Molecular mapping of a resistance-specific PCR-based marker linked to a gall midge resistance gene (Gm4t) in rice

Mohan, M.; Sathyanarayanan, P. V.; Kumar, Arvind; Srivastava, Madhulika; Nair, Suresh

doi:10.1007/s001220050625

Cited by 40 publications

(22 citation statements)

References 35 publications

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“…Markers specific for Gm8 along with markers specific for Gm2 (Nair et al 1995), Gm4(t) (Nair et al 1996) and Gm7 (Sardesai et al 2002) will be used in pyramiding these genes in different combinations in elite cultivars of rice for developing durable resistance against different biotypes of gall midge prevalent in India. Previous studies have mapped putative resistance genes in the region between Gm4(t) and Gm8 (Mohan et al 1997b;Berruyer et al 2003) and therefore the possibility exists that these could be potential candidates for the gall midge resistance gene Gm8. Interestingly, the resistant phenotype-specific fragment AR257 has strong homology to an EST clone from an Oryza sativa var.…”

Section: Discussionmentioning

confidence: 99%

“…AR257 and AS168 are the AFLP markers, and AP19 587 is the RAPD marker identified in this study. Map position of a previously mapped (see Mohan et al 1997b) gall midge resistance gene Gm4(t) is also shown. The genetic distances are based on the Rice Genome Program map (http://rgp.dna.affrc.go.…”

Section: Scar Amplification and Generation Of Phenotypespecific Polymmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Of the total nine gall midge resistance genes that are known (Gm1 to Gm9) so far, four [Gm2,Gm4(t), Gm6(t) and Gm7] have been tagged and mapped (Mohan et al 1994(Mohan et al , 1997bNair et al 1995Nair et al , 1996Katiyar et al 2001;Sardesai et al 2002). The development of these markers has been made possible using various molecular marker techniques (Mohan et al 1994(Mohan et al , 1997bNair et al 1995Nair et al , 1996. Amplified fragment length polymorphisms (AFLP) have been widely used as a DNA fingerprinting technique (Vos et al 1995) in plant genetic studies (Hill et al 1996;Mackill et al 1996;Maughen et al 1996;Hongtrakul et al 1997;Zhu et al 1998;Bonnema et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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Tagging and mapping of a rice gall midge resistance gene, Gm8, and development of SCARs for use in marker-aided selection and gene pyramiding

et al. 2004

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Using amplified fragment length polymorphisms (AFLPs) and random amplified polymorphic DNAs (RAPDs), we have tagged and mapped Gm8, a gene conferring resistance to the rice gall midge (Orseolia oryzae), a major insect pest of rice, onto rice chromosome 8. Using AFLPs, two fragments, AR257 and AS168, were identified that were linked to the resistant and susceptible phenotypes, respectively. Another resistant phenotype-specific marker, AP19(587), was also identified using RAPDs. SCAR primers based on the sequence of the fragments AR257 and AS168 failed to reveal polymorphism between the resistant and the susceptible parents. However, PCR using primers based on the regions flanking AR257 revealed polymorphism that was phenotype-specific. In contrast, PCR carried out using primers flanking the susceptible phenotype-associated fragment AS168 produced a monomorphic fragment. Restriction digestion of these monomorphic fragments revealed polymorphism between the susceptible and resistant parents. Nucleotide BLAST searches revealed that the three fragments show strong homology to rice PAC and BAC clones that formed a contig representing the short arm of chromosome 8. PCR amplification using the above-mentioned primers on a larger population, derived from a cross between two indica rice varieties, Jhitpiti (resistant parent) and TN1 (susceptible parent), showed that there is a tight linkage between the markers and the Gm8 locus. These markers, therefore, have potential for use in marker-aided selection and pyramiding of Gm8 along with other previously tagged gall midge resistance genes [ Gm2, Gm4(t), and Gm7].

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Section: Discussionmentioning

confidence: 99%

Section: Scar Amplification and Generation Of Phenotypespecific Polymmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tagging and mapping of a rice gall midge resistance gene, Gm8, and development of SCARs for use in marker-aided selection and gene pyramiding

et al. 2004

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“…Seven gall midge resistance genes have been tagged and mapped so far (Nair et al 1994;Mohan et al 1997;Katiyar et al 2001;Sardesai et al 2002). Though the potentiality of the identified markers in marker-assisted selection (MAS) has been envisioned, their effective use in real-time segregating populations other than those of the original parents used for their identification is limited.…”

Section: Introductionmentioning

confidence: 99%

Identification of flanking SSR markers for a major rice gall midge resistance gene Gm1 and their validation

et al. 2004

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Host-plant resistance is the preferred strategy for management of Asian rice gall midge (Orseolia oryzae), a serious pest in many rice-growing countries. The deployment of molecular markers linked to gall midge resistance genes in breeding programmes can accelerate the development of resistant cultivars. In the present study, we have tagged and mapped a dominant gall midge resistance gene, Gm1, from the Oryza sativa cv. W1263 on chromosome 9, using SSR markers. A progeny-tested F2 mapping population derived from the cross W1263/TN1 was used for analysis. To map the gene locus, initially a subset of the F2 mapping population consisting of 20 homozygous resistant and susceptible lines each was screened with 63 parental polymorphic SSR markers. The SSR markers RM316, RM444 and RM219, located on chromosome 9, are linked to Gm1 at genetic distances of 8.0, 4.9 and 5.9 cM, respectively, and flank the gene locus. Further, gene/marker order was also determined. The utility of the co-segregating SSR markers was tested in a backcross population derived from the cross Swarna/W1263//Swarna, and allelic profiles of these markers were analysed in a set of donor rice genotypes possessing Gm1 and in a few gall midge-susceptible, elite rice varieties.

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Molecular Mapping and Marker Assisted Selection of Traits for Crop Improvement

Varshney

Mohapatra

Sharma

Plant Biotechnology and Molecular Markers

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Molecular mapping of a resistance-specific PCR-based marker linked to a gall midge resistance gene (Gm4t) in rice

Cited by 40 publications

References 35 publications

Tagging and mapping of a rice gall midge resistance gene, Gm8, and development of SCARs for use in marker-aided selection and gene pyramiding

Tagging and mapping of a rice gall midge resistance gene, Gm8, and development of SCARs for use in marker-aided selection and gene pyramiding

Identification of flanking SSR markers for a major rice gall midge resistance gene Gm1 and their validation

Molecular Mapping and Marker Assisted Selection of Traits for Crop Improvement

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