Molecular patterns by manipulating DNA molecules

Abstract

Study of substrate-directed ordering of long double-stranded DNA molecules on bare highly oriented pyrolytic graphite surface based on atomic force microscopy relocation imaging Manipulating DNA molecules to form molecular patterns on a nanometer scale is a subject with wide prospects. By applying a modified ''molecular combing'' technique and imaging in air with atomic force microscope, we aligned DNA molecules on a mica surface which was chemically modified with a small organic molecule, ͑3-aminopropyl͒-triethanoxysilane. Two-dimensional patterns of DNA molecules were also constructed.

Nanodissection, isolation, and PCR amplification of single DNA molecules

“…10 Subsequently, the DNA molecules were extended on the APS-Mica substrate with the modified 'Molecular Combing' technique. 11,12 After waiting for a few minutes, the coverslip was removed and the APS-mica surface was rinsed with dd water and blown dry with clean compressed air or nitrogen. Nanomanipulation was performed on a Nanoscope IIIa AFM system (Veeco/DI, Santa Barbara, CA).…”

Nanomanipulation of single DNA molecules and its applications

“…5 However, it is difficult to manipulate DNA, which is a soft and elongated molecule, to obtain a regular artificial pattern from its natural randomcoil state by solely treating it with a scanned probe. Other methods that have been developed to manipulate DNA are all at macroscopic level 6,7 and cannot lead to the creation of complex patterns at the nanometre scale as described in Refs 8-11.…”

“…1(a)) were formed by macromanipulation methods such as 'modified molecular combing'. 7 Then, a set of nanomanipulations based on scanning probe microscopes was used to create the fairly artificial nanopatterns or nanostructures from those DNA matrixes. The nanomanipulation procedures include atomic force 'cutting' (Fig.…”

Grafting of Poly(tBA) and PtBA‐b‐PMMA onto the Surface of SWNTs Using Carbanions as the Initiator

“…AFM observations were conducted with a Nanoscope III microscope, a 5 mL drop of the polymer grafted SWNTs solution was spun onto a 3-aminopropyl triethoxysilane-coated mica surface. [16] To remove impurities, the sample was washed with ten drops of water while spinning and then allowed to dry in the air and imaged by tapping mode AFM.…”