 5 populations of the western race of Siberian larch (Larix sibirica Ledeb., Pinaceae) in the Perm Krai were studied. To determine nucleotide sequences and select identification SNP markers in 10 populations of two woody plant species of the Perm Krai, 14 pairs of nuclear and 3 pairs of chloroplast loci of P. sylvestris, as well as ten pairs of primers to 10 loci of potentially adaptively significant genes of L. sibirica were tested. Sequencing of 3 nuclear loci and 3 loci of chloroplast DNA of P. sylvestris, as well as 6 loci of nuclear adaptively significant genes of L. sibirica were sequenced. In the sequenced sequences of two plant species, 59 SNP markers were detected. Of these, 11 identification SNP markers were established, with a sufficiently high frequency of occurrence (≥0.5), suitable for identifying populations of two coniferous plant species.

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Molecular Genetic Identification of Scots Pine and Siberian Larch Populations in Perm Krai Based on Polymorphism of Issr-PCR Markers

Cited by 4 publications

References 0 publications

Genetic Structure, Differentiation and Originality of Pinus sylvestris L. Populations in the East of the East European Plain

Genetic Structure, Differentiation and Originality of Pinus sylvestris L. Populations in the East of the East European Plain

Larches of Kuzhanovo Have a Unique Mutation in the atpF–atpH Intergenic Spacer

Molecular Genetic Identification of Populations of Pinus sylvestris L. and Larix sibirica Ledeb. in Perm Krai Using SNP-markers

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