Molecular Hyperthermia: Spatiotemporal Protein Unfolding and Inactivation by Nanosecond Plasmonic Heating

Kang, Peiyuan; Chen, Zhuo; Nielsen, Steven O.; Hoyt, Kenneth; D’Arcy, Sheena; Gassensmith, Jeremiah J.; Qin, Zhenpeng

doi:10.1002/smll.201700841

Cited by 46 publications

(69 citation statements)

References 44 publications

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“…Carbon The very local increase in temperature may be also of interest to control the structure and folding/unfolding of proteins; indeed, Kang et al reported that in the immediate adjacent environment of AuNPs illuminated with a pulsed-laser, proteins can be denatured [104]. This interesting feature could find applications to treat diseases involving protein unfolding and aggregation.…”

Section: Lightmentioning

confidence: 99%

Nanomaterials to avoid and destroy protein aggregates

et al. 2020

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Aggregation of proteins is involved in many disorders. Besides amyloid fibrils, which mostly form in the brain, other kind of protein aggregates can lead, for example, to clots in the blood or floaters in the vitreous of the eye. This review is not only limited to amyloid diseases but aims at giving the reader a general overview on how nanomaterials can be employed to avoid and destroy protein aggregates of different nature. Thanks to their recognized versatility, nanomaterials may offer attractive features against harmful protein aggregates. In addition to their known ability to interact with proteins, we also aim at providing a state-of-the-art on how stimuli-responsive nanomaterials can be employed to destroy aggregates. Despite promising and conceptually interesting strategies on how nanomaterials can lead to the destruction of protein aggregates and the prevention of their formation, it appears clearly that many efforts still remain, however, to demonstrate in vivo feasibility and safety to pave the way for clinically relevant therapies.

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Section: Lightmentioning

confidence: 99%

Nanomaterials to avoid and destroy protein aggregates

et al. 2020

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“…It can also selectively damage deoxyribonucleic acid (DNA) in cancer cells 3 , while avoiding the damage to the healthy tissue. Recently, it was shown that the application of hyperthermia can be extended to the subcellular domain 4 .…”

Section: Microscale Direct Measurement Of Localized Photothermal Heatmentioning

confidence: 99%

Microscale direct measurement of localized photothermal heating in tissue-mimetic hydrogels

Davaji

Richie

Lee

2019

Sci Rep

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Photothermal hyperthermia is proven to be an effective diagnostic tool for cancer therapy. The efficacy of this method directly relies on understanding the localization of the photothermal effect in the targeted region. Realizing the safe and effective concentration of nano-particles and the irradiation intensity and time requires spatiotemporal temperature monitoring during and after laser irradiation. Due to uniformities of the nanoparticle distribution and the complexities of the microenvironment, a direct temperature measurement in micro-scale is crucial for achieving precise thermal dose control. In this study, a 50 nm thin film nickel resistive temperature sensor was fabricated on a 300 nm SiN membrane to directly measure the local temperature variations of a hydrogel-GNR mixture under laser exposure with 2 mK temperature resolution. The chip-scale approach developed here is an effective tool to investigate localization of photothermal heating for hyperthermia applications for in-vitro and ex-vivo models. Considering the connection between thermal properties, porosity and the matrix stiffness in hydrogels, we present our results using the interplay between matrix stiffness of the hydrogel and its thermal properties: the stiffer the hydrogel, the higher the thermal conductivity resulting in lower photothermal heating. We measured 8.1, 7.4, and 5.6 °C temperature changes (from the room temperature, 20 °C) in hydrogel models with stiffness levels corresponding to adipose (4 kPa), muscle (13 kPa) and osteoid (30 kPa) tissues respectively by exposing them to 2 W/cm 2 laser (808 nm) intensity for 150 seconds.

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“…Such models, along with efficient numerical procedures for their implementations, were developed before in the context ultrashortpulsed lasers (e.g., [13][14][15]), with an increasing range of medical applications currently in place. A number of them are connected with nanosecond pulsed laser heating techniques [16], as well as with thermoplasmonics applications in medicine [17], where the goal is to create highly specific therapies by inactivating dysfunctional protein molecules (the field known as molecular hyperthermia). Another avenue for an extension of the current model is to account for the finite speed of heat propagation through thermal relaxation models (e.g., [18][19]).…”

Section: Mathematical Modeling Of the Rfa Proceduresmentioning

confidence: 99%

Bioinformatics and Biomedical Engineering

2019

Lecture Notes in Computer Science

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The present study aims at evaluating the effects of target nerve location from the bone tissue during continuous radiofrequency ablation (RFA) for chronic pain relief. A generalized three-dimensional heterogeneous computational model comprising of muscle, bone and target nerve has been considered. The continuous RFA has been performed through the monopolar needle electrode placed parallel to the target nerve. Finite-element-based coupled thermoelectric analysis has been conducted to predict the electric field and temperature distributions as well as the lesion volume attained during continuous RFA application. The quasi-static approximation of the Maxwell's equations has been used to compute the electric field distribution and the Pennes bioheat equation has been used to model the heat transfer phenomenon during RFA of the target nerve. The electrical and thermo-physical properties considered in the present numerical study have been acquired from the well-characterized values available in the literature. The protocol of the RFA procedure has been adopted from the United States Food and Drug Administration (FDA) approved commercial devices available in the market and reported in the previous clinical studies. Temperature-dependent electrical conductivity along with the piecewise model of blood perfusion have been considered to correlate with the in-vivo scenarios. The numerical simulation results, presented in this work, reveal a strong dependence of lesion volume on the target nerve location from the considered bone. It is expected that the findings of this study would assist in providing a priori critical information to the clinical practitioners for enhancing the success rate of continuous RFA technique in addressing the chronic pain problems of bones.

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Molecular Hyperthermia: Spatiotemporal Protein Unfolding and Inactivation by Nanosecond Plasmonic Heating

Cited by 46 publications

References 44 publications

Nanomaterials to avoid and destroy protein aggregates

Nanomaterials to avoid and destroy protein aggregates

Microscale direct measurement of localized photothermal heating in tissue-mimetic hydrogels

Bioinformatics and Biomedical Engineering

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