Molecular insights into development of <i>Trichoderma</i> interfusants for multistress tolerance enhancing antagonism against <i>Sclerotium rolfsii</i> Sacc

Hirpara, Darshna G.; Gajera, H. P.; Patel, Abha K.; Katakpara, Zinkal A.; Golakiya, B. A.

doi:10.1002/jcp.27496

Cited by 14 publications

(7 citation statements)

References 64 publications

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“…The potent Trichoderma fusant (Fu21) was derived by protoplast fusion of T.virens NBAIITvs12 (mycoparasitic) and T.koningii MTCC796 (fungicides and abiotic stress tolerant) during our earlier studies . The potent biocontrol Fu21 showing multistress tolerance capacity and enhanced antifungal activity against phytopathogen S. rolfsii was utilized for the synthesis of green Ag‐NPs.…”

Section: Methodsmentioning

confidence: 99%

“…Fungicide tolerant and potent antagonist Trichoderma fusant (Fu21) was chosen from our previous study . Green Ag‐NPs derived from the potent Fu21 were utilized for testing the in vitro bioefficacy against pathogen S. rolfsii.…”

Section: Methodsmentioning

confidence: 99%

“…Fungicide tolerant and potent antagonist Trichoderma fusant (Fu21) was chosen from our previous study. [33] Green Ag-NPs derived from the potent Fu21 were utilized for testing the in vitro bioefficacy against pathogen S. rolfsii. Mycelial pellicle of pathogen S. rolfsii grown for 6 days in PDB that contain compact hyphal growth in upper layer and loose filamentous mycelial growth at underside layer.…”

Section: Mechanism and In-vitro Bioefficacy Of Green Nanoformulatiomentioning

confidence: 99%

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Green synthesis and antifungal mechanism of silver nanoparticles derived from chitin‐ induced exometabolites of Trichoderma interfusant

Hirpara

Gajera

2019

Applied Organom Chemis

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The potent biocontrol agent Trichoderma interfusant (Fu21) derived by protoplast fusion of mycoparasitic T.virens NBAII Tvs12 and multistress (fungicides and abiotic stress) tolerant T. koningii MTCC796. The chitin‐induced exometabolites harvested by culturing the diverse and stress tolerant Trichoderma fusant (Fu21) and utilized for synthesis of green silver nanoparticles (Ag‐NPs). Green Ag‐NPs characterized for size (62.6 nm in PSA), shape (spherical with 59.66 ± 4.18 nm under SEM), stability (51.2 mv as ZETA) and purity (3.40 ke V peak corresponded to the binding energy of silver under EDAX). A Fourier transform infrared spectroscopy exhibited electromagnetic spectra of various functional groups of exometabolites conforming the synthesis of green Ag‐NPs. We investigated novel route and mechanism of mycelial degradation at minimum inhibitory concentration (MIC) of green nanoformulation (20 μg Ag.ml−1) to restrain phytopathogen Sclerotium rolfsii. The antifungal action of green Ag‐NPs on MIC at 3 DAI elevated the mycelial cell membrane leakages (sugars and proteins), lipid peroxidation, depressed the respiratory chain dehydrogenase activity and destroyed the structure of S. rolfsii mycelia (SEM morphology) which cause phytopathogen to die. The use of green Ag‐NPs as antifungal agent is considered to be eco‐friendly resource, alternate to fungicides and cost‐effective means to diminish phytopathogen S. rolfsii causing stem rot in groundnut. Further, bioefficacy of green Ag‐NPs against S. rolfsii may be tested under field condition in groundnut rhizosphere which proved sustainability and it's advancement towards greener chemistry.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Mechanism and In-vitro Bioefficacy Of Green Nanoformulatiomentioning

confidence: 99%

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Green synthesis and antifungal mechanism of silver nanoparticles derived from chitin‐ induced exometabolites of Trichoderma interfusant

Hirpara

Gajera

2019

Applied Organom Chemis

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“…However, FU28 was found weak for antagonist action and moderate for fungicide tolerance (Hirpara et al, 2019). Thus, P1, P2, FU21, and FU28 were exploited for comparative miRNA and intracellular metabolome profiling during normal growth (P1_C; P2_C; FU21_CB; FU28_CL) and biological interactions (P1_I; P2_I; FU21_IB; FU28_IL) with test pathogen S. rolfsii.…”

Section: Sources Of Phytopathogen Sclerotium Rolfsii and Biocontrol A...mentioning

confidence: 99%

Intracellular metabolomics and microRNAomics unveil new insight into the regulatory network for potential biocontrol mechanism of stress‐tolerant Tricho‐fusants interacting with phytopathogen Sclerotium rolfsii Sacc

Hirpara

Gajera

2023

Journal Cellular Physiology

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The present study employed microRNA (miRNA) sequencing and metabolome profiling of Trichoderma parental strains and fusants during normal growth and interaction with the phytopathogen Sclerotium rolfsii Sacc. In‐vitro antagonism indicated that abiotic stress‐tolerant Tricho‐fusant FU21 was examined as a potent biocontroller with mycoparasitic action after 10 days. During interaction with the test pathogen, the most abundant uprising intracellular metabolite was recognized as l‐proline, which corresponds to held‐down l‐alanine, associated with arginine and proline metabolism, biosynthesis of secondary metabolites, and nitrogen metabolism linked to predicted genes controlled by miRNAs viz., cel‐miR‐8210‐3p, hsa‐miR‐3613‐5p, and mml‐miR‐7174‐3p. The miRNAs‐ mml‐miR‐320c and mmu‐miR‐6980‐5p were found to be associated with phenylpropanoid biosynthesis, transcription factors, and signal transduction pathways, respectively, and were ascertained downregulated in potent FU21_IB compared with FU21_CB. The amino benzoate degradation and T cell receptor signaling pathways were regulated by miRNAs cel‐miR‐8210 and tca‐miR‐3824 as stress tolerance mechanisms of FU21. The intracellular metabolites l‐proline, maleic acid, d‐fructose, Myo‐inositol, arabinitol, d‐xylose, mannitol, and butane were significantly elevated as potential biocontrol and stress‐tolerant constituents associated with miRNA regulatory pathways in potent FU21_IB. A network analysis between regulatory miRNA‐predicted genes and intracellular metabolomics acknowledged possible biocontrol pathways/mechanisms in potent FU21_IB to restrain phytopathogen.

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“…The application of Trichoderma spp. against S. rolfsii has been received a great attention because of their excellent root colonization abilities, production of antifungal metabolites, and lysis of sclerotia (Hirpara et al 2019). The mechanism through which they suppress the growth of fungal pathogens includes induction of plant defense responses, production of cell wall degrading enzymes, antibiosis, mycoparasitism, and competition for nutrients and niches (Wonglom et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Effect of soil amendment with Chenopodium album dry biomass and two Trichoderma species on growth of chickpea var. Noor 2009 in Sclerotium rolfsii contaminated soil

Ali

Javaid

Shoaib

et al. 2020

Egypt J Biol Pest Control

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Sclerotium rolfsii is a soil-borne plant pathogen that causes root diseases in hundreds of plant species. It also causes collar rot disease in chickpea (Cicer arietinum L.). The present pot study was carried out to investigate the effect of soil amendment with dry biomass of a weed Chenopodium album L. and two antagonistic fungi, namely Trichoderma harzianum and T. viride, on growth and yield of chickpea variety Noor 2009 in soil infected with S. rolfsii. The pathogen-contaminated soil was amended either with 1, 2, or 3% C. album dry biomass, T. harzianum, and T. viride alone, or combinations of either of the two Trichoderma species and plant dry biomass. The lowest shoot and root dry biomass and grain yield of chickpea were recorded in S. rolfsii inoculation alone without any soil amendment (positive control). Plant growth and yield were significantly and gradually increased over positive control with an increase in C. album dry biomass application in the soil. Likewise, soil application of either of the two Trichoderma species significantly enhanced plant growth and yield over positive control under biotic stress of S. rolfsii. Combined application of either T. harzianum or T. viride with 3% dry biomass of C. album also proved highly effective in alleviating biotic stress of S. rolfsii on growth and yield of chickpea.

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Molecular insights into development of Trichoderma interfusants for multistress tolerance enhancing antagonism against Sclerotium rolfsii Sacc

Cited by 14 publications

References 64 publications

Green synthesis and antifungal mechanism of silver nanoparticles derived from chitin‐ induced exometabolites of Trichoderma interfusant

Green synthesis and antifungal mechanism of silver nanoparticles derived from chitin‐ induced exometabolites of Trichoderma interfusant

Intracellular metabolomics and microRNAomics unveil new insight into the regulatory network for potential biocontrol mechanism of stress‐tolerant Tricho‐fusants interacting with phytopathogen Sclerotium rolfsii Sacc

Effect of soil amendment with Chenopodium album dry biomass and two Trichoderma species on growth of chickpea var. Noor 2009 in Sclerotium rolfsii contaminated soil

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