Molecularly Imprinted Polymers for Solid Phase Extraction

Qiao, Fang; Sun, Hanwen; Yan, Hongyuan; Row, Kyung Ho

doi:10.1365/s10337-006-0097-2

Cited by 184 publications

(108 citation statements)

References 159 publications

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“…Molecularly imprinted polymers (MIPs) find applications in many areas such as bio-mimetic sensors [11], antibody mimics [12] and catalysis [13]. They are very useful materials for isolation and preconcentration of low concentration analytes employing the solid phase extraction (SPE) technique [14][15][16]. Although many papers have been published concerning utilization of MIPs in pharmaceutical science [17][18][19], only a few were devoted to dopamine isolation [20][21][22][23][24].…”

mentioning

confidence: 99%

Dopamine-Imprinted Polymers: Template-Monomer Interactions, Analysis of Template Removal and Application to Solid Phase Extraction

Luliński

Maciejewska

Bamburowicz-Klimkowska

et al. 2007

Molecules

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A dopamine-imprinted polymer (MIP) was prepared in aqueous methanol solution at 60 o C by free-radical cross-linking polymerization of methacrylic acid in the presence of ethylene glycol dimethacrylate as the cross-linker and dopamine hydrochloride as the template molecule. Its ability to isolate dopamine was evaluated as the basis of a solid phase extraction procedure and compared with that of a non-imprinted polymer (NIP). The binding of dopamine was 84.1% and 29.1% for MIP and NIP, respectively. Various reported post-polymerization treatments to reduce template bleeding were examined. In our case the lowest bleeding was achieved after applying a combined procedure: continuous extraction in a Soxhlet apparatus (CE), followed by microwaveassisted extraction (ME) to a level of 0.061 μg/mL. A simplified model of the templatemonomer complexes allowed rationalization of monomer choice based on the heats of complex formation at a PM3 level of theory.

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mentioning

confidence: 99%

Dopamine-Imprinted Polymers: Template-Monomer Interactions, Analysis of Template Removal and Application to Solid Phase Extraction

Luliński

Maciejewska

Bamburowicz-Klimkowska

et al. 2007

Molecules

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“…Imprinted polymers are generated by polymerization of monomers and cross-linkers in the presence of a template [73]. The MIPs are expected to have high affinity for molecules resembling the template and can serve to extract such molecules (referred to as target molecules) from a complex mixture [74][75][76][77][78][79][80][81]. The interactions between the polymer and the target molecules are non-covalent interactions, and the affinity is largely based on the complementarity of the size, shape, electrostatics and hydrogen bonding of the MIP with the target molecule.…”

Section: Mammalian P450smentioning

confidence: 99%

Use of Chemical Auxiliaries to Control P450 Enzymes for Predictable Oxidations at Unactivated C-H Bonds of Substrates

Auclair

Polic

2015

Advances in Experimental Medicine and Biology

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Cytochrome P450 enzymes (P450s) have the ability to oxidize unactivated C-H bonds of substrates with remarkable regio-and stereoselectivity. Comparable selectivity for chemical oxidizing agents is typically difficult to achieve. Hence, there is an interest in exploiting P450s as potential biocatalysts. Despite their impressive attributes, the current use of P450s as biocatalysts is limited. While bacterial P450 enzymes typically show higher activity, they tend to be highly selective for one or a few substrates. On the other hand, mammalian P450s, especially the drugmetabolizing enzymes, display astonishing substrate promiscuity. However, product prediction continues to be challenging. This review discusses the use of small molecules for controlling P450 substrate specificity and product selectivity. The focus will be on two approaches in the area: (1) the use of decoy molecules, and (2) the application of substrate engineering to control oxidation by the enzyme.

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“…Molecularly imprinted polymers (MIPs) are polymers assembled in the presence of a template. [60][61][62][63][64][65][66][67][68] After removal of the template, MIPs tend to show selective affinity for molecules containing the template moiety. This occurs mainly through noncovalent interactions such as complementarity in size, shape, electrostatics and hydrogen bonding.…”

Section: Covalent Modifications To Extend the Substrate Promiscuity Amentioning

confidence: 99%

Controlling substrate specificity and product regio- and stereo-selectivities of P450 enzymes without mutagenesis

Polic

Auclair

2014

Bioorganic & Medicinal Chemistry

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P450 enzymes (P450s) are well known for their ability to oxidize unactivated C-H bonds with high regio-and stereoselectivity. Hence, there is emerging interest in exploiting P450s as potential biocatalysts. Although bacterial P450s typically show higher activity than their mammalian counterparts, they tend to be more substrate selective. Most drug-metabolizing P450s on the other hand, display remarkable substrate promiscuity, yet product prediction remains challenging. Protein engineering is one established strategy to overcome these issues. A less explored, yet promising alternative involves substrate engineering. This review discusses the use of small molecules for controlling the substrate specificity and product selectivity of P450s. The focus is on two approaches, one taking advantage of non-covalent decoy molecules, and the other involving covalent substrate modifications

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Molecularly Imprinted Polymers for Solid Phase Extraction

Cited by 184 publications

References 159 publications

Dopamine-Imprinted Polymers: Template-Monomer Interactions, Analysis of Template Removal and Application to Solid Phase Extraction

Dopamine-Imprinted Polymers: Template-Monomer Interactions, Analysis of Template Removal and Application to Solid Phase Extraction

Use of Chemical Auxiliaries to Control P450 Enzymes for Predictable Oxidations at Unactivated C-H Bonds of Substrates

Controlling substrate specificity and product regio- and stereo-selectivities of P450 enzymes without mutagenesis

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