MOLECULAR MECHANISM OF THE CAROTENOID BIOSYNTHESIS ACTIVATION IN THE PRODUCER Streptomyces globisporus 1912

Matselyukh, B.P.; Polishchuk, L. V.; Lukyanchuk, V. V.; Golembiovska, S. A.; Lavrenchuk, V. Y.

doi:10.15407/biotech7.06.069

Cited by 8 publications

(15 citation statements)

References 11 publications

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“…As previously reported, some biosynthetic gene clusters were identified in the chromosome of the S. globisporus 1912-2. The clusters of genes for syntheses of antibiotics landomycin E and A, carotenoides and gas vesicle proteins were identified in silico in the genome of the S. globisporus 1912-2 [7,12,27]. The biosynthetic lnd-genes cluster was unique and absent in the genomes of all tested Streptomycetes.…”

Section: Resultsmentioning

confidence: 99%

“…carotenoides and some others [28,29]. Carotenoge ne sis of S. globisporus 1912-2 does not require induction by illumination or other inducers [12]. The biosynthetic gvp-genes clusters were identified in genomes of S. pratensis ATCC 33331, S. xiamenensis 318 and S. violaceusniger Tu 4113 but not in the genome of S. griseus NBRC13350 [27].…”

Section: Resultsmentioning

confidence: 99%

“…With the emergence of data indicating that most Streptomycetes contain up to 30 gene clusters involved in the synthesis of secondary metabolites, it has become clear that their biosynthetic potential is underestimated [3,8,9,11,12]. Nowadays many efforts are made to estimate this potential and to get access to the natural products, the biosynthetic pathways of which are encoded in the genomes of the corresponding Streptomyces strains [13][14][15].…”

Section: Issn 1993-6842 (On-line); Issn 0233-7657 (Print)mentioning

confidence: 99%

“…The wild type strain S. globisporus 1912 and its variants synthesize some carotenoids, antitumor antibiotics landomycin E and A and a new regulator of the antibiotics biosynthesis and morphogenesis [7,12,16]. The nucleotide sequence of S. globisporus 1912-2 chromosomal DNA was identified [7].…”

Section: Issn 1993-6842 (On-line); Issn 0233-7657 (Print)mentioning

confidence: 99%

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Similarity and dissimilarity of primary structures of some Streptomyces spp. genomes and the Streptomyces globisporus 1912-2 chromosomal DNA.

Polishchuk¹

2017

Biopolym. Cell

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Aim.To determine the similarity and dissimilarity of the nucleotide sequences of the S. globisporus 1912-2 chromosome and primary structures of genomes of some Streptomycetes strains. Methods. NCBI tools (BLAST: blastn and bl2seq: megablast) and Internet NCBI databases (Genome, Nucleotide) were used for in silico analysis of the primary structure contigs of S. grlobisporus 1912-2. Results. A few strains with significant identity of their DNA primary structures to the nucleotide sequences of the chromosomal DNA of S. globisporus 1912-2 (identity 88-97 %) and a degree of query cover (55-82 %) were identified. Primary structures of genomes of the strains S. globisporus C-1027 and S. griseus NBRC13350 were chosen as most identical to the nucleotide sequence of the S. globisporus 1912-2 chromosomal DNA. No fragments with a homologous primary structure to seven S. globisporus 1912-2 contigs were found in Streptomycetes spp. from the NCBI databases. Conclusions. S. globisporus 1912-2 strain is a member of the S. griseus clade. We detected a high biosynthetic potential of the strain S. globisporus 1912-2 due to many unique nucleotide sequences. K e y w o r d s:Streptomyces, primary structure, genome, identity, in silico analysis, clade.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Issn 1993-6842 (On-line); Issn 0233-7657 (Print)mentioning

confidence: 99%

Section: Issn 1993-6842 (On-line); Issn 0233-7657 (Print)mentioning

confidence: 99%

See 2 more Smart Citations

Similarity and dissimilarity of primary structures of some Streptomyces spp. genomes and the Streptomyces globisporus 1912-2 chromosomal DNA.

Polishchuk¹

2017

Biopolym. Cell

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“…Genetic control of the biosynthesis of the new antitumor antibiotic landomycin E and carotenoids, and molecular structure of the transcriptional regulator of antibiotic production and morphogenesis in Streptomyces globisporus 1912 were studied during last 5 years at the Department of Genetics of Microorganisms of the D.K. Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine (IMV NASU) [12][13][14][15].…”

mentioning

confidence: 99%

Sequences of Landomycin E and Carotenoid Biosynthetic Gene Clusters, and Molecular Structure of Transcriptional Regulator of Streptomyces globisporus 1912

Matselyukh¹,

Polishchuk²,

Lukyanchuk³

et al. 2016

Mikrobiol. Z.

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S. globisporus 1912 in one cluster. Twenty of these lnd genes have been sequenced for the first time. The new regulatory responce gene lndRR and the sensor kinase gene lndY1 were proposed as the members of the putative two-component system. High identity (94-95 %) was determined for the lnd genes of the 1912 strain and those of the metagenomic clone AZ97. Seven carotenoid biosynthetic genes crt of the strain 1912-4Crt were sequenced and localized in one cluster consisting of two convergent operons from 4 and 3 crt genes. High homology (93 %) of the crt gene clusters of S. globisporus 1912 and S. griseus IFO 13350 was shown. Two non-punctual repeats (NPRs) of 21 bp were identified in the sequence of crtY gene coding lycopene cyclase. It was shown that the deletion of 117 bp including the sequence between NPRs of 96 bp and one NPR from 5`-side activated the crt gene cluster and increased the production of beta carotene (6.91 mg/l) and lycopene (3.24 mg/l) by the strain 1912-4Crt. Deletion of 86 bp was revealed in the regulatory gene lndRR resulting in the deficiency of landomycin E production in the strain 1912-4Crt. The DNA sequences of crt and lnd genes of S. globisporus 1912 were submitted to the NCBI database with accession numbers KM349312 and KJ645792, respectively.S. globisporus 1912 produced a low-molecular-weight compound that, like A-factor, restored the landomycin E and streptomycin biosynthesis and sporulation of the defective mutants S. globisporus 1912-B2 and S. griseus 1439, respectively. The compound was purified by thin layer chromatography and HPLC. It had an absorption maximum at λ max = = 245 nm and a molecular mass m/z 244. On the basis of NMR spectroscopy the chemical structure of the transcriptional regulator was elucidated as the new (L)-N-methylphenylalanyl-dehydrobutyrine diketopiperazine. K e y w o r d s:Soil bacteria Streptomyces produces the majority of the known antibiotics, many of the carotenoids and transcriptional regulators [18][19][20]. The research of the new antitumor antibiotics presents an important scientific problem because of the rise of drug resistant metastatic cells from the initial tumor [4]. The

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Borrelidin-producing and root-colonizing Streptomyces rochei is a potent biopesticide for two soil-borne oomycete-caused plant diseases

Zhou,

Wang,

Anjago

et al. 2024

Biological Control

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MOLECULAR MECHANISM OF THE CAROTENOID BIOSYNTHESIS ACTIVATION IN THE PRODUCER Streptomyces globisporus 1912

Cited by 8 publications

References 11 publications

Similarity and dissimilarity of primary structures of some Streptomyces spp. genomes and the Streptomyces globisporus 1912-2 chromosomal DNA.

Similarity and dissimilarity of primary structures of some Streptomyces spp. genomes and the Streptomyces globisporus 1912-2 chromosomal DNA.

Sequences of Landomycin E and Carotenoid Biosynthetic Gene Clusters, and Molecular Structure of Transcriptional Regulator of Streptomyces globisporus 1912

Borrelidin-producing and root-colonizing Streptomyces rochei is a potent biopesticide for two soil-borne oomycete-caused plant diseases

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