No abstract

Based on the analysis of the polymorphism of two types of microsatellite markers (ISSR and SSR), the state of gene pools of the fifteen of Siberian larch populations from three regions of the Urals: Northern, Middle and Southern was estimated. The parameters of genetic diversity were revealed, its structure was established at the intrapopulation level. To assess the uniqueness of the gene pool, we used the coefficient of genetic originality (KGO), the analysis of which revealed populations with typical and region-specific gene pools. It was established that the studied samples are generally characterized by a high level of genetic diversity. It was found that the gene pools of samples from the South Urals are characterized by the greatest specificity, the lowest values of KGO, i. e. more typical gene pools, are noted in the North Urals samples of L. sibirica, the average values of KGO are from the samples of the Middle Urals. Also, 3 unique alleles were found in the ZIL, BND, and KCH samples, in the rest, no unique markers were detected. For a comprehensive assessment of the state of the gene pools of populations, all established indicators of genetic diversity have been transferred to the scale for assessing the status of gene pools developed on the example of the studied L. sibirica populations. Based on data on genetic diversity obtained using two types of molecular markers, it was found that the gene pools of ten studied L. sibirica populations are in satisfactory condition, and five have signs of gene pool degradation. Based on the results of the study, recommendations are made on the conservation of L. sibirica genetic resources in the Urals.

The purpose of this research is the use 5 stages of standardized methodology of compression elastography based on a preliminary assessment of gray-scale images, qualitative and semi-quantitative elastography. The authors give comparative characteristics of diagnostic efficiency and reproducibility standard data and propose the elastography in 3419 people with focal lesions of the breast, thyroid, regional lymph nodes, pancreas. The article presents the ways of nationwide discussion of standardized recommendations for compression elastography. Analysis of the data shows the advantage of the proposed standardized methodology of compression elastography. The evaluation of diagnostic and prognostic values of routine and standardized methods sonoelastography was carried out. Statistics are distributed as follows: in a routine method, the specificity is 90.3%, the sensitivity is estimated at 82.4%, the accuracy - 86,7%, AUC is equal 0,892 with 95% confidence interval [0,872 – 0,974]. In a standardized method, the specificity is 93.8%, the sensitivity is 84.7%, the accuracy – 88.5%, AUC is 0,938 with 95% confidence interval [0,889 – 0,981]. The non-specificity of the standardized compression sonoelastography method allows its use in any pathology of internal organs and tissues. Strict adherence to the stages of the standardized elastography contributes to the formation of a precise protocol elastographic examination.