Molecular taxonomy of scopulariopsis-like fungi with description of new clinical and environmental species

Jagielski, Tomasz; Sandoval-Denis, Marcelo; Yu, Jin; Yao, Limin; Bakuła, Zofia; Kalita, Joanna; Skóra, Magdalena; Krzyściak, Paweł; Hoog, G.S. de; Guarro, Josep; Gené, Josepa

doi:10.1016/j.funbio.2016.01.014

Cited by 27 publications

(20 citation statements)

References 24 publications

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“…Previous studies have reviewed the molecular phylogenetic relationships among members of this family (Lackner and Hoog, 2011, Lackner et al., 2014, Sandoval-Denis et al., 2016), providing a broad phylogenetic backbone through a molecular overview of this group of fungi. It was demonstrated that this family shows an intricate phylogenetic structure composed of several genera that share similar ecological and morphological features (Lackner and Hoog, 2011, Lackner et al., 2014, Jagielski et al., 2016, Sandoval-Denis et al., 2016). Due to the lack of DNA sequences in public databases and the paucity of phylogenetic studies, several genera of the family, particularly those with synnematous conidiophores or conidia with germ slits were still of uncertain affinities.…”

Section: Discussionmentioning

confidence: 99%

“…However, our current and recent phylogenetic results (Sandoval-Denis et al., 2016, Jagielski et al., 2016) showed the above-mentioned genera to comprise distinct lineages. Their genetic diversity correlates with subtle morphological differences, such as the presence or absence of hyaline or pigmented annellidic conidiogenous cells, unicellular or septate annelloconidia and/or solitary conidia with or without germ slits (Table 2).…”

Section: Discussionmentioning

confidence: 99%

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Phylogeny and taxonomic revision of Microascaceae with emphasis on synnematous fungi

Sandoval-Denis

Guarro

Cano-Lira

et al. 2016

Studies in Mycology

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The taxonomy of the synnematous genera Cephalotrichum, Doratomyces and Trichurus, and other related genera Gamsia, Wardomyces and Wardomycopsis, has been controversial and relies mainly on morphological criteria. These are microascaceous saprobic fungi mostly found in air and soil and with a worldwide distribution. In order to clarify their taxonomy and to delineate generic boundaries within the Microascaceae, we studied 57 isolates that include clinical, environmental and all the available ex-type strains of a large set of species by means of morphological, physiological and molecular phylogenetic analyses using DNA sequence data of four loci (the ITS region, and fragments of rDNA LSU, translation elongation factor 1α and β-tubulin). The results demonstrate that Cephalotrichum, Doratomyces and Trichurus are congeneric and the genus Cephalotrichum is accepted here with Echinobotryum as a further synonym. The genera Acaulium and Fairmania, typified by A. albonigrescens and F. singularis, respectively, are distinct from Microascus and Scopulariopsis, Gamsia is distinct from Wardomyces, and Wardomycopsis is confirmed as a separate genus in the Microascaceae. Two new species of Cephalotrichum are described as C. brevistipitatum and C. hinnuleum. Nine new combinations are proposed, i.e. Acaulium acremonium, A. caviariforme, Cephalotrichum asperulum, C. columnare, C. cylindricum, C. dendrocephalum, C. gorgonifer, Gamsia columbina and Wardomyces giganteus. A neotype is designed for C. stemonitis. Lectotypes and epitypes are designated for A. acremonium, A. albonigrescens, C. gorgonifer, C. nanum and W. anomalus. Cephalotrichum cylindricum, C. microsporum, F. singularis and Gamsia columbina are also epitypified with new specimens. Descriptions of the phenotypic features and dichotomous keys for identification are provided for accepted species in the different genera.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Phylogeny and taxonomic revision of Microascaceae with emphasis on synnematous fungi

Sandoval-Denis

Guarro

Cano-Lira

et al. 2016

Studies in Mycology

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“…The tub2 sequences were omitted in the genus phylogeny because of alignment difficulties. Based on former phylogenetic studies (Jagielski et al., 2016, Sandoval-Denis et al., 2016) Pithoascus stoveri (CBS 176.71) was used as out-group in the Microascus phylogeny, Pseudoscopulariopsis schumacheri (CBS 435.86) in the Scopulariopsis phylogeny, and Cephalotrichum stemonitis (CBS 103.19) in the genus phylogeny.…”

Section: Methodsmentioning

confidence: 99%

“…Subsequently, these habitats are plotted behind the species name, to which the isolate belongs, in the genus phylogeny tree. The habitats from isolates studied by others (Ropars et al., 2012, Jagielski et al., 2016, Sandoval-Denis et al., 2016) are also included. Finally, a table showing which Microascus , Scopulariopsis and Yunnania species are present in which habitat is constructed.…”

Section: Methodsmentioning

confidence: 99%

“…Although these two recent phylogenetic studies (Jagielski et al., 2016, Sandoval-Denis et al., 2016) make molecular identification of scopulariopsis-like isolates upon species level possible, the involved name changes can cause a lot of confusion. Commonly mentioned species from the indoor environment, like S. brumptii (now M. paisii ), S. fusca (now S. asperula ) and S. sphaerospora (now M. paisii ), are renamed.…”

Section: Introductionmentioning

confidence: 99%

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Scopulariopsisand scopulariopsis-like species from indoor environments

Woudenberg

Meijer

Houbraken

et al. 2017

Studies in Mycology

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Scopulariopsis-like species are often reported from the indoor environment, as well as from clinical samples. The lack of type isolates and thorough phylogenetic studies in the Microascaceae hampered the correct identification of these isolates. Based on recent phylogenetic studies, which resulted in multiple name changes, the aim is to molecularly identify the Scopulariopsis and scopulariopsis-like species which occur in the indoor environment and give an overview of the current species in these genera and their habitats. Strains from the CBS culture collection were supplemented with almost 80 indoor strains of which the internal transcribed spacer 1 and 2 and intervening 5.8S nrDNA (ITS), beta-tubulin (tub2) and translation elongation factor 1-alpha (tef1) gene regions were sequenced for phylogenetic inference. The multi-gene phylogenies recognise 33 Microascus species and 12 Scopulariopsis species and showed that the recently established genus Fuscoannellis, typified by Scopulariopsis carbonaria, should be synonymized with the genus Yunnania. Seven new Microascus species, four new Scopulariopsis species, and one new Yunnania species, are described, and a new name in Microascus and two new name combinations (one in Microascus, and one in Yunnania) are proposed. In the indoor environment 14 Microascus species and three Scopulariopsis species were found. Scopulariopsis brevicaulis (22 indoor isolates) and Microascus melanosporus (19 indoor isolates) are the most common indoor species, in number of isolates, followed by M. paisii (8 indoor isolates) and S. candida (7 indoor isolates). A genus phylogeny based on the ITS, tef1 and the large subunit 28S nrDNA (LSU) of the type or representative isolates of all here recognised species is provided depicting all species habitats. No correlation between phylogenetic relationship and habitat preference could be observed. Ten species which are found indoor are also found in relation with human-derived samples. A table showing recent name changes and a key to common species of Scopulariopsis and scopulariopsis-like genera found indoors is included.

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Fusarium and Other Opportunistic Hyaline Fungi

Zhang,

O'Donnell,

Wiederhold

et al. 2023

ClinMicroNow

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The opportunistic hyaline or lightly colored molds constitute a phylogenetically diverse group of common to rare fungi, some of which are only known to reproduce asexually while others possess sexual and asexual life cycles. Serologic procedures currently have little clinical utility in the diagnosis of uncommon hyaline opportunistic fungi. Identification of uncommon hyaline molds should always be evaluated considering the patient's immune status as well as the anatomic site of recovery and frequency of isolation. This chapter describes the salient colony and microscopic features of medically important species in the genus Fusarium and other select hyaline opportunists. As for Aspergillus and Candida , multilocus DNA typing systems have been developed to identify clinically and veterinary important isolates of Fusarium . The typical antifungal susceptibility profiles of Fusarium species are of relative resistance to most antifungal agents.

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Molecular taxonomy of scopulariopsis-like fungi with description of new clinical and environmental species

Cited by 27 publications

References 24 publications

Phylogeny and taxonomic revision of Microascaceae with emphasis on synnematous fungi

Phylogeny and taxonomic revision of Microascaceae with emphasis on synnematous fungi

Scopulariopsisand scopulariopsis-like species from indoor environments

Fusarium and Other Opportunistic Hyaline Fungi

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