“…DNA isolations, Southern hybridizations, PCR reactions, and sequencing: Genomic DNA preparations from fresh or lyophilized leaf tissue, Southern analyses, and RAPD reactions were carried out as described in Sharpe et al (1995) and Mayerhofer et al (1997). PCR reactions for sequencecharacterized amplified region (SCAR) markers were performed in a Gene Amp 9700 thermocycler (Perkin-Elmer, Norwalk, CT) in 25-ml reaction volumes containing 10 mm Tris-HCl (pH 8.0); 50 mm KCl; 0.01% gelatin; 2.5 mm MgCl; 0.4 mm each of dATP, dCTP, dGTP, and dTTP; 0.2 mm each of primer; 0.5 units/reaction Taq DNA polymerase (PerkinElmer); and 25 ng of template DNA.…”