Molecular Identification of the Biotype of Whitefly (Bemisia tabaci) 
 Inhabiting the Eastern Region of Saudi Arabia

Alhudaib, Khalid; Rezk, Adel A.; Abdel-Bana, Babiker Mohamed Ahme; Soliman, A.M.

doi:10.3923/jbs.2014.494.500

Cited by 13 publications

(7 citation statements)

References 22 publications

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“…Moneymaker) and French beans (P. vulgaris L., cv Goal) in Plexiglas cages (40 cm × 60 cm × 80 cm) (Millenium Chuma Limited, Kenya) with fine muslin walls for more than four generations before use in experiments (Kakimoto et al, 2007;. The whiteflies were identified as T. vaporariorum through the PCR amplification of the mitochondrial 16S ribosomal RNA (rRNA) gene fragment using the WF-F (5 -CGCCTGTTTAACAAAAACAT-3 ) and WF-R (5 -CCGGTCTGAACTCAGATCACGT-3 ) primers (Frohlich et al, 1999;Alhudaib et al, 2014). The sequencing of the PCR products obtained from 10 whiteflies confirmed the identity of the species under study.…”

Section: Insect Rearingmentioning

confidence: 99%

Endophytic Colonisation of Solanum lycopersicum and Phaseolus vulgaris by Fungal Endophytes Promotes Seedlings Growth and Hampers the Reproductive Traits, Development, and Survival of the Greenhouse Whitefly, Trialeurodes vaporariorum

et al. 2021

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In the scope of mitigating the negative impacts of pesticide use and managing greenhouse whiteflies, Trialeurodes vaporariorum sustainably, 16 endophytic fungal isolates from five different genera (Beauveria, Trichoderma, Hypocrea, Bionectria, and Fusarium) were screened for their ability to colonise two preferred host plant species, namely, tomato (Solanum lycopersicum L.) and French bean (Phaseolus vulgaris L.), through seed inoculation. Seven and nine isolates were endophytic to P. vulgaris and S. lycopersicum, respectively, where significant differences in the endophytic colonisation rates were observed among the fungal isolates in P. vulgaris and its plant parts, with a significant interaction between the isolates and plant parts in S. lycopersicum. Hypocrea lixii F3ST1, Trichoderma asperellum M2RT4, Trichoderma atroviride F5S21, and T. harzianum KF2R41 successfully colonised all the plant parts of both hosts and therefore were selected and further evaluated for their endophytic persistence, effect on plant growth, and pathogenicity to T. vaporariorum adults and F1 progeny. The four endophytes remained in both host plants for the 5-week assessment with varied colonisation rates related to the strong interaction with the time, isolates, and plant parts in both hosts. The effect of the same endophytes on the different host growth parameters varied in P. vulgaris and S. lycopersicum, with T. asperellum M2RT4 not boosting the growth in both host plants while T. atroviride F5S21 resulted in enhanced shoot biomass in S. lycopersicum. T. atroviride F5S21 and T. harzianum KF2R41 inoculated S. lycopersicum plants and H. lixii F3ST1, T. asperellum M2RT4, and T. harzianum KF2R41 inoculated P. vulgaris plants had significantly lower oviposition, while nymph development in both hosts was significantly prolonged in all the endophytically–colonised plants. The endophytes H. lixii F3ST1 and T. asperellum M2RT4 significantly reduced the longevity/survival of the exposed T. vaporariorum adults and the progeny in both S. lycopersicum and P. vulgaris. The findings demonstrate the attributes of the various endophytes in host plant growth promotion as well as their effects on the life-history parameters of T. vaporariorum and could consequently be developed as potential endophytic fungal-based biopesticides for the sustainable management of the pest in S. lycopersicum and P. vulgaris cropping systems.

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Section: Insect Rearingmentioning

confidence: 99%

Endophytic Colonisation of Solanum lycopersicum and Phaseolus vulgaris by Fungal Endophytes Promotes Seedlings Growth and Hampers the Reproductive Traits, Development, and Survival of the Greenhouse Whitefly, Trialeurodes vaporariorum

et al. 2021

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“…tabaci cryptic species [ 20 , 21 ]. Nonetheless, sequence analysis of mitochondrial cytochrome oxidase I (mt-COI) gene have been most widely accepted [ 22 – 25 ]. In the current study running culture of two different homogenous population of B .…”

Section: Resultsmentioning

confidence: 99%

Copy number variation of two begomovirus acquired and inoculated by different cryptic species of whitefly, Bemisia tabaci in Okra

Barman¹,

Samanta²,

Chakraborty

et al. 2022

PLoS ONE

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The whitefly, B.tabaci is a major pest of agricultural crops which transmits begomovirus in a species-specific manner. Yellow vein mosaic disease (YVMD) and okra leaf curl disease (OLCD) caused by distinct begomovirus are a major limitation to production of okra in India. In this framework the present investigation reports, for the first time, comparative study of begomovirus species viz. yellow vein mosaic virus (YVMV) and okra enation leaf curl virus (OELCuV) ingested and egested by two cryptic species (Asia I and Asia II 5) of B.tabaci at different time interval using detached leaf assay. A gradual increase of both virus copies were observed with increased feeding exposure in Asia I and Asia II 5. Both the genetic groups of whitefly could acquire the viruses within just 5 minutes of active feeding however, a significant amount of variation was noted in virus uptake by the both. At 24 hours of active feeding Asia II 5 acquired more of YVMV whereas, Asia I ingested more OELCuV. Similarly, the genetic group acquiring higher titre of virus egested higher amount during inoculation period. On the whole, it can be presumed that Asia I is a more effective transmitter of OELCuV whereas, Asia II 5 of YVMV further suggesting increased risk of virus pandemics (both YVMV and OELCuV) in regions where Asia I and Asia II 5 is dominant.

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“…These results showed that biotype Q was found only in one region out of nine surveyed sites indicating the dominance of biotype B in all the surveyed regions. Alhudaib et al (2014) found that the B biotype is dominant in the Al-Ahsa region of Saudi Arabia where the specimens were collected from nine different locations. Al-Shehi and Khan (2013) surveyed different geographical regions in Oman during 2011-2012 for the B. tabaci biotypes using microsatellite markers Bem 23F and Bem 23 R also, identified its secondary symbionts associated with whitefly populations collected and found that only biotype B was detected from the whitefly populations which was collected from different vegetable crops and were infected with the secondary symbiont Hamiltonella sp.…”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization and Geographical Distribution of Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) in Jordan

Mdanat

Salem

Al-Anaswah

et al. 2022

JJAS

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The whitefly Bemisia tabaci (Gennadius) is a large complex of cryptic species and is considered an invasive pest transmitting many plant viruses. It is a highly polyphagous insect that causes damage and economic losses to many plant species including vegetables, cotton, ornamentals, and weeds either in the open field or the greenhouses. A two-year survey (2016 to 2018) was conducted to biotype B. tabaci for B (MEAN1) and Q (MED) biotypes putative species. 18 host plants were screened from 14 different locations with a total of 101 samples that were collected and tested. Microsatellite markers and mitochondrial cytochrome oxidase I (mt COI) gene sequencing were used. The results showed that biotype B is dominant and prevailed in all surveyed locations.

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Molecular Identification of the Biotype of Whitefly (Bemisia tabaci) Inhabiting the Eastern Region of Saudi Arabia

Cited by 13 publications

References 22 publications

Endophytic Colonisation of Solanum lycopersicum and Phaseolus vulgaris by Fungal Endophytes Promotes Seedlings Growth and Hampers the Reproductive Traits, Development, and Survival of the Greenhouse Whitefly, Trialeurodes vaporariorum

Endophytic Colonisation of Solanum lycopersicum and Phaseolus vulgaris by Fungal Endophytes Promotes Seedlings Growth and Hampers the Reproductive Traits, Development, and Survival of the Greenhouse Whitefly, Trialeurodes vaporariorum

Copy number variation of two begomovirus acquired and inoculated by different cryptic species of whitefly, Bemisia tabaci in Okra

Molecular Characterization and Geographical Distribution of Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) in Jordan

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