Molecular mapping of the wheat powdery mildew resistance gene Pm24 and marker validation for molecular breeding

Huang, Xiaodong; Hsam, S. L. K.; Zeller, F. J.; Wenzel, G.; Mohler, Volker

doi:10.1007/s001220051497

Cited by 139 publications

(67 citation statements)

References 39 publications

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“…Since most of the analyzed germplasm is derived from CIMMYT, Turkey or KWS LOCHOW GMBH, Germany, a higher similarity between genotypes inside of these two groups was expected. Clustering of the genotypes is in accordance with research of Huang et al (2000) where they concluded that all genotypes that originating from same geographic region did not cluster in the same group. Although in our study the grouping pattern is very origin heterogeneous in each group, the grouping pattern of some genotypes appeared to be associated, to some extent.…”

Section: Discussionsupporting

confidence: 87%

Assessing genetic diversity of wheat genotypes from different origins by SNP markers

Španić¹,

Korzun²,

Ebmeyer³

2016

Cereal Research Communications

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Genetic diversity was investigated in a set of eleven hexaploid wheat genotypes originated from CIMMYT, Turkey in comparison with some modern European mostly originated from KWS wheat breeding program using 24 wheat SNP markers. The lowest and highest genetic dissimilarities were observed between genotypes Opus and LDO 330/06, KWS Salix and LDO 330/06, respectively. Based on cluster analysis, 38 wheat genotypes were grouped in two main clusters. Although the grouping pattern is very origin heterogeneous in each group, the grouping pattern of some genotypes appeared to be associated, to some extent. Principal coordinate analysis (PCoA) was used as an alternative way of visualizing the genotypic data. The first, second and third principal components explained 17.79%, 14.39% and 12.24% of the variation, respectively. This study can also be an indicator for breeders to evolve genotypes with diverse genetic background to achieve sustainability in wheat production, to get favorable heterotic combinations in a wheat improvement program.

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Section: Discussionsupporting

confidence: 87%

Assessing genetic diversity of wheat genotypes from different origins by SNP markers

Španić¹,

Korzun²,

Ebmeyer³

2016

Cereal Research Communications

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“…BSA has been used to identify markers linked to important disease resistance traits in wheat (Eastwood et al 1994;Chagué et al 1999;Huang et al 2000). However, even though BSA in the CPI133872 × Janz population successfully detected a small number of putative markers associated with nematode resistance, the polygenic nature of the resistance traits resulted in a number of single-marker associations that were false positives.…”

Section: Discussionmentioning

confidence: 99%

Identification of quantitative trait loci for resistance to two species of root-lesion nematode (Pratylenchus thornei and P. neglectus) in wheat

Zwart

Thompson²,

Godwin

2005

Aust. J. Agric. Res.

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Abstract. Pratylenchus thornei and P. neglectus are two species of root-lesion nematode that cause substantial yield losses in wheat. No commercially available wheat variety has resistance to both species. A doubled-haploid population developed from a cross between the synthetic hexaploid wheat line CPI133872 and the bread wheat Janz was used to locate and tag quantitative trait loci (QTLs) associated with resistance to both P. thornei and P. neglectus. Wheat plants were inoculated with both species of nematode in independent replicated glasshouse trials repeated over 2 years. Known locations of wheat microsatellite markers were used to construct a framework map. After an initial single-marker analysis to detect marker-trait linkages, chromosome regions associated with putative QTLs were targetted with microsatellite markers to increase map density in the chromosome regions of interest. In total, 148 wheat microsatellite markers and 21 amplified fragment length polymorphism markers were mapped. The codominant microsatellite marker Xbarc183 on the distal end of chromosome 6DS was allelic for resistance to both P. thornei and P. neglectus. The QTL were designated QRlnt.lrc-6D.1 and QRlnn.lrc-6D.1, for the 2 traits, respectively. The allele inherited from CPI133872 explained 22.0-24.2% of the phenotypic variation for P. thornei resistance, and the allele inherited from Janz accounted for 11.3-14.0% of the phenotypic variation for P. neglectus resistance. Composite interval mapping identified markers that flank a second major QTL on chromosome 6DL (QRlnt.lrc-6D.2) that explained 8.3-13.4% of the phenotypic variation for P. thornei resistance. An additional major QTL associated with P. neglectus resistance was detected on chromosome 4DS (QRlnn.lrc-4D.1) and explained a further 10.3-15.4% of the phenotypic variation. The identification and tagging of nematode resistance genes with molecular markers will allow appropriate allele combinations to be selected, which will aid the successful breeding of wheat with dual nematode resistance.Additional keywords: synthetic hexaploid wheat, doubled haploids, composite interval mapping. IntroductionTwo species of root-lesion nematode, Pratylenchus thornei Sher and Allen and P. neglectus (Rensch) Filipjev and Schuurmans Stekhoven, cause substantial yield losses in cereal crops throughout the world. High populations of P. thornei cause up to 85% yield loss in wheat (Triticum aestivum L.) varieties (Thompson et al. 1993). Similarly, P. neglectus causes up to 27% yield loss in wheat varieties (Taylor et al. 1999). In Australia, annual lost wheat production caused by P. thornei and P. neglectus is estimated at $36 million and $12 million, respectively (Brennan and Murray 1998).Glasshouse screening of wheat lines inoculated with pure cultures of nematodes has suggested that the genetic mechanisms that confer resistance to P. thornei do not confer resistance to P. neglectus (Farsi et al. 1995;Taylor et al.

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“…Molecular markers are known to be useful in the process of detection of the disease resistance genes, especially in genotypes where the genetic back ground has not been clarified as is the case for most commercial cultivars. The genes Pm24, Pm35, and Pm37 were approved to be effective genes against the disease in the regions with similar ecological condition as Egypt in the world (Huang et al, 2000;Langridge et al, 2001;Miranda et al, 2007;Perugini et al, 2008). Therefore, this study was conducted to identify those powdery mildew resistance genes i.e.…”

Section: Introductionmentioning

confidence: 99%

Identification of Pm24, Pm35 and Pm37 in thirteen Egyptian bread wheat cultivars using SSR markers

Emara

Omar

El-Shamy³

et al. 2016

Ciênc. agrotec.

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Powdery mildew of wheat (Triticum spp.) caused by Blumeria graminis f.sp. tritici (DC) E.O. Speer Em. Marchal is one of the most important bread wheat diseases in Egypt. All the Egyptian common bread wheat cultivars are susceptible to that disease at seedling and adult stages. Breeding of resistant cultivars is the most economical and environmentally safe method to eliminate the disease and reduce crop losses. Combinations of two or more effective resistance genes may lead to better, more durable resistance to that disease. Eight Pm genes i.e. Pm2, Pm6, Pm12, Pm16, Pm24, Pm35, Pm36 and Pm37 out of 21 powdery mildew monogenic wheat lines (Pm) were resistant to 42 individual isolates of powdery mildew collected from different governorates in the Nile Delta area, Egypt, at seedling and adult stages. Only four DNA specific SSR markers (Xgwm337, Xcfd7 linked to Pm24, Pm35 and Xgwm332, Xwmc790) linked to Pm37 resistance genes were selected to detect these genes in 13 Egyptian common bread wheat cultivars. This study reveals the absence of Pm24, Pm35 and Pm37 in all the 13 Egyptian bread wheat cultivars. These results gave evidence that the Egyptian cultivars are not having resistance genes and need to further incorporate one, two or more resistant genes in a single genotype as all commercial cultivars defeated by the pathogen.

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Molecular mapping of the wheat powdery mildew resistance gene Pm24 and marker validation for molecular breeding

Cited by 139 publications

References 39 publications

Assessing genetic diversity of wheat genotypes from different origins by SNP markers

Assessing genetic diversity of wheat genotypes from different origins by SNP markers

Identification of quantitative trait loci for resistance to two species of root-lesion nematode (Pratylenchus thornei and P. neglectus) in wheat

Identification of Pm24, Pm35 and Pm37 in thirteen Egyptian bread wheat cultivars using SSR markers

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