Molecular markers located on the DGAT1, CAST, and LEPR genes and their associations with milk production and fertility traits in Holstein cattle

Hill, Rebecca A.; Canal, A.M.; Bondioli, K. R.; Morell, Robert J.; Garcia, Matthew

doi:10.4238/gmr.15017794

Cited by 11 publications

(5 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One approach to identify causative mutations is the candidate gene approach. Among the genes with SNP associated with reproductive traits in cattle are DGAT1, CAST, GHR, and LEPR for services per conception and DPR (Garcia et al, 2006;Schneider et al, 2013;Hill et al, 2016); IGF1 for resumption of ovarian cyclicity (Nicolini et al, 2013); and HSPA1L, STAT1, STAT3, PARM1, and WBP1 for fertilization and embryonic development during the preimplantation period (Khatib et al, 2009;Cochran et al, 2013b).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, a few of the genes found to be associated with reproductive traits across studies evaluated here have also been reported to be associated with reproductive traits in other reports. The SNP in CAST has been associated with genetic merit for DPR, days open, and productive life (Garcia et al, 2006;Hill et al, 2016). The SNP in PCCB, PMM2, and TBC1D24 have previously been associated with percentage of cleaved embryos that develop to the blastocyst stage (Cochran et al, 2013b).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Association of single nucleotide polymorphisms in candidate genes previously related to genetic variation in fertility with phenotypic measurements of reproductive function in Holstein cows

Ortega

Denicol

Cole

et al. 2017

Journal of Dairy Science

View full text Add to dashboard Cite

Many genetic markers related to health or production traits are not evaluated in populations independent of the discovery population or related to phenotype. Here we evaluated 68 single nucleotide polymorphisms (SNP) in candidate genes previously associated with genetic merit for fertility and production traits for association with phenotypic measurements of fertility in a population of Holstein cows that was selected based on predicted transmitting ability (PTA) for daughter pregnancy rate (DPR; high, ≥1, n = 989; low, ≤ -1.0, n = 1,285). Cows with a high PTA for DPR had higher pregnancy rate at first service, fewer services per conception, and fewer days open than cows with a low PTA for DPR. Of the 68 SNP, 11 were associated with pregnancy rate at first service, 16 with services per conception, and 19 with days open. Single nucleotide polymorphisms in 12 genes (BDH2, BSP3, CAST, CD2, CD14, FUT1, FYB, GCNT3, HSD17B7, IBSP, OCLN, and PCCB) had significant associations with 2 fertility traits, and SNP in 4 genes (CSPP1, FCER1G, PMM2, and TBC1D24) had significant associations with each of the 3 traits. Results from this experiment were compared with results from 2 earlier studies in which the SNP were associated with genetic estimates of fertility. One study involved the same animals as used here, and the other study was of an independent population of bulls. A total of 13 SNP associated with 1 or more phenotypic estimates of fertility were directionally associated with genetic estimates of fertility in the same cow population. Moreover, 14 SNP associated with reproductive phenotype were directionally associated with genetic estimates of fertility in the bull population. Nine SNP (located in BCAS, BSP3, CAST, FUT1, HSD17B7, OCLN, PCCB, PMM2, and TBC1D24) had a directional association with fertility in all 3 studies. Examination of the function of the genes with SNP associated with reproduction in more than one study indicates the importance of steroid hormones and immune function as determinants of reproductive function. All but 1 of the 68 evaluated SNP were variable in 11 breeds besides Holstein, indicating the potential effects of these SNP on reproductive function across breeds of cattle.

show abstract

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

Association of single nucleotide polymorphisms in candidate genes previously related to genetic variation in fertility with phenotypic measurements of reproductive function in Holstein cows

Ortega

Denicol

Cole

et al. 2017

Journal of Dairy Science

View full text Add to dashboard Cite

show abstract

“…Some studies have reported that SNPs in DGAT1 are significantly associated with milk yield, high milk fat yield, high fat, and high protein in dairy cows and buffaloes (Lacorte et al, 2006;Tantia et al, 2006;Hill et al, 2016). However, the SNPs found in this study were in an intron region, which is not important for amino acid chains on proteins, although some polymorphisms found in the intron regions of enzyme genes can affect enzyme functionality, as reported by Buraczynska et al (2004) for the endothelial synthase nitric oxide gene and by Sachse et al (1999) for the cytochrome isoenzyme P450 gene (CYP1A2).…”

Section: Discussionmentioning

confidence: 99%

“…Another study showed that minisatellite markers in DGAT1 are associated with milk constituents in buffaloes (Cardoso et al, 2015). Tăbăran et al (2015) reported the influence of polymorphisms in DGAT1 on the percentage and profile of milk fat in Romanian Holstein cattle; therefore, they should be used in marker-assisted selection (Hill et al, 2016). Lacorte et al (2006) used polymerase chain reaction (PCR)-restriction fragment length polymorphism to reveal that SNPs in DGAT1 exhibited a high frequency of the A allele in Holstein and Gyr x Holstein F 1 cattle.…”

Section: Introductionmentioning

confidence: 99%

Polymorphisms in the DGAT1 gene in buffaloes (Bubalus bubalis) in the Amazon

et al. 2016

View full text Add to dashboard Cite

ABSTRACT. Water buffaloes (Bubalus bubalis) are quite well adapted to climatic conditions in the Amazon, and in this biome, they are noted for the considerable amount of meat and milk they produce and how hard they are able to work. Because of a lack of research dedicated to improving the rearing of buffaloes in the Amazon, the objective of this study was to genetically characterize the Murrah and Mediterranean breeds, as well as a mixed-breed population, based on polymorphisms in the diacylglycerol O-acyltransferase 1 gene (DGAT1), and associate the genotypes with milk production. By using the polymerase chain reaction-single-strand conformation polymorphism technique, the alleles A (0.79), B (0.20), and D (0.01) were found in the Murrah breed. In the Mediterranean and mixed-breed buffaloes, we found .03), and the Mediterranean and mixed-breed buffaloes had the genotypes AA (0.44) and (0.61), AB (0.50) and (0.31), and BB (0.06) and (0.08), respectively. For the Murrah, Mediterranean, and mixedbreed buffaloes, respectively, the expected heterozygosity values were 0.34, 0.43, and 0.35, the inbreeding coefficients were 0.78, -0.15, and 0.17, and the Hardy-Weinberg probabilities were 0.70, 0.67, and 0.52. The genotypes evaluated did not have an effect on milk production; however, the single nucleotide polymorphisms can be used in studies on genetic variability.

show abstract

“…La degradación proteolítica pos-mortem ocurre durante la conversión de músculo a carne en el periodo de almacenamiento a temperaturas de refrigeración y es regulada principalmente por el sistema calpaína (CAPN) -calpastatina (CAST) (Ba et al, 2015); por tanto, polimorfismos de un solo nucleótido (SNP) en estos genes han sido asociados con terneza de la carne (White et al, 2005; Allais et al, 2011; Savaºçi y Atasoy, 2016), aunque también con características reproductivas y de producción de leche (Hill et al, 2016).…”

Section: Introductionunclassified

Polimorfismos de los genes calpaína y calpastatina en el ganado criollo colombiano Hartón del Valle

Lenis

Ramos

Londoño

et al. 2018

Rev. investig. vet. Perú

View full text Add to dashboard Cite

El objetivo del presente trabajo fue caracterizar el polimorfismo de nucleótido simples 316 y 4751 del gen CAPN y 2959 y CAST1 del gen CAST en la raza bovina criolla colombiana Hartón del Valle. Se genotiparon mediante PCR-RFLP 126 individuos para los cuatro loci. Todos los marcadores fueron polimórficos. El genotipo CC del locus CAPN-316 y el heterocigoto en los otros tres loci tuvieron mayor frecuencia. Las frecuencias de los alelos asociados positivamente con calidad de la carne fueron de C= 21.8 ± 17.5% en CAPN-316, C = 48.4 ± 10.1% en CAPN-4751, T = 52.5 ± 11.4% en CAST-1 y A = 65.1 ± 15.5% en CAST-2959. En todo los loci la Ho (0.599 ± 0.248) fue más alta que la He (0.410 ± 0.081) con valores negativos de FIS y FIT y con desviaciones de EHW solo en CAPN-4751. El valor de FST fue de 0.048 (p < 0.05) y el AMOVA mostró que la variación en estos loci se debía en mayor parte a los individuos (97%). Lo anterior puede ser indicativo de que esta raza presenta predisposición genética para la producción de carne con perfiles de calidad.

show abstract

Molecular markers located on the DGAT1, CAST, and LEPR genes and their associations with milk production and fertility traits in Holstein cattle

Cited by 11 publications

References 17 publications

Association of single nucleotide polymorphisms in candidate genes previously related to genetic variation in fertility with phenotypic measurements of reproductive function in Holstein cows

Association of single nucleotide polymorphisms in candidate genes previously related to genetic variation in fertility with phenotypic measurements of reproductive function in Holstein cows

Polymorphisms in the DGAT1 gene in buffaloes (Bubalus bubalis) in the Amazon

Polimorfismos de los genes calpaína y calpastatina en el ganado criollo colombiano Hartón del Valle

Contact Info

Product

Resources

About