Molecular studies on antimicrobial resistance genes in salmonella isolated from poultry flocks in Egypt

Tawab, Ashraf A. Abd El; Hofy, Fatma I. El; Ammar, Ahmed M.; Nasef, Soad A.; Nabil, Nehal M.

doi:10.21608/bvmj.2015.32499

Cited by 4 publications

(2 citation statements)

References 16 publications

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“…The white meat isolates were predominantly resistant to rifampicin. These results agreed with Abd El-Tawab et al who reported 100% of resistance to cephalexin and rifampicin in Salmonella isolated from chicken meats in Dubai [33]. We have noted resistance to certain antibiotics, such as ciprofloxacin; amoxicillin-clavulanic acid, amoxicillin; ticarcillin-clavulanic acid and gentamycin.…”

Section: Discussionsupporting

confidence: 92%

Prevalence and genotypic characterization of Salmonella spp. from chicken meats marketed in the province of Skikda, Algeria

Djeffal

Mamache

Elgroud

et al. 2021

J Infect Dev Ctries

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Here, we aim to determine the prevalence of Salmonella contamination of poultry meat from butcheries of the province of Skikda and to investigate antibiotic resistance. Salmonella spp. isolates were screened from 70 samples, including chicken breasts (n = 40 samples) and chicken thighs (n = 30 samples) collected from 14 butcheries. All suspected Salmonella colonies from selective media were confirmed by MALDI-TOF MS and serotyped. The susceptibility profile to 16 antibiotics was studied. According to the antibiotic susceptibility results, resistance genes were investigated by standard PCR targeting various genes such as blaSHV, blaTEM, aac3, aac6-Ibcr, aad, qnrA and qnrB. Of the 14 butcheries studied, samples from eight butcheries were contaminated with Salmonella (57.14%). 19 Salmonella strains were isolated, including five serotypes with a predominance of Kentucky serotype (n = 9), Enteridis (n = 3), followed by Heidelberg (n = 3), Virchow (n = 3), and Manhattan (n= 1). All isolates were resistant to Rifampicin (100%; n = 19), and to other antibiotics such as Ciprofloxacin (47.36%), Amoxicillin-clavulanic acid (47.36%; n = 9), Amoxicillin, (47.36%; n = 9), Ticarcillin-clavulanic acid (47.36%; n = 9), and Gentamycin (47.36%; n = 9). All isolates showing multidrug resistance (47.36%; n = 9) were positive by PCR to the blaTEM-1 β-lactamase gene, from which 8 strains carried the aminoglycoside resistance aad7 gene. However, none was positive for the tested blaSHV, Aac3, Aac6-Ibcr, qnrA, qnrB, ArmA and ArmB genes. Our findings show a worrying rate of Salmonella contamination of poultry meats.

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Section: Discussionsupporting

confidence: 92%

Prevalence and genotypic characterization of Salmonella spp. from chicken meats marketed in the province of Skikda, Algeria

Djeffal

Mamache

Elgroud

et al. 2021

J Infect Dev Ctries

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“…In contrast, the phagocytic index and phagocytic percentage of the E. coli-challenged birds showed a significant decline after 35 days of age when compared to the control group. These outcomes were consistent with those of an earlier investigation (Abd El-Tawab et al, 2015), which linked these outcomes to pathophysiological effects of bacterial endotoxins and the suppression of antibacterial defense mechanisms. These outcomes could also be linked to the immune system's weariness from an E. coli infection (Lee et al, 2015).…”

Section: Blood Biochemical Parameterssupporting

confidence: 90%

The Influence of Dietary Lysozyme on the Growth Performance, Blood Constituents, Intestinal Morphology and Resistance Against Escherichia Coli in the Broiler Chicks

Hassan,

Eid,

Farouk

et al. 2023

Egyptian Journal of Nutrition and Feeds

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ne of the main Hazardous reasons the chicken sector suffers significant financial losses is Escherichia coli (E. coli). The benefits of lysozyme addition in the diet for preventing avian colibacillosis are not well understood. The purpose of the current study was to compare the effects of lysozyme dietary supplementation with antibiotic treatment in broiler chicks infected with E. coli. Eight groups of 600 one-day-old unsexed Ross chicks were created: The first group (control) was not infected and was given a balanced diet free of additives, the second and third groups were also not infected and were treated with 50 mg and 100 mg of lysozyme, respectively, the fourth group was non-infected and treated with 10 mg avilamycin / kg diet, the fifth group was infected and non-treated, the sixth and seventh group was infected and were treated with 50 mg and 100 mg of lysozyme, respectively, and the eighth group was infected and treated with 10 mg avilamycin / kg diet. The infection by E. coli was given orally to the chicks at days 5 and 16 days of age. The experimental period was 35 days for all examined groups. The E. coli-infected group showed decreased phagocytic activity, dressing percentage, relative weight of thymus and bursa, and serum total antioxidant capacity. The liver enzymes, renal function tests, MDA, relative weights of the liver, heart, and spleen, as well as changes in gut morphology and intestinal microbial counts, were all significantly elevated in the same group. The lysozyme-pretreated infected chick showed improvements in body performance metrics in addition to a noteworthy reduction in the tests for renal and hepatic enzymes. Significant improvements were observed in gut morphology, intestinal microbial counts, antioxidant enzymes, and serum immunological parameters in the treated groups. It is possible to draw the conclusion that adding lysozyme (at 50 and 100 mg/kg) to the diet of broiler chickens improves their immune system, performance, and reduces the pathological lesions caused by E. coli infections.

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Multidrug-resistant Salmonellae isolated in Japanese quails reared in Abeokuta, Nigeria

Omoshaba

Olufemi

Ojo

et al. 2017

Trop Anim Health Prod

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Salmonellosis is a major bacterial disease causing huge economic losses in the poultry industry worldwide. This study was carried out to determine the period prevalence and antimicrobial susceptibility of Salmonella enterica in Japanese quails in Abeokuta, Nigeria. Four hundred cloacal swabs of quail birds were collected from 4 locations within Abeokuta. Salmonella was isolated from the samples using conventional methods for selective isolation of Salmonella and biochemical identification. Isolates were confirmed by polymerase chain reaction assays for the amplification and detection of Salmonella-associated virulence genes (invA and stn) using specific primers. Antimicrobial susceptibility testing was done using the Kirby-Bauer disk diffusion method. In all, Salmonella was isolated from 14 (3.5%) cloacal swabs. All 14 isolates possessed invA and stn genes. The Salmonella isolates showed resistance to tetracycline (100%), doxycycline (100%), ampicillin (100%), sulphamethoxazole (92.9%), nalidixic acid (85.8%), ceftazidime (78.6%), neomycin (64.3%), streptomycin (50%) and gentamycin (28.6%) but all the isolates were susceptible to ciprofloxacin. The isolates were resistant to at least three antimicrobials indicating multidrug resistance. The results concluded that Japanese quails harbour multidrug-resistant Salmonella which could be transmitted to humans through consumption of contaminated food or by direct and indirect contact with the carrier birds. Antimicrobial resistance could be due to overdependence on antimicrobials. Ciprofloxacin could be considered in the treatment of zoonotic Salmonellosis in humans.

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Molecular studies on antimicrobial resistance genes in salmonella isolated from poultry flocks in Egypt

Cited by 4 publications

References 16 publications

Prevalence and genotypic characterization of Salmonella spp. from chicken meats marketed in the province of Skikda, Algeria

Prevalence and genotypic characterization of Salmonella spp. from chicken meats marketed in the province of Skikda, Algeria

The Influence of Dietary Lysozyme on the Growth Performance, Blood Constituents, Intestinal Morphology and Resistance Against Escherichia Coli in the Broiler Chicks

Multidrug-resistant Salmonellae isolated in Japanese quails reared in Abeokuta, Nigeria

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