Moleküle und biologische Mechanismen des Süß‐ und Umamigeschmacks

Behrens, Maik; Meyerhof, Wolfgang; Hellfritsch, Caroline; Hofmann, Thomas

doi:10.1002/ange.201002094

Cited by 12 publications

(3 citation statements)

References 195 publications

(236 reference statements)

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“…[2] Natural ribonucleoside monophosphates are not only nucleic acid building blocks but they are also employed as food additives due to their immunostimulant and taste enhancer activity. [3][4][5] Nucleoside analogues are widely used as antiviral and antitumour agents. [6,7] They can be incorporated in the viral or cellular DNA by DNA polymerases after phosphorylation by kinases, or they can inhibit the enzymes involved in the biosynthesis of DNA precursors.…”

Section: Introductionmentioning

confidence: 99%

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Immobilized Drosophila melanogaster Deoxyribonucleoside Kinase (DmdNK) as a High Performing Biocatalyst for the Synthesis of Purine Arabinonucleotides

et al. 2014

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Fruit fly (Drosophila melanogaster) deoxyribonucleoside kinase (DmdNK; EC: 2.7.1.145) was characterized for its substrate specificity towards natural and non-natural nucleosides, confirming its potential in the enzymatic synthesis of modified nucleotides. DmdNK was adsorbed on a solid ion exchange support (bearing primary amino groups) achieving an expressed activity > 98%. Upon cross-linking with aldehyde dextran, expressed activity was 30-40%. Both biocatalysts (adsorbed or cross-linked) were stable at pH 10 and room temperature for 24 h (about 70% of retained activity). The cross-linked DmdNK preparation was used for the preparative synthesis of arabinosyladenine monophosphate (araA-MP) and fludarabine monophosphate (FaraA-MP). Upon optimization of the reaction conditions (50 mM ammonium acetate, substrate/ATP ratio = 1:1.25, 2 mM MgCl 2 , 378C, pH 8) immobilized DmdNK afforded the title nucleotides with high conversion (> 90%), whereas with the soluble enzyme lower conversions were achieved (78-87%). Arabinosyladenine monophosphate was isolated in 95% yield and high purity (96.5%).

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Section: Introductionmentioning

confidence: 99%

“…Natural ribonucleoside monophosphates are not only nucleic acid building blocks but they are also employed as food additives due to their immunostimulant and taste enhancer activity 3–5…”

Section: Introductionmentioning

confidence: 99%

Immobilized Drosophila melanogaster Deoxyribonucleoside Kinase (DmdNK) as a High Performing Biocatalyst for the Synthesis of Purine Arabinonucleotides

et al. 2014

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“…Some natural ribonucleoside monophosphates (i. e. IMP, GMP) are used as food additives due to their immunostimulant and taste enhancer activity . 2’‐Deoxynucleoside‐5’‐monophosphates (5’‐dNMPs) are building blocks for the production of 2’‐deoxynucleoside‐5’‐triphosphates (5’‐dNTPs) which are the substrates for polymerase chain reaction (PCR) …”

Section: Introductionmentioning

confidence: 99%

Immobilization of Deoxyadenosine Kinase fromDictyostelium discoideum(DddAK) and Its Application in the 5’-Phosphorylation of Arabinosyladenine and Arabinosyl-2-fluoroadenine

et al. 2017

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This manuscript is dedicated to the memory of Prof. Jure Piškur Deoxyadenosine kinase from Dictyostelium discoideum (DddAK) phosphorylates its natural substrate (2'-deoxyadenosine, dAdo) as well as the arabinosyladenine analogues vidarabine (araA) and fludarabine (F-araA) to their corresponding 5'-monophosphates. DddAK has been here immobilized by ionic interaction on an aminated epoxy-functionalized support (Sepabeads TM EC-EP), and cross-linked with oxidized dextran. The final activity recovery was 33-42 %, depending on the protein loading. Immobilization enhanced the stability of DddAK at pH 10 and, to a lesser extent, at 45 8C. Phosphorylation of dAdo, araA and F-araA catalyzed by immobilized DddAK was always nearly quantitative in less than 12 hours. Fludarabine monophosphate was synthesized from F-araA (95 % conversion, 20 g/L) using immobilized DddAK in fully aqueous medium, thus showing that this biocatalyst could be used for developing a preparative phosphorylation process greener and more efficient than POCl 3 -based phosphorylation.[a] Dr.

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Optochemische Genetik

2011

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Transmembranrezeptoren ermöglichen die Kommunikation zwischen Zellen und ihrer Umgebung. Die Stimuli, die diese Rezeptoren aktivieren, treten in Form von Änderungen der Ligandenkonzentration (z. B. Hormone oder Neurotransmitter), der Temperatur, des Drucks (z. B. Schallwellen oder Berührungen), des Transmembranpotentials oder der Lichtintensität auf. Viele Transmembranrezeptoren sind heutzutage in atomarer Auflösung charakterisiert, und unser Verständnis ihrer funktionellen Eigenschaften hat sich in vergangenen Jahren deutlich verbessert. Infolgedessen können diese hochentwickelten molekularen Maschinen umprogrammiert und gegenüber unnatürlichen Reizen sensibilisiert werden. In diesem Aufsatz zeigen wir, wie spannungs‐ und ligandengesteuerte Ionenkanäle mit synthetischen Lichtschaltern ausgestattet werden können. Die daraus resultierenden künstlichen Lichtrezeptoren können genutzt werden, um neurale Aktivität mit einer außergewöhnlichen zeitlichen und räumlichen Präzision optisch zu kontrollieren. Sie sind bereits erfolgreich in lebenden Systemen eingesetzt worden und könnten in Zukunft bei der Wiederherstellung des Sehprozesses und der optischen Kontrolle anderer Sinneswahrnehmungen Anwendung finden. Die Kombination von synthetischen Photoschaltern und Rezeptorproteinen erweitert das Feld der Optogenetik. Darüber hinaus verleiht sie der chemischen Genetik eine neue Dimension, weswegen wir vorschlagen, diesen Forschungsansatz “optochemische Genetik” zu nennen.

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Moleküle und biologische Mechanismen des Süß‐ und Umamigeschmacks

Cited by 12 publications

References 195 publications

Immobilized Drosophila melanogaster Deoxyribonucleoside Kinase (DmdNK) as a High Performing Biocatalyst for the Synthesis of Purine Arabinonucleotides

Immobilized Drosophila melanogaster Deoxyribonucleoside Kinase (DmdNK) as a High Performing Biocatalyst for the Synthesis of Purine Arabinonucleotides

Immobilization of Deoxyadenosine Kinase fromDictyostelium discoideum(DddAK) and Its Application in the 5’-Phosphorylation of Arabinosyladenine and Arabinosyl-2-fluoroadenine

Optochemische Genetik

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