Molecular identification of<i>Trichogramma</i>species parasitizing<i>Ostrinia nubilalis</i>in corn and pepper in south–east border of Europe

Ivezić, Aleksandar; Rugman‐Jones, Paul F.; Malausa, Thibaut; Ris, Nicolas; Ćupina, Aleksandra Ignjatović

doi:10.1080/09670874.2020.1779383

Cited by 9 publications

(16 citation statements)

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“…The use of the multiplex assay in this study again demonstrated the widespread occurrence of 2 resident species of Trichogramma parasitizing the eggs of ECB. Coupled with the findings of surveys conducted in 2016 and 2018 (Ivezić et al 2018(Ivezić et al , 2020, it is clear that Trichogramma species, particularly T. brassicae, are important, naturally occuring egg parasitoids of ECB in Kikinda and other areas of Serbia. Indeed, natural rates of parasitism by Trichogramma spp.…”

Section: Discussionmentioning

confidence: 90%

“…Previous studies of the egg parasitoids of ECB in Serbian maize resulted in the identification of just two species, T. brassicae and T. evanescens (Ivezić et al, 2020). Identification in these studies was based on DNA sequences of the ITS2 and/or COI genes.…”

Section: Multiplex Pcr Designmentioning

confidence: 99%

“…Furthermore, there are general concerns about the effects of insecticides on beneficial insects and the wider environment, and specific concerns among the general public about insecticide residues on agricultural products (Phoofolo, 1997). Due to such growing awareness, biological control methods are becoming more favored and increasingly important (Ivezić et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Serbia is one of the countries, at the southast border of Europe, that does not actively use Trichogramma species in its agricultural practices, despite evidence suggesting high activity of native populations of these insects (Ivezić et al, 2020). Not all Trichogramma species (or populations) perform equally well in terms of mass rearing or field dispersal and parasitism rates (Borba et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…An intial survey of the Kikinda region identified two species of Trichogramma (T. brassicae and T. evanescens) parasitizing ECB eggs, based on sequences of the ITS2 gene (Ivezić et al, 2018). A second, more geographically expansive study identified the same two species, again based on DNA sequences, but this time of the mitochondrial cytochrome oxidase subunit 1 (COI) gene (Ivezić et al, 2020). In both studies, T. brassicae was found to be far more abundant in maize than was T. evanescens.…”

Section: Introductionmentioning

confidence: 99%

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Rapid molecular identification of Trichogramma (Hymenoptera: Trichogrammatidae) parasitizing the eggs of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae) in Serbia

Ivezić

Rugman‐Jones

Trudić

2021

Egypt J Biol Pest Control

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Background The European corn borer (ECB), Ostrinia nubilalis (Hübner, 1796) (Lepidoptera: Crambidae), is the major pest of maize (Zea mays Linnaeus, 1753) in Serbia. One potential method for managing this pest is the augmentative release of naturally occuring egg parasitoids of the genus Trichogramma. The first step in this process is accurately identifying the naturally occuring species and estimating their natural distribution and abundance. Molecular identification, based on differences in DNA sequences, has commonly been employed for the identification of Trichogramma species. A simple, quick, and accurate molecular assay is urgently required for the identification of two common Trichogramma species, associated with ECB in Serbia: T. brassicae Bezdenko, 1968 and Trichogramma evanescens Westwood, 1833. Such an assay will facilitate an expansive survey of resident populations of Trichogramma associated with ECB across agricultural growing regions of Vojvodina province. Results A species-specific multiplex PCR assay for the 2 species was developed and validated that assay using a sample of 79 parasitoid wasps reared from ECB egg masses collected from sample sites across Vojvodina province. Trichogramma brassicae was confirmed as the dominant egg parasitoid of ECB in this region, accounting for 77 of the 79 wasps (97.47%). The remaining 2 were confirmed as T. evanescens. Trichogramma brassicae was detected at all 12 sample sites, while T. evanescens was detected at only 2 plots, Mokrin and Nakovo. Conclusions The species-specific multiplex PCR assay presented herein can provide the basis of a quick, cheap, and reliable means for identifying the species of Trichogramma that parasitize ECB egg masses in Serbia. Two currently documented species, T. brassicae and T. evanescens, are readily diagnosed by the size of the PCR product they produce in the assay. Any additional species are expected to not produce a band of a diagnostic size. Such species would subsequently be identified by sequencing, which may also allow them to be promptly incorporated into a revised assay.

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Section: Discussionmentioning

confidence: 90%

Section: Multiplex Pcr Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Rapid molecular identification of Trichogramma (Hymenoptera: Trichogrammatidae) parasitizing the eggs of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae) in Serbia

Ivezić

Rugman‐Jones

Trudić

2021

Egypt J Biol Pest Control

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Taxonomy and Biological Control: New Challenges in an Old Relationship

et al. 2023

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Biological control and taxonomy are continuously developing fields with remarkable impacts on society. At least 80 years of literature have documented this relationship, which remains essentially the same in its mutualistic nature, as well as in its major challenges. From the perspective of Brazilian taxonomists, we discuss the impacts of important scientific and social developments that directly affect research in these areas, posing new challenges for this lasting relationship. The increasing restrictions and concerns regarding the international transit of organisms require improvements in research related to risk assessment for exotic biological control agents and also stimulate prospecting within the native biota. In our view, this is a positive situation that can foster a closer relationship between taxonomists and applied entomologists, as well as local surveys and taxonomic studies that are necessary before new programs and agents can be implemented. We discuss the essential role of molecular biology in this context, as an iconic example of the synergy between applied sciences and natural history. As our society comes to need safer and more sustainable solutions for food security and the biodiversity crisis, scientific progress will build upon this integration, where biological control and taxonomy play an essential role.

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A double-spiral maze and hi-resolution tracking pipeline to study dispersal by groups of minute insects

Cointe

Burte

Perez

et al. 2023

Sci Rep

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Minute insects such as parasitic micro-wasps have high basic and applied importance for their widespread use as biocontrol agents. Their dispersal is a phenotype of particular interest. Classically, it is evaluated using field releases, but those are time consuming, costly, and their results highly variable, preventing high-throughput and repeatability. Alternatively, dispersal can be studied using small-scale assays, but those neglect important higher-scale processes. Consequently, proper evaluation of dispersal is often complicated or lacking in academic studies and biocontrol breeding programs. Here we introduce a new method, the double-spiral maze, that allows the study of spatial propagation of groups of micro-wasps at relevant scales (several hours and meters), retaining high throughput and experimental power. The method records the location of every individual at every time, enabling accurate estimates of diffusion coefficients or other dispersal metrics. We describe this affordable, scalable, and easy-to-implement method, and illustrate its application with a species of agricultural interest.

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Molecular identification ofTrichogrammaspecies parasitizingOstrinia nubilalisin corn and pepper in south–east border of Europe

Cited by 9 publications

References 45 publications

Rapid molecular identification of Trichogramma (Hymenoptera: Trichogrammatidae) parasitizing the eggs of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae) in Serbia

Rapid molecular identification of Trichogramma (Hymenoptera: Trichogrammatidae) parasitizing the eggs of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae) in Serbia

Taxonomy and Biological Control: New Challenges in an Old Relationship

A double-spiral maze and hi-resolution tracking pipeline to study dispersal by groups of minute insects

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