Molecular profiling of microbial communities associated with seeds of Beta vulgaris subsp. Vulgaris (sugar beet)

Dent, Katherine; Stephen, J.; Finch‐Savage, William E.

doi:10.1016/j.mimet.2003.09.001

Cited by 38 publications

(25 citation statements)

References 18 publications

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“…This highlights the bias inherent to any molecular technique that uses the PCR-based 16S rRNA gene sequence analyses for microbial community description [39]. However LH-PCR has been successfully utilized to characterize the bacterial community associated with different environments, including plants [4,20,40,41]. In present work only eight peaks were attributed to the correspondent bacteria by comparison with the LH-PCR database.…”

Section: Resultsmentioning

confidence: 92%

Endophytic bacterial community of grapevine leaves influenced by sampling date and phytoplasma infection process

et al. 2014

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BackgroundEndophytic bacteria benefit host plant directly or indirectly, e.g. by biocontrol of the pathogens. Up to now, their interactions with the host and with other microorganisms are poorly understood. Consequently, a crucial step for improving the knowledge of those relationships is to determine if pathogens or plant growing season influence endophytic bacterial diversity and dynamic.ResultsFour healthy, four phytoplasma diseased and four recovered (symptomatic plants that spontaneously regain a healthy condition) grapevine plants were sampled monthly from June to October 2010 in a vineyard in north-western Italy. Metagenomic DNA was extracted from sterilized leaves and the endophytic bacterial community dynamic and diversity were analyzed by taxon specific real-time PCR, Length-Heterogeneity PCR and genus-specific PCR. These analyses revealed that both sampling date and phytoplasma infection influenced the endophytic bacterial composition. Interestingly, in June, when the plants are symptomless and the pathogen is undetectable (i) the endophytic bacterial community associated with diseased grapevines was different from those in the other sampling dates, when the phytoplasmas are detectable inside samples; (ii) the microbial community associated with recovered plants differs from that living inside healthy and diseased plants. Interestingly, LH-PCR database identified bacteria previously reported as biocontrol agents in the examined grapevines. Of these, Burkholderia, Methylobacterium and Pantoea dynamic was influenced by the phytoplasma infection process and seasonality.ConclusionResults indicated that endophytic bacterial community composition in grapevine is correlated to both phytoplasma infection and sampling date. For the first time, data underlined that, in diseased plants, the pathogen infection process can decrease the impact of seasonality on community dynamic. Moreover, based on experimental evidences, it was reasonable to hypothesize that after recovery the restructured microbial community could maintain the main structure between seasons.

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Section: Resultsmentioning

confidence: 92%

Endophytic bacterial community of grapevine leaves influenced by sampling date and phytoplasma infection process

et al. 2014

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“…Evolutionarily, endophytes also appear to form an intermediate group between saprophytic bacteria and plant pathogens. Endophytic bacteria have been isolated from a variety of plants, because they ubiquitously inhabit most plant species, including sugar beet, several potato varieties, and poplar trees (Sessitsch et al, 2002;Dent et al, 2004;Taghavi et al, 2009). Many studies have shown that endophytes are widely present in plant tissues, such as the roots, stems, leaves, and flowers (Kobayashi and Palumbo, 2000).…”

Section: Introductionmentioning

confidence: 99%

Diversity and potential application of endophytic bacteria in ginger

Chen¹,

Chen²,

Gh³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Here, 248 endophytic bacterial strains were isolated to assess the distribution and population diversity of endophytic bacteria in ginger plants. A total of 10.4 x 10 4 to 20.2 x 10 4 CFU/g fresh weight endophytic bacteria of different growth stages were isolated. Maximum bacterium numbers were obtained at the seedling stage. A total of 107 functional strains were screened, including 17 antibacterial strains and 90 indole acetic acid-producing strains. Based on 16S rDNA sequence restriction fragment length polymorphism and 16S rDNA sequences, these 107 strains were mapped and grouped into 16 genera. Bacillus and Pseudomonas were the dominant genera; however, the bacteria belonged to a tremendous range of genera, with the highest species richness being observed at the seedling stage. Sixteen strains exhibited antimicrobial activity against Pythium myriotylum Drechsler, while 7 strains exhibited antimicrobial activity against Phyllosticta zingiberi Hori. Bacillus was the dominant antibacterial strain. Pseudomonas fluorescens, B. megaterium, and Enterobacter ludwigii produced remarkably high levels of IAA. Only a few endophytic bacterial Diversity and potential application of endophytic bacteria strains were inhibited in fresh ginger juice. Most of these strains were present during seedling stage, including Roseateles depolymerans, Chryseobacterium taiwanense, E. ludwigii, Agrobacterium larrymoorei, P. fluorescens, and Bacillus amyloliquefaciens. This study indicates that the community of endophytic bacteria in ginger changes with the synthesis of antibacterial substances.

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“…Bacterial endophytes are found in a variety of plants, such as sugar beet (Dent et al, 2004), prairie plants, agronomic crops (Zinniel et al, 2002), potato varieties , wheat (Germida and Siciliano, 2001), and rice (Sun et al, 2008). The microbial community of endophytes colonizes plant tissues and is capable of establishing interactions not only among themselves but also with invaders such as pathogens, and in this way may influence plant development.…”

Section: Introductionmentioning

confidence: 99%

Diversity of endophytic bacteria in Brazilian sugarcane

Magnani

Didonet²,

Cruz³

et al. 2010

Genet. Mol. Res.

124

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ABSTRACT. Endophytic bacteria live inside plant tissues without causing disease. Studies of endophytes in sugarcane have focused on the isolation of diazotrophic bacteria. We examined the diversity of endophytic bacteria in the internal tissues of sugarcane stems and leaves, using molecular and biochemical methods. Potato-agar medium was used to cultivate the endophytes; 32 isolates were selected for analysis. DNA was extracted and the 16S rRNA gene was partially sequenced and used for molecular identification. Gram staining, catalase and oxidase tests, and the API-20E system were used to characterize the isolates. The strains were divided into five groups, based on the 16S rRNA sequences. Group I comprised 14 representatives of the Enterobacteriaceae; group II was composed of Bacilli; group III contained one representative, Curtobacterium sp; group IV contained representatives of the Pseudomonadaceae family, and group V had one isolate with an uncultured bacterium. Four isolates were able to reduce acetylene to ethylene. Most of the bacteria isolated from the sugarcane stem and leaf tissues belonged to Enterobacteriaceae and Pseudomonaceae, respectively, demonstrating niche specificity. Overall, we found the endophytic bacteria in sugarcane to be 251 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 9 (1): 250-258 (2010) Microbial diversity in sugarcane more diverse than previously reported.

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Molecular profiling of microbial communities associated with seeds of Beta vulgaris subsp. Vulgaris (sugar beet)

Cited by 38 publications

References 18 publications

Endophytic bacterial community of grapevine leaves influenced by sampling date and phytoplasma infection process

Endophytic bacterial community of grapevine leaves influenced by sampling date and phytoplasma infection process

Diversity and potential application of endophytic bacteria in ginger

Diversity of endophytic bacteria in Brazilian sugarcane

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