Molecular Identification of Tephritid Fruit Flies (Diptera: Tephritidae) Infesting Sweet Oranges in Nsukka Agro-Ecological Zone, Nigeria, Based on PCR-RFLP of COI Gene and DNA Barcoding

Onah, Ikechukwu Eugene; Eyo, Joseph Effiong; Taylor, DeMar

doi:10.4001/003.023.0225

Cited by 13 publications

(4 citation statements)

References 14 publications

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“…For example, aside from enabling the early detection of invasive species (Onah et al 2015), DNA barcoding can reveal source regions (Bellis et al 2015) and introduction patterns (Blacket et al 2015). Nagoshi et al (2011) used it to moni-tor introductions of the invasive armyworm species, Spodoptera litura and S. littoralis, into Florida, while Tyagi et al (2015) and Wei et al (2010) detected the first invasions of Thrips parvispinus and Echinothrips americanus in India and China, repectively.…”

Section: Quarantine Of Invasive Alien Speciesmentioning

confidence: 99%

“…More recently, DNA barcoding led to the recognition of a buprestid beetle, Agrilus ribesi, whose introduction to North America had been overlooked for a century (Jendek et al 2015). Barcoding has also been used to detect invasive tephritid fruit flies in Nigeria (Onah et al 2015), leafminers in Papua New Guinea (Blacket et al 2015) and Italy (Bernardo et al 2015), a stink bug (Halyomorpha halys) in Europe (Cesari et al 2015), and a cotton bug, Oxycarenus hyalinipennis, in Florida (Nagoshi et al 2012). Based on its effectiveness in species identification, the Quarantine Barcode of Life project (www.qbol.org) worked towards establishing DNA barcoding as the core technology for identifying quarantine organisms in support of plant health, and to gain its acceptance by the pest regulators as the standard method for the identification of plant pests (van de Vossenberg et al 2013).…”

Section: Quarantine Of Invasive Alien Speciesmentioning

confidence: 99%

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DNA barcodes for bio-surveillance: regulated and economically important arthropod plant pests

Ashfaq

Hebert

2016

Genome

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Many of the arthropod species that are important pests of agriculture and forestry are impossible to discriminate morphologically throughout all of their life stages. Some cannot be differentiated at any life stage. Over the past decade, DNA barcoding has gained increasing adoption as a tool to both identify known species and to reveal cryptic taxa. Although there has not been a focused effort to develop a barcode library for them, reference sequences are now available for 77% of the 409 species of arthropods documented on major pest databases. Aside from developing the reference library needed to guide specimen identifications, past barcode studies have revealed that a significant fraction of arthropod pests are a complex of allied taxa. Because of their importance as pests and disease vectors impacting global agriculture and forestry, DNA barcode results on these arthropods have significant implications for quarantine detection, regulation, and management. The current review discusses these implications in light of the presence of cryptic species in plant pests exposed by DNA barcoding.Key words: species identification, cryptic taxa, invasive species, quarantine, pest management.Résumé : Plusieurs des espèces d'arthropodes qui constituent d'importants ravageurs en agriculture en foresterie sont impossibles à distinguer sur la base morphologique au cours de certains stades de vie. Il est impossible d'en distinguer certains à tous les stades. Au cours de la dernière décennie, l'adoption du codage à barres de l'ADN s'est accrue tant pour l'identification des espèces connues que cryptiques. Bien qu'il n'y ait pas eu d'effort visant à développer une banque de références pour ces espèces, des séquences de référence sont présentement disponibles pour 77 % des 409 espèces d'arthropodes cataloguées parmi les bases de données de ravageurs importants. Outre le développement de la banque de références nécessaire à l'identification des spécimens, les études antérieures ont révélé qu'une portion significative des arthropodes nuisibles sont composés d'un ensemble de taxons apparentés. En raison de leur importance en tant que ravageurs ou vecteurs de maladies ayant un impact global en agriculture et en foresterie, les résultats du codage à barres de ces arthropodes ont des implications importantes en matière de détection, de réglementation et de gestion de quarantaines. Dans cette synthèse, les auteurs discutent des implications à la lumière de la présence d'espèces cryptiques, au sein de ravageurs de plantes, mises au jour grâce au codage à barres de l'ADN. [Traduit par la Rédaction]

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Section: Quarantine Of Invasive Alien Speciesmentioning

confidence: 99%

Section: Quarantine Of Invasive Alien Speciesmentioning

confidence: 99%

DNA barcodes for bio-surveillance: regulated and economically important arthropod plant pests

Ashfaq

Hebert

2016

Genome

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“…Citrus hosts a large number of pests worldwide, and of these, Tephritidae fruit flies may represent major pests (Onah et al, 2015). The South American fruit fly, Anastrepha fraterculus (Wiedemann, 1830) and the Mediterranean fruit fly, Ceratitis capitata (Wiedemann, 1824) (Diptera: Tephritidae) are reported as the most common fruit fly pests attacking citrus in Brazil (Silva et al, 2006;Paiva and Parra, 2013).…”

Section: Introductionmentioning

confidence: 99%

Oviposition of fruit flies (Diptera: Tephritidae) and its relation with the pericarp of citrus fruits

et al. 2017

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Fruit flies (Diptera: Tephritidae) represent a threat to fruit growing worldwide, mainly the citrus culture, however, biological studies show that fruit flies are not perfectly adapted to this host. This study investigated oviposition of Anastrepha fraterculus (Wiedemann, 1830) and Ceratitis capitata (Wiedemann, 1824) and its relation with the pericarp of citrus fruits. We evaluated the relationship between depth of oviposition of A. fraterculus and C. capitata and epicarp thickness of orange [Citrus sinensis (L.) Osbeck)] 'Navelina' and tangerine [C. reticulata (L.)] 'Clemenules' and the influence of fruit mesocarp of tangerine 'Clemenules' on oviposition of these species. The study was conducted under controlled conditions of temperature (25 ± 2 °C), relative humidity (70 ± 10% RH) and photophase (12 h). A. fraterculus and C. capitata laid their eggs in the flavedo region of orange 'Navelina' and between the albedo and flavedo of tangerine 'Clemenules'. When fruits with mesocarp exposed were offered, there was no oviposition by both fruit fly species. The results show that epicarp thickness of citrus fruits did not influence oviposition of A. fraterculus and C. capitata as oviposition did not occur only in the presence of the mesocarp, suggesting that other factors are involved in oviposition of these species.Keywords: Anastrepha fraterculus, Ceratitis capitata, citrus peel structure, epicarp, mesocarp.Oviposição de moscas-das-frutas (Diptera: Tephritidae) e sua relação com o pericarpo de frutos cítricos ResumoAs moscas-das-frutas (Diptera: Tephritidae) representam um risco à fruticultura mundial, especialmente na cultura dos citros, entretanto estudos biológicos demonstram que as moscas-das-frutas não estão perfeitamente adaptadas à estes hospedeiros. Este estudo investigou a oviposição de Anastrepha fraterculus (Wiedemann, 1830) e Ceratitis capitata (Wiedemann, 1824) e sua relação com o pericarpo de frutos cítricos. Foi avaliada a relação entre a profundidade de oviposição de A. fraterculus e de C. capitata e a espessura do epicarpo dos frutos de laranjeira [Citrus sinensis (L.) Osbeck)] 'Navelina' e tangerineira [C. reticulata (L.)] 'Clemenules' e a influência do mesocarpo de frutos de tangerineira 'Clemenules' na oviposição destas espécies. O estudo foi conduzido em condições controladas de temperatura (25 ± 2 °C), umidade relativa (70 ± 10%) e fotofase (12 horas). A. fraterculus e C. capitata depositaram ovos no flavedo de frutos de laranjeira 'Navelina' e entre o flavedo e o albedo de frutos de tangerineira 'Clemenules'. Quando oferecido frutos com mesocarpo exposto, não houve oviposição por ambas as espécies de mosca. Os resultados demonstram que a espessura do epicarpo de frutos cítricos não influenciou a oviposição de A. fraterculus e de C. capitata, a qual não ocorreu na presença apenas do mesocarpo, sugerindo que outros fatores estão envolvidos na oviposição por estas espécies.Palavras-chave: Anastrepha fraterculus, Ceratitis capitata, estrutura da casca de citros, epicarpo, mesocarpo.

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“…Containing the spread of pest fruit flies is imminently important, especially with a sharp rise in global trade. A direct result of which is the high volume of suspected tephritids associated with goods in transit or in active orchard surveys, often as larval stages [ 6 , 7 , 8 , 9 ]. Accurate species identification from larval stages is not possible, with heavy reliance on risky and time-consuming procedures such as rearing adults [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Rapid identification of Bactrocera zonata (Dip.: Tephritidae) using TaqMan real-time PCR assay

et al. 2018

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Tephritid fruit flies are ranked as one of the most damaging groups of insect pests. Morphological identification of fruit flies is mainly performed on adults due to the lack of adequate identification keys for immature stages. The peach fruit fly, Bactrocera zonata (Saunders), infests some of the principal commercial fruits and vegetables. It is, therefore important to avert its global dispersal, particularly by accurately identifying this species at ports of entry. In this study, a TaqMan real-time polymerase chain reaction (PCR) was developed for the accurate identification and sensitive detection of the peach fruit fly. A novel set of primers and probe were designed to specifically identify the mitochondrial cytochrome oxidase I (COI) gene. All specimens of peach fruit fly (including various life stages) were detected, and no cross reactivity with other tested tephritids were observed. Since this assay performed equally well with crushed insects and purified DNA, we note added efficiency by eliminating DNA extraction step. Considering the speed, specificity as well as sensitivity of the assay, Taqman real-time PCR can be used as a swift and specific method for pest species at ports of entry.

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Molecular Identification of Tephritid Fruit Flies (Diptera: Tephritidae) Infesting Sweet Oranges in Nsukka Agro-Ecological Zone, Nigeria, Based on PCR-RFLP of COI Gene and DNA Barcoding

Cited by 13 publications

References 14 publications

DNA barcodes for bio-surveillance: regulated and economically important arthropod plant pests

DNA barcodes for bio-surveillance: regulated and economically important arthropod plant pests

Oviposition of fruit flies (Diptera: Tephritidae) and its relation with the pericarp of citrus fruits

Rapid identification of Bactrocera zonata (Dip.: Tephritidae) using TaqMan real-time PCR assay

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