Molecular tagging of biocontrol fungus
            <i>Trichoderma asperellum</i>
            and its colonization in soil

Xian, Hua; Liu, Lu; Li, Yahua; Yang, Yanan; Yang, Song

doi:10.1111/jam.14457

Cited by 8 publications

(4 citation statements)

References 39 publications

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“…A study suggested that Trichoderma and other beneficial microorganisms would help soil microbiome recover after soil fumigation disinfestation [ 24 ]. Also, it has been reported that Trichoderma could be used as the biocontrol fungi for plant disease prevention [ 25 ]. Not only that, it has also been documented that some Trichoderma strains could suppress pathogens like F .…”

Section: Discussionmentioning

confidence: 99%

Impact of ozonated water disinfestation on soil fungal community composition in continuous ginger field

Zhang

et al. 2022

PLoS ONE

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This study aimed to explore the impact of ozonated water (OW) disinfestation on soil fungal community composition in continuous ginger field. All soil samples were collected in continuous ginger field. There were two groups and 5 time points (0, 1, 3, 5, 9 day) in our study, including OW disinfestation treatment group (O3 group) and control group (CK group). Via internal transcribed spacer (ITS) sequencing and further analysis, the changes of fungal community composition were determined. As a result, at 0 and 9 days after aeration, the operational taxonomic units (OTUs) in O3 group were significantly higher than that in CK group. Compared with the CK group, in O3 group: the ACE and Chao1 index significantly increased on day 1, and the Shannon index significantly decreased while Simpson index significantly increased on day 0 after aeration. In O3 group, there were dynamic changes of top 10 abundance fungi from the genus-level and the growth of Trichoderma and Rhodotorula had been promoted while Hannaella was inhibited. In conclusion, OW disinfestation had complicated impacts on fungal communities in continuous ginger fields. The growth of Trichoderma and Rhodotorula has been promoted during disinfestation, which provided more reference information for soil OW disinfestation research.

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Section: Discussionmentioning

confidence: 99%

Impact of ozonated water disinfestation on soil fungal community composition in continuous ginger field

Zhang

et al. 2022

PLoS ONE

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“…The ability of antagonistic microorganisms to colonize the soil directly affects the efficacy and stability of their biocontrol effects and is also an important indicator for assessing the potential of biocontrol sources [51]. In this study, Acr.…”

Section: Discussionmentioning

confidence: 99%

Effect of Rhizospheric Fungus on Biological Control of Root Rot (Fusarium equiseti) Disease of Saposhnikovia divaricata

Han

Cui²,

Wang³

et al. 2022

Agronomy

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Saposhnikovia divaricata is a high-demand medicinal plant containing various bioactive metabolites (e.g., chromone). However, root rot disease leads to a dramatic reduction in the yield and quality of S. divaricata. The use of rhizospheric microorganisms is one of the best strategies for biological control. In this study, a total of 104 fungi isolated from the rhizospheric soil of S. divaricata plants were examined for their different antifungal properties. Subsequently, strain MR-57 was selected as a potential stock for biocontrol due to its broad-spectrum antagonistic activity against pathogens, including F. equiseti. Based on the analysis of morphological properties and rDNA internal transcribed spacers (ITSs), strain MR-57 was identified as Acrophialophora jodhpurensis (GenBank No. OK287150.1), a newly recorded species for China. In an in vitro antifungal assay, the culture filtrate of strain MR-57 significantly reduced the conidial germination rate and induced alterations in the mycelia morphology of F. equiseti, such as deformation and degradation. To assess the antifungal efficacy of MR-57 against root rot disease and the properties promoting the growth of S. divaricata, pot experiments were performed under natural outdoor conditions. The results indicated that co-inoculation with MR-57 delayed the occurrence of S. divaricata root rot and showed a control efficacy of 65.41% (p < 0.05) based on the measurement of suppressed disease lesions. Additionally, MR-57 successfully colonized and formed a stable population in the soil in which S. divaricata was grown, and it exhibited a consistently positive effect on the promotion of the growth of S. divaricata plants. In short, Acr. jodhpurensis MR-57 could be considered for the development of a potential biocontrol agent for the management of S. divaricata root rot caused by F. equiseti.

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“…Each isolate sequence was deposited in GenBank. (Xian et al, 2019). Two isolates were placed 2 cm from the edge of the Petri dish.…”

Section: Dna Extraction Amplification and Sequencingmentioning

confidence: 99%

In Vitro Evaluation of Trichoderma asperellum Isolate UGM-LHAF against Rhizoctonia solani Causing Sheath Blight Disease of Rice

Nurhayati

Suryanti

Wibowo

2021

JPTI

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Trichoderma spp. is a fungus widely used to control soil-borne pathogens, such as Rhizoctonia solani which is plant pathogenic fungi in widely host range, especially on rice. This research aimed to evaluate the ability of Trichoderma asperellum isolate UGM-LHAF against R. solani causing sheath blight disease of rice in vitro condition. Trichoderma sp. used in this research was obtained from The Biological Laboratory of Pakem, Yogyakarta, Indonesia, and Rhizoctonia sp. was obtained through isolation of diseased rice obtained from rice fields in Yogyakarta. The two isolates were characterized base on morphology and molecular identification based on ITS rDNA. The pathogenicity test of Rhizoctonia sp. was evaluated by adding four sclerotia of Rhizoctonia sp. near rice roots at 6 days after sowing. The in vitro test used dual culture and antifungal activity (0%, 10%, 25%, 50% culture filtrate of Trichoderma sp.) with three replicates of each treatment. Two isolates were identified as T. asperellum and R. solani. Sheath blight symptoms appeared after 12 days inoculation. In the in vitro test, T. asperellum isolate UGM-LHAF was able to inhibit the mycelial growth of R. solani (64.23% on dual culture and 68.5% on antifungal activity). This study suggests that T. asperellum isolate UGM-LHAF able to inhibit the growth of R. solani and can be a further potential candidate as a biocontrol agent against R. solani causing sheath blight disease of rice.

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Molecular tagging of biocontrol fungus Trichoderma asperellum and its colonization in soil

Cited by 8 publications

References 39 publications

Impact of ozonated water disinfestation on soil fungal community composition in continuous ginger field

Impact of ozonated water disinfestation on soil fungal community composition in continuous ginger field

Effect of Rhizospheric Fungus on Biological Control of Root Rot (Fusarium equiseti) Disease of Saposhnikovia divaricata

In Vitro Evaluation of Trichoderma asperellum Isolate UGM-LHAF against Rhizoctonia solani Causing Sheath Blight Disease of Rice

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