Molecular identification of bacteria isolated from culture medium of the gold-lipped pearl oyster Pinctada maxima larvae

Abstract

Abstract. Wullur S, Napitupulu H, Wantania LL, Ginting EL, Mamangkey NGF, Smolak R, Ogello E. 2020. Molecular identification of bacteria isolated from culture medium of the gold-lipped pearl oyster Pinctada maxima larvae. Biodiversitas 21: 5291-5297. This study was conducted for the molecular identification of bacteria species isolated from culture medium of the gold-lipped pearl oyster, Pinctada maxima larvae. The pearl oysters were cultured using live-microalgae (Isochrysis sp) and fish waste diet (FWD) as food sources. Bacteria were isolated from the oyster larvae and identified based on the 16S rRNA gene sequence. The isolated bacteria were grown on agar plates and incubated at 37°C for 24 to 48 hours. Representative colonies of the bacteria were selected and cultured for molecular analysis. The 16S rRNA genes of the bacteria were amplified and the sequences were matched with the NCBI GenBank database. Seven different colonies were observed based on morphological characters. Similarity test by conducting the Basic Local Alignment Search Tool (BLAST) in the NCBI GenBank database, using the 16S rRNA gene sequences showed that the seven isolates colonies possess high similarity to five bacteria species i.e. Pseudomonas pachastrellae, Vibrio alginolyticus, Bacillus filamentosus, Bacillus cereus and Idiomarina fontislapidosi belonging to four different genera i.e. Bacillus, Staphylococcus, Vibrio, and Alteromonas.

Immune and antioxidant responses of pearl oysterPinctada maximaexposed to acute salinity stress

“…In recent years, related research on P . maxima have focused on larval breeding (Ompi et al, 2018; Wang et al, 2017; Wullur et al, 2020), growth performance (Hao et al, 2019; Wang et al, 2019), pearl cultivation (McDougall et al, 2016, 2021; Wei et al, 2017), shell materials (Alakpa et al, 2018; Anggraini et al, 2019), disease (Goncalves et al, 2017) and genetic diversity (Lind et al, 2009; Nayfa & Zenger, 2016). Previous studies on the effects of salinity on P .…”

“…The pearl oyster is mainly distributed in the central Indo-Pacific region, including the Australia, Indonesia, Japan, Micronesia, Papua New Guinea, Philippines, Polynesia and South China Sea (Liang et al, 2016). In recent years, related research on P. maxima have focused on larval breeding (Ompi et al, 2018;Wang et al, 2017;Wullur et al, 2020), growth performance (Hao et al, 2019;Wang et al, 2019), pearl cultivation (McDougall et al, 2016Wei et al, 2017), shell materials (Alakpa et al, 2018;Anggraini et al, 2019), disease (Goncalves et al, 2017) and genetic diversity (Lind et al, 2009;Nayfa & Zenger, 2016). Previous studies on the effects of salinity on P. maxima focus on growth and survival rate (Deng et al, 2013;Taylor et al, 2004), and there is still a blank about the influence of salinity stress on the immune and antioxidant indexes of P. maxima.…”

Transcriptomic Signatures of Pearl Oyster Pinctada Maxima in Response to Acute Salinity Stress

“…In comparison with salinity 30 ppt, Taylor et al (2004) pointed out that the growth of P. maxima was significantly depressed at a salinity of 25 ppt, 40 ppt, and ppt, and the results of Deng et al (2013) showed that the growth and survival rate of P. maxima larvae declined at the salinity of ppt, 24 ppt, and 21 ppt. Furthermore, in recent years, the research about genetic diversity, pearl cultivation, larval reproduction, growth performance, shell materials, and disease have been documented in P. maxima (Lind et al, 2009;Nayfa and Zenger, 2016;Ompi et al, 2018;Anggraini et al, 2019;Hao et al, 2019;Wang et al, 2019a;Wullur et al, 2020;McDougall et al, 2021). Therefore, more information is needed on the effects of salinity stress on the P. maxima, as P. maxima is often cultured in offshore areas that are subject to fluctuating salinity.…”