Molecular mechanisms of polypeptide from Chlamys farreri protecting HaCaT cells from apoptosis induced by UVA plus UVB

Abstract

Aim: To investigate the mechanism of polypeptide from Chlamys farreri (PCF) protecting HaCaT cells from apoptosis induced by UVA plus UVB in vitro. Methods: An apoptotic model of UV irradiation-induced HaCaT cells was established. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay, agarose gel electrophoresis, biochemical methods, and Western blotting were employed in the study. Results: PCF inhibited the UV irradiation-induced apoptosis of HaCaT cells. PCF strongly reduced the intracellular reactive oxygen species level, enhanced activities of superoxide dismutase and glutathione peroxidase and increased the total anti-oxidative capacity in HaCaT cells following UV irradiation. Furthermore, we found that PCF could inhibit the phosphorylation of c-Jun amino-terminal kinase and the activity of caspase-3 in a concentrationdependent manner. Conclusion: PCF protected HaCaT cells from apoptosis induced by UVA plus UVB, mainly through decreasing the intracellular ROS level and increasing the activities of anti-oxidative enzymes to block the ROS-JNKcaspase-3-apoptosis signaling pathway.

The Anti-apoptotic Effect of Polypeptide from Chlamys farreri (PCF) in UVB-Exposed HaCaT Cells Involves Inhibition of iNOS and TGF-β1

“…In this study, we confirmed that PCF could protect HaCaT cells from UVB-induced apoptosis, which is consistent with our previous findings [9]. Furthermore, we investigated iNOS and TGF-b1 as molecular mechanism of UVBinduced apoptosis cells and PCF's anti-apoptotic effect.…”

“…The 879-Da octapeptide, consists of Pro, Asn, Ser, Thr, Arg, Hyl, Cys, and Gly, is developed by Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences. Previous studies in our lab revealed that PCF could inhibit UV-induced cell apoptosis [9], with the potential mechanism involves nitric oxide (NO) producing enzymes and NO levels [10,11]. However, many still remain unknown for PCF's antiapoptotic effect in cells exposed to UVB.…”

Gefitinib-induced epidermal growth factor receptor-independent keratinocyte apoptosis is mediated by the JNK activation pathway

“…It has been demonstrated that UVB irradiation induced phosphorylation of JNK and inhibition of JNK significantly protected against UVB‐induced apoptosis 20 . In addition, the reactive oxygen species–JNK–caspase‐3 apoptosis cascade pathway is the main signalling pathway of HaCaT cell apoptosis induced by UVA and UVB 21 . The activation of the JNK pathway leads to the upregulation of the expression of cleaved caspase‐3 in HaCaT cells irradiated by UVA and UVB.…”

Purple sweet potato pigments protect murine thymocytes from60Co γ-ray-induced mitochondria-mediated apoptosis

“…By centrifugation the supernatant was removed and 100 ml dimethyl sulfoxide (DMSO) was added. 10 min later, the absorbance was read at 570 nm in a microplate reader (Tecan Group Ltd, CH-8708 Männedorf, Switzerland) (Gao X et al 2001, Gao MQ et al 2007). …”