Molecular survey of tick-borne pathogens in small mammals from Brazilian Amazonia

Colle, Ana Cláudia; Mendonça, Ravena Fernanda Braga de; Maia, Maerle Oliveira; Freitas, Leodil da Costa; Witter, Rute; Marcili, Arlei; Aguiar, Daniel Moura de; Muñoz-Léal, Sebastián; Labruna, Marcelo Bahia; Rossi, Rogério Vieira; Pacheco, Richard C.

doi:10.1590/s1984-29612019086

Cited by 16 publications

(19 citation statements)

References 53 publications

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“…Regarding the other PCR assays of the present study, none of the samples yielded detectable DNA to Piroplasmorida agents. This result contrasts with previous studies that reported different Piroplasmorida haplotypes among small mammals from other regions of Brazil, including studies that employed the same PCR protocol of the present study [ 3 , 20 , 24 , 39 ]. Therefore, these contrasting results could be related to uneven distribution of Piroplasmorida agents among the populations of small mammals along different geographical regions of Brazil, due to reasons yet to be determined.…”

Section: Discussioncontrasting

confidence: 99%

Novel genotypes of Hepatozoon spp. in small mammals, Brazil

et al. 2022

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Background Small mammals (rodents and marsupials) have been poorly explored for the occurrence of apicomplexan (genus Hepatozoon and genera of the order Piroplasmorida) and Anaplasmataceae agents in Brazil. Thus, this study investigated the occurrence of Hepatozoon spp., Piroplasmorida, and Anaplasmataceae agents in small mammals in seven forest fragments in Brazil. Methods During 2015–2018, small mammals were captured in six forest fragments in the State of São Paulo (Cerrado and Atlantic Forest biomes) and one fragment in the State of Mato Grosso do Sul (Pantanal biome). Mammal blood, liver, spleen, and lung samples were tested molecularly for the presence of DNA of Hepatozoon, Piroplasmorida, and Anaplasmataceae agents. Results A total of 524 mammals were captured, comprising seven species of marsupials, 14 rodents, two carnivores, and one Cingulata. Four novel haplotypes (1, 2, 3, 4) of Hepatozoon spp. were detected in small mammals from different biomes. In São Paulo state, haplotype 1 was detected in rodents from Cerrado and a transition area of Cerrado and Atlantic Forest biomes, whereas haplotype 2 was detected in rodents from the Atlantic Forest biome. On the other hand, haplotypes 3 and 4 were restricted to rodents and marsupials, respectively, from the Pantanal biome of Mato Grosso do Sul. No host species shared more than one haplotype. Despite these distinct geographical and host associations, our phylogenetic analyses indicated that the four Hepatozoon haplotypes belonged to the same clade that contained nearly all haplotypes previously reported on rodents and marsupials, in addition to several reptile-associated haplotypes from different parts of the world. No mammal samples yielded detectable DNA of Piroplasmorida agents. On the other hand, the Anaplasmataceae-targeted polymerase chain reaction (PCR) assay amplified a sequence 100% identical to the Wolbachia pipientis endosymbiont of the rodent filarid Litomosoides galizai. Conclusions We report a variety of Hepatozoon haplotypes associated with small mammals in three Brazilian biomes: Cerrado, Atlantic Forest, and Pantanal. Through phylogenetic analyses, the Hepatozoon agents grouped in the rodent-marsupial-reptile large clade of Hepatozoon spp. from the world. The detection of a W. pipientis associated with the rodent filarid L. galizai indicates that the rodent was infected by filarial nematodes. Graphical Abstract

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Section: Discussioncontrasting

confidence: 99%

Novel genotypes of Hepatozoon spp. in small mammals, Brazil

et al. 2022

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“…Additionally, a novel piroplasmid closely related to a Babesia sp. detected in Monodelphis domestica opossums from the Brazilian Pantanal has been found in D. marsupialis opossums from the Brazilian Amazon (Soares et al, 2017), and another Piroplasmida genotype was reported in two/31 (6.45%) D. marsupialis trapped in Sinop municipality, State of Mato Grosso (Colle et al, 2019). In the present study, all white-eared opossums tested negative for Theileria/Babesia spp.…”

supporting

confidence: 65%

‘Candidatus Mycoplasma haemoalbiventris’ and tick-borne pathogens screening in white-eared opossums (Didelphis albiventris) from Curitiba and Foz do Iguaçu Cities, Paraná State, southern Brazil

Oliveira

Collere

Ferrari

et al. 2021

Rev. Bras. Parasitol. Vet.

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Hemoplasmas are epierythrocytic bacteria that infect mammals. ‘Candidatus Mycoplasma haemoalbiventris’ was detected in white-eared opossums (Didelphis albiventris) from southern and central-western Brazil. The present study aimed at: i) screening opossums for tick-borne (TBP) pathogens (Piroplasmida and Anaplasmataceae) and ii) detecting and characterizing hemoplasma species infecting opossums from Curitiba and Foz do Iguaçu cities in the Paraná State, southern Brazil. Thirty blood samples from white-eared opossums were evaluated by PCR assays. Animals were not infested by ectoparasites. The mammalian endogenous gapdh gene was consistently amplified in all samples. All opossums tested negative for Theileria/Babesia spp. and Ehrlichia/Anaplasma spp. by PCR based on 18S rRNA and 16S rRNA genes, respectively. A genus-specific PCR assay based on the 16S rRNA gene of hemoplasmas showed that three/13 (23.08%; CI 95%: 8.18-50.26%) opossums from Foz do Iguaçu were positive for hemotropic Mycoplasma sp. All opossums from Curitiba tested negative for hemoplasmas. Sequencing of both the 16S and 23S rRNA genes revealed that the animals were infected by ‘Ca. M. haemoalbiventris’. Although ‘Ca. M. haemoalbiventris’ is prevalent in opossums in Brazil, clinical signs associated with its infection and its putative vectors remain unknown.

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“…Estudos em busca de patógenos transmitidos por carrapatos já foram realizados em animais de vida livre, bem como em seus ectoparasitas no Brasil (MERINO et al, 2008;WIDMER et al, 2011;ALMEIDA et al, 2013;WOLF et al, 2016;SOARES et al, 2017a,b;MONGRUEL et al, 2017, COLLE et al, 2019, PERLES et al, 2019, MUNÕZ-LEAL et al, 2019DE OLIVEIRA et al, 2020;MUÑOZ-LEAL et al, 2021a), porém o foco em pequenos mamíferos tem sido muito menor.…”

Section: Doenças Transmitidas Por Vetores Provaram Ser Inquestionávei...unclassified

“…Por outro lado, existem muitos estudos relatando a ocorrência de espécies de Hepatozoon em carnívoros, marsupiais, roedores, répteis e anfíbios, (ANDRE et al, 2010;PERLES et al, 2019;SANTOS et al, 2020;MAIA et al, 2014;DEMONER et al, 2016). Estudos recentes em roedores e marsupiais encontraram haplótipos que nas suas análises filogenéticas aparecem em um grande clado composto por haplótipos associados a roedores, marsupiais e répteis, inferindo assim que essas espécies provavelmente estejam sim relacionadas como hospedeiros paratênicos, e se infectem através da predação (WOLF et al, 2016;COLLE et al, 2019;DEMONER et al, 2019;SILVA et al, 2017;DEMONER et al, 2016).…”

Section: Doenças Transmitidas Por Vetores Provaram Ser Inquestionávei...unclassified

“…(Rodentia: Cricetidae) e amostras de sangue de sete Didelphis albiventris (de Ribeirão No Brasil, detecções moleculares de Hepatozoon spp. em pequenos roedores e marsupiais são restritas a seis estudos (WOLF et al, 2016;COLLE et al, 2019;DEMONER et al, 2019;SILVA et al, 2017, SOUSA et al, 2017bDEMONER et al, 2016), sendo que a grande maioria desses registros foi na verdade em pequenos roedores (pelo menos 12 espécies de roedores), em contraste com apenas três registros em marsupiais: Hepatozoon sp. PCR165 em um Thylamys macrurus, H. canis em dois Didelphis albiventris e Hepatozoon sp.…”

Section: Pesquisa De Agentes Da Família Anaplasmataceaeunclassified

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>b<Pesquisa de hemoparasitas transmitidos por carrapatos em pequenos mamíferos nos estados de São Paulo e Mato Grosso do Sul>/b<

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Este trabalho é dedicado a Danilo Gonçalves Saraiva (in memorian), jovem pesquisador e idealizador inicial desse projeto, que teve sua partida precoce, mas de alguma maneira esteve presente durante cada etapa dessa jornada. AGRADECIMENTOS Esse trabalho só pode ser realizado pelo esforço de uma equipe muito bem estruturada, então o agradecimento vai a todos os participantes do projeto temático, desde pesquisadores, alunos e demais funcionários. Agradeço com muita honra a todos os animais que de alguma maneira se doaram na participação desse projeto. Agradeço a todos os funcionários do VPS, por serem sempre tão solícitos em nos apoiar nos mais diversos desafios. Em especial ao Renato e Márcio, por todo apoio laboratorial, Pedrinho por sua gentileza e carinho com os animais do biotério, e ao Danival por ser sempre tão generoso e não medir esforços em nos ajudar nas burocracias. Agradeço a todos os colegas e amigos, essa jornada durou bons anos e contar com o apoio de todos foi imprescindível. Agradeço especialmente ao apoio de Amália, Felipe K, Herbert e Piauí no inicio do processo. A Carolina que acompanhou o início e o fim, pela parceria, pela ajuda desde o primero até o último dia, risadas, campos, perrengues e desabafos. Enfim, por ser a referência que ela é, rs.

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Molecular survey of tick-borne pathogens in small mammals from Brazilian Amazonia

Cited by 16 publications

References 53 publications

Novel genotypes of Hepatozoon spp. in small mammals, Brazil

Novel genotypes of Hepatozoon spp. in small mammals, Brazil

‘Candidatus Mycoplasma haemoalbiventris’ and tick-borne pathogens screening in white-eared opossums (Didelphis albiventris) from Curitiba and Foz do Iguaçu Cities, Paraná State, southern Brazil

>b<Pesquisa de hemoparasitas transmitidos por carrapatos em pequenos mamíferos nos estados de São Paulo e Mato Grosso do Sul>/b<

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