Molecular identification of <i>Babesia canis canis</i> genotype A in a dog from Iran

Ghasemzade, Milad; Esmaeilnejad, Bijan; Asri‐Rezaei, Siamak; Hadian, Mojtaba

doi:10.1002/vms3.630

Cited by 4 publications

(2 citation statements)

References 25 publications

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“…From Poland, a few samples were reported to carry a TT motif at this position (Lyp et al., 2015) but the sequences were not deposited in GenBank and could therefore not be included in the network analysis presented here. Recent studies from Iran showed that both genotypes are present in dogs from the country but numbers of positive dogs that were analysed was too small to speculate about their frequency (Ghasemzade et al., 2021; Khanmohammadi et al., 2021). However, Hrazdilová et al.…”

Section: Discussionmentioning

confidence: 99%

High genetic diversity of Babesia canis (Piana & Galli‐Valerio, 1895) in a recent local outbreak in Berlin/ Brandenburg, Germany

Helm

Weingart

Ramünke

et al. 2022

Transbounding Emerging Dis

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Canine babesiosis caused by Babesia canis (Piana & Galli-Valerio, 1895) is emerging in new regions in Europe since its vector Dermacentor reticulatus (Fabricius, 1794) is expanding its geographic range. In the Berlin/Brandenburg area in northeast Germany, D. reticulatus is highly abundant but in the past only one autochthonous B. canis infection was reported. Since 2015, autochthonous cases were occasionally diagnosed but numbers increased since autumn 2019. The aim of the study was to genotype autochthonous canine Babesia spp. infections from Berlin/Brandenburg.Between 04/2015 and 01/2022, 46 dogs with acute babesiosis were presented to the small animal clinic (one dog was infected twice resulting in 47 samples). There were 32 dogs that had never left Berlin/Brandenburg and 14 others that had not left the region in the 6 weeks prior to disease onset. PCRs targeting the 18S rRNA and the Bc28.1 merozoite surface antigen were positive in 47 and 42 samples, respectively. Sequencing of cloned PCR products identified all samples as B. canis with 17 18S rRNA and 12 Bc28.1 haplotypes. Based on network analysis for 18S rRNA sequences and a previously described polymorphic dinucleotide, samples were assigned to two distinct clusters. One contained 31 and the other 16 samples. Using network analysis, the Bc28.1 haplotypes could also be separated into two clusters differing by at least five polymorphisms. Analyses of sequences from multiple clones indicated the presence of up to five 18S rRNA and eight Bc28.1 haplotypes and thus high parasite variability in an individual host. The genetic diversity could suggest that the parasites in the region have multiple origins, but diversity in individual dogs and dog populations from endemic regions is unknown. The suitability of both markers for genotyping is questionable due to potential intragenomic diversity for the rRNA and high intergenomic variability for the Bc28.1 marker.

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Section: Discussionmentioning

confidence: 99%

High genetic diversity of Babesia canis (Piana & Galli‐Valerio, 1895) in a recent local outbreak in Berlin/ Brandenburg, Germany

Helm

Weingart

Ramünke

et al. 2022

Transbounding Emerging Dis

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“…Single cases of B. canis infection in dogs have been detected in countries and regions where the disease is non-endemic, including Japan, the United States, Iran, Turkey (Southeastern Turkey), Estonia, and Nigeria [ 52 , 178 , 179 , 180 , 181 ]. However, a study from North America identified 18 cases of B. canis infection among 9367 blood and tissue samples from presumably symptomatic dogs, despite this part of the world being non-endemic for D. reticulatus ticks [ 5 ].…”

Section: Prevalence Of Babesia Canismentioning

confidence: 99%

Canine Babesiosis Caused by Large Babesia Species: Global Prevalence and Risk Factors—A Review

Zygner

Gójska-Zygner²,

Bartosik

et al. 2023

Animals

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Canine babesiosis is a disease caused by protozoan pathogens belonging to the genus Babesia. Four species of large Babesia cause canine babesiosis (B. canis, B. rossi, B. vogeli, and the informally named B. coco). Although canine babesiosis has a worldwide distribution, different species occur in specific regions: B. rossi in sub-Saharan Africa, B. canis in Europe and Asia, and B. coco in the Eastern Atlantic United States, while B. vogeli occurs in Africa, southern parts of Europe and Asia, northern Australia, southern regions of North America, and in South America. B. vogeli is the most prevalent large Babesia species globally. This results from its wide range of monotropic vector species, the mild or subclinical nature of infections, and likely the longest evolutionary association with dogs. The most important risk factors for infection by large Babesia spp. include living in rural areas, kennels or animal shelters, or regions endemic for the infection, the season of the year (which is associated with increased tick activity), infestation with ticks, and lack of treatment with acaricides.

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Molecular identification of Babesia canis canis genotype A in a dog from Iran

Ghasemzade

Esmaeilnejad

Asri‐Rezaei

et al. 2021

Veterinary Medicine & Sci

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Background: Canine babesiosis is a common and clinically significant tick-borne disease caused by obligate haematozoan parasites of the genus Babesia.Purpose: To report Babesia canis canis genotype A infection in a dog. Methods:A 2-year-old female Shih Tzu dog was submitted with the history of anorexia and depression for one week and no prior surgery. Fever, anorexia, depression and vomiting as well as mucosal pallor were noticed on physical examination. Microscopic examination of the Giemsa-stained blood smear disclosed large form of Babesia, and single to four pear-shaped merozoites within erythrocytes (RBCs). The specific primers were used for detecting Babesia canis. Results:The result of PCR was confirmed by 18S rRNA gene sequence analyzing and has been registered in GenBank under following accession numbers for Babesia canis canis (MW199108). The sequences were compared to those in GenBank, and alignments showed that the B. canis canis isolate belonged to genotype A.Conclusions: This is the first description of B. canis canis genotype A in dog from Iran.

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Molecular identification of Babesia canis canis genotype A in a dog from Iran

Cited by 4 publications

References 25 publications

High genetic diversity of Babesia canis (Piana & Galli‐Valerio, 1895) in a recent local outbreak in Berlin/ Brandenburg, Germany

High genetic diversity of Babesia canis (Piana & Galli‐Valerio, 1895) in a recent local outbreak in Berlin/ Brandenburg, Germany

Canine Babesiosis Caused by Large Babesia Species: Global Prevalence and Risk Factors—A Review

Molecular identification of Babesia canis canis genotype A in a dog from Iran

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