Molecularly Imprinted Nanogels Possessing Dansylamide Interaction Sites for Controlling Protein Corona In Situ by Cloaking Intrinsic Human Serum Albumin

Abstract

Nanomaterials have become increasingly promising for biomedical applications owing to their specific biological characteristics. As drug delivery vehicles, nanomaterials have to circulate in the bloodstream to deliver the encapsulated components to the target tissues. Protein corona regulation is one of the promising approaches that gives stealth capability to avoid immune response. The aim of this study was to develop molecularly imprinted polymer nanogels (MIP-NGs) capable of protein corona regulation, using intrinsic human serum albumin (HSA) and with a functional monomer, dansylamide ethyl acrylamide (DAEAm), the dansylamide group serving as a ligand for HSA. The recognition capability of HSA for MIP-NGs was investigated by isothermal titration calorimetry (ITC). The affinity of the MIP-NGs prepared with DAEAm was then compared to that of the reference MIP-NGs prepared with pyrrolidyl acrylate developed in our previous study. Furthermore, we demonstrated that the concurrent use of these two different functional monomers for molecular imprinting was further effective to construct high-affinity recognition nanocavities for HSA and to form HSA-rich protein corona in the human plasma owing to the different interaction modes of the monomers. We believe that the molecular imprinting strategy developed through the use of ligand-based functional monomer is an effective strategy to create artificial molecular recognition materials.

In vivo stealthified molecularly imprinted polymer nanogels incorporated with gold nanoparticles for radiation therapy

“…The Au MIP-NGs circulate in blood for much longer times than the previously reported HSAimprinted nanogels 30 and Fc-domain imprinted nanogels. 31 This observation is possibly due to the Au MIP-NG particles being of suitable size for blood circulation, since the particles of the previously reported nanogels are much smaller (HSAimprinted nanogels: B35 nm, 30 Fc-domain imprinted nanogels: B16 nm 31 ) than the Au MIP-NG particles (B61 nm). The effect of particle size on blood circulation will be investigated in detail in the future.…”

“…In our previous study, a novel strategy to obtain stealth nanomaterials with immune escape capability against reticuloendothelial systems was developed based on the in situ control of the protein corona using intrinsic dysopsonic proteins. [30][31][32] In this strategy, the in situ regulation of the protein corona was achieved by conferring dysopsonic protein recognition capability on the nanomaterials. Molecularly imprinted polymer nanogels (MIP-NGs), which are promising materials for artificial antibodies, were prepared.…”

An electrochemical molecularly imprinted microfluidic paper-based chip for detection of inflammatory biomarkers IL-6 and PCT

“…Albumin recognition by MIP-NGs further inhibits the albumin dependence of cells on the uptake of nanogels and also reduces the uptake of cancer cells and macrophages. 5,6 Compared with biological antibodies, MIPs have the advantages of simple preparation, low cost and specific recognition.…”

Effect of Poly(Vinyl Alcohol) Concentration and Chain Length on Polymer Nanogel Formation in Aqueous Dispersion Polymerization

“…However, SDS is harmful for biological applications. Recently, we have successfully demonstrated the synthesis of PNIPAm-based nanogels via precipitation polymerization (without surfactants) under suitable conditions [34][35][36][37]. Furthermore, the polymer nanogels can recognize intrinsic dysopsonic proteins (serum albumin) and be employed as novel nanocarriers in drug delivery systems [34,38,39].…”