Molecular markers used to determine the genetic diversity and species identification of wild plants.

Nigmatullina, N.V.; Kuluev, A. R.; Kuluev, B. R.

doi:10.31301/2221-6197.bmcs.2018-39

Cited by 4 publications

(3 citation statements)

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“…In order to increase work efficiency, and reduce costs and time in the selection works with plants, a new method of selective breeding has started in recent years: "Molecular" or "Marker-Assisted" Selection (MAS). In this case, the work is carried out in a very specific directionat the level of genes forming this or that characteristic (Nigmatullina et al, 2018;Sukhareva and Kuluev, 2018). From this point of view, the genetic characterization of beet population varieties cultivated in Armenia and their wild relatives using protein markers is up-to-date and has important scientific and practical significance.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of the Beet Distributed in Armenia Using Protein Markers

Aloyan,

Badalyan,

Melikyan

2023

OnLine Journal of Biological Sciences

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The genetic resources of plants and animals are the wealth of every country. They are a valuable starting material in the breeding processes of cultivated plants and farm animals. In order to improve the existing varieties of crops and obtain new, valuable varieties, it is very important to document, preserve and enrich their gene pool, while preserving local varieties and forms of crops, it is possible to carry out breeding works. From this point of view, the genetic characterization of beet population varieties cultivated in Armenia and their wild relatives using protein markers is up-to-date and has important scientific and practical significance. It is known that the degree of genetic diversity of a population can be estimated if the protein formulas and their frequency of occurrence in the population are known. The purpose of this research was to evaluate the genetic diversity of beet spread in Armenia, formed during the evolutionary development processes, to passport the wild species and population varieties of beet by using protein markers and offering them as starting breeding material. As a result, the preferred raw material forms were proposed in the breeding works in the direction of increasing yield, sugar level, and resistance to powdery mildew and Cercospora leaf spot diseases. Further cluster analysis will make it possible to select parental pairs. Future beet breeding efforts in the target direction must make use of the genetic and genomic resources available for efficient improvement.

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Section: Introductionmentioning

confidence: 99%

Genetic Diversity of the Beet Distributed in Armenia Using Protein Markers

Aloyan,

Badalyan,

Melikyan

2023

OnLine Journal of Biological Sciences

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“…The purpose of this study was to identity genetic polymorphism of the Cyclamen L species using Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) methods (Williams et al1990). It is known that this method was used very often to identity polymorphic biodiversity among many wild and agriculture plants species (Deragon, 1992;Weising, 2005;Dugar, 2011;Catana, 2013;Nigmatullina, 2018).…”

Section: Introductionmentioning

confidence: 99%

Rapd-Analysis of Cyclamen Spp. Genome Polymorphism

Makaradze

Meparishvili

Varshanidze

et al. 2019

CBUP

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Wild plants form the basis of biological resources both for Georgia and the whole world. A strategic task of any country is to preserve the biological diversity of plants. In the territory of Ajara, a large species diversity of plants grows, among which there are rare, endemic and relict plants. In particular, Cyclamen adzharicum. Modern systematics of wild plants in Georgia is based on classical methods of botany. In this regard, it is relevant to conduct genetic studies of species diversity and genetic polymorphism of species and populations using molecular genetic markers, in particular RAPD-PCR methods. The purpose of this study was to identify genetic polymorphism in Cyclamen L species using RAPD methods. As a result of the conducted research the 65 RAPD-markers in length from 150 to 1500 BP have been revealed. The number of the amplified fragments DNA varied depending on the primer from 6 (OPA-2) to 11 (OPB-4). The results of grouping Cyclamen adzharicum and C. coum samples allowed two clusters to be identified. In the first cluster were samples of three populations Cyclamen adzharicum and showed a low stubble in the intra-species variability. Cyclamen coum was attributed to the second cluster. The used primers gave the opportunity to identify polymorphism between the tested types of cyclamen.

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“…Also, no studies have been conducted on sequencing the genome of S. tuvensis, therefore, to identify DNA polymorphism in populations of the species, PCR-based methods can be used, which do not need any prior information about the target sequences in the genome or its parts. One of the most common methods for detecting genetic polymorphism in plants that does not require knowledge of the nucleotide sequences of the genome is ISSR analysis (Inter Simple Sequence Repeats), which uses primers usually 16 −25 bp long, they consist of tandem short 2-4 nucleotide repeats and anchored at 3' or 5' end with 1 to 4 degenerate bases extended into the flanking sequences [5,6,7]. The first stage of molecular genetic analysis is the selection of primers that are effective for studying genetic diversity.…”

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ISSR primer screening for analysis of genetic diversity among Scutellaria tuvensis (Lamiaceae) populations

Muraseva

Гусева

2021

BIO Web Conf.

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Using the Diamond DNA kit, high quality nuclear DNA was isolated from dry leaves of the endemic species Scutellaria tuvensis. The selection of primers for ISSR analysis of genetic polymorphism of natural populations is described. During the experiment, 22 primers were tested, their effectiveness was assessed on a point scale. When assessing the primers, the number of reproducible amplified DNA fragments, the clarity and brightness of the obtained fragments, and only distinctive bands were taken into account. As a result, 10 ISSR markers were selected that are the most informative for assessing the population diversity of the species.

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Molecular markers used to determine the genetic diversity and species identification of wild plants.

Cited by 4 publications

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Genetic Diversity of the Beet Distributed in Armenia Using Protein Markers

Genetic Diversity of the Beet Distributed in Armenia Using Protein Markers

Rapd-Analysis of Cyclamen Spp. Genome Polymorphism

ISSR primer screening for analysis of genetic diversity among Scutellaria tuvensis (Lamiaceae) populations

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