Molecular Study of Broomrape (<i>Orobanche</i>Spp.) by Rapd Analyses

Atanasova, R.; Batchvarova, R.; Todorovska, Elena; Atanassov, A.

doi:10.1080/13102818.2005.10817227

Cited by 7 publications

(6 citation statements)

References 11 publications

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“…The molecular and genetic distinctions between the O. cumana populations from different countries are studied enough [4][5][6]. With the use of various types of markers derived from the DNA polymerase chain reaction, it was shown that the broomrape populations from Bulgaria, Romania, Turkey, Yugoslavia, Central and Southern Spain, and Hungary possess small intrapopulation diversity and can be differentiated according to geographical origin [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Genetic similarity and differences between the Orobanche cumana Wallr. Populations from Russia, Kazakhstan, and Romania revealed using the markers of simple sequence repeat

Guchetl

Antonova

Tchelustnikova

2014

Russ. Agricult. Sci.

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The molecular genetic diversity of the broomrape (O. cumana) populations, parasitizing on sun flower in Russia, Romania and Kazakhstan, was studied with codominant microsatellite markers. By means of cluster analysis, the populations of broomrape were divided into two clusters, regardless of their racial com position. In the first cluster, 19 samples from Russia and Kazakhstan, representing one gene pool are grouped; the second cluster consisted of five populations from Romania, united in another pool. According to Nei, the genetic distance between the clusters was 0.137. The analysis of molecular variance revealed that 22% of total variance were caused by differences between the genetic pools and 78% appeared due to differences between the samples within the each gene pool. The pairwise comparisons performed by Wright's statistics showed that the differences between these two gene pools are sufficient (F st = 21.9%) in order to make the conclusion about the existence of small genetic differentiation between the pools. The descriptive population genetic sta tistics for each pool indicate that the broomrape populations from Russia and Kazakhstan are characterized by a higher level of intrapopulation diversity than the populations from Romania.

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Section: Introductionmentioning

confidence: 99%

Genetic similarity and differences between the Orobanche cumana Wallr. Populations from Russia, Kazakhstan, and Romania revealed using the markers of simple sequence repeat

Guchetl

Antonova

Tchelustnikova

2014

Russ. Agricult. Sci.

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“…Genetic diversity based on RAPD markers has been also assessed among Orobanche spp. (Paran et al ., ; Roman et al ., ; Atanasova et al ., ), including O. cumana (Gagne et al ., ). However, no molecular analyses have been carried out to compare races or the infection ability of O. cumana populations on sunflower genotypes carrying resistance genes.…”

Section: Introductionmentioning

confidence: 99%

Pathogenic and molecular diversity in highly virulent populations of the parasitic weedOrobanche cumana(sunflower broomrape) fromEurope

et al. 2013

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Summary The parasitic weed Orobanche cumana (sunflower broomrape) constrains sunflower production in eastern and southern Europe and in the Middle East. Although genetic resistance is the most effective control method, new parasite races evolve overcoming sunflower resistance. In this work, highly virulent populations of O. cumana were analysed for pathogenicity and genetic diversity. The virulence of 11 populations from Hungary, Romania, Spain and Turkey was assessed and compared after infection of sunflower inbred lines to differentiate races of the parasite under glasshouse conditions. Molecular diversity among and within 27 parasite populations was studied by RAPD‐PCR, UPGMA and amova analyses. Highly virulent race F was identified in Hungary, Spain and Turkey. The most virulent race (G) was also found in Turkey. The molecular analysis among highly virulent populations of O. cumana identified four molecular clusters, respectively, grouping populations from Central Spain, Hungary, South Spain and Turkey. The genetic homogeneity within parasite populations was confirmed, since no molecular divergences were found within them. This work constitutes the first geographical study of O. cumana together with pathogenicity and molecular traits inherent to each geographical group, and provides useful information for possible phylogenetic analyses of O. cumana. In addition, molecular markers associated with geographical origin could be developed and used as diagnostic tools to track new broomrape introductions into areas free of virulent races where they might represent a threat to sunflower production.

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“…isoenzymes (Verkleij et al, 1986;Castejón-Múñoz et al, 1991). Later, dominant markers such as RAPDs and ISSRs were used for estimating the genetic diversity of O. cumana populations (Katzir et al, 1996;Gagne et al, 1998Gagne et al, , 2000Benharrat et al, 2002;Roman et al, 2002;Ciuca et al, 2004;Atanasova et al, 2005). In recent years, SSRs have been used more frequently due to their significant characteristics such as codominant inheritance, multiple allele numbers, and high polymorphism levels (Pineda-Martos et al, 2013, 2014a, 2014bGuchetl et al, 2014aGuchetl et al, , 2014bMartin-Sanz et al, 2016).…”

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Genetic characterization of Orobanche cumana populations from the Thrace region of Turkey using microsatellite markers

Bilgen¹,

Barut²,

Demirbaş³

2019

Turk J Bot

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Molecular Study of Broomrape (OrobancheSpp.) by Rapd Analyses

Cited by 7 publications

References 11 publications

Genetic similarity and differences between the Orobanche cumana Wallr. Populations from Russia, Kazakhstan, and Romania revealed using the markers of simple sequence repeat

Genetic similarity and differences between the Orobanche cumana Wallr. Populations from Russia, Kazakhstan, and Romania revealed using the markers of simple sequence repeat

Pathogenic and molecular diversity in highly virulent populations of the parasitic weedOrobanche cumana(sunflower broomrape) fromEurope

Genetic characterization of Orobanche cumana populations from the Thrace region of Turkey using microsatellite markers

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