Monitoring and Control of Bioproducts from Conception to Production in Real‐time Using an Optical Biosensor

Bracewell, Daniel G.; Brown, Robert A.; Gill, Anita; Hoare, Mike

doi:10.1002/1618-2863(200107)1:1<25::aid-elsc25>3.0.co;2-#

Cited by 4 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hänel et al provide a good comparison of SPR and RIF sensors based on the interaction between thrombine and thrombine inhibitor [35]. Bracewell et al [37] have shown that a SPR sensor with automated sample handling only needs 30 s to perform an analysis whereas an ELISA assay needs 3-24 h. In addition, SPR and RIF are not limited to quantification of proteins. It is also possible to determine the kinetics of the interactions used for the analysis (antigen-antibody interactions).…”

Section: Affinity Sensors (Spr Rif and Rm)contrasting

confidence: 41%

Optical sensor systems for bioprocess monitoring

2003

View full text Add to dashboard Cite

Bioreactors are closed systems in which microorganisms can be cultivated under defined, controllable conditions that can be optimized with regard to viability, reproducibility, and product-oriented productivity. To drive the biochemical reaction network of the biological system through the desired reaction optimally, the complex interactions of the overall system must be understood and controlled. Optical sensors which encompass all analytical methods based on interactions of light with matter are efficient tools to obtain this information. Optical sensors generally offer the advantages of noninvasive, nondestructive, continuous, and simultaneous multianalyte monitoring. However, at this time, no general optical detection system has been developed. Since modern bioprocesses are extremely complex and differ from process to process (e.g., fungal antibiotic production versus mammalian cell cultivation), appropriate analytical systems must be set up from different basic modules, designed to meet the special demands of each particular process. In this minireview, some new applications in bioprocess monitoring of the following optical sensing principles will be discussed: UV spectroscopy, IR spectroscopy, Raman spectroscopy, fluorescence spectroscopy, pulsed terahertz spectroscopy (PTS), optical biosensors, in situ microscope, surface plasmon resonance (SPR), and reflectometric interference spectroscopy (RIF).

show abstract

Section: Affinity Sensors (Spr Rif and Rm)contrasting

confidence: 41%

Optical sensor systems for bioprocess monitoring

2003

View full text Add to dashboard Cite

show abstract

“…Another important aspect is that the technique uses a ligand receptor interaction to make a biospecific measurement not possible with HPLC or electrophoresis measurements. A more detailed explanation of the rationale behind the use of the technique can be obtained from [18]. In this work we examine the impact the ability to perform real-time measurements of a bioproduct throughput a process can have for monitoring and control.…”

Section: Introductionsupporting

confidence: 39%

Addressing a whole bioprocess in real-time using an optical biosensor-formation, recovery and purification of antibody fragments from a recombinant E. coli host

Bracewell

Brown

Hoare

2004

Bioprocess Biosyst Eng

Self Cite

View full text Add to dashboard Cite

The use of biosensor technology is described to address in real-time the production and subsequent purification of a bioactive recombinant protein product. The product, D1.3 Fv antibody fragment, was expressed in Escherichia coli and purified via two process routes, one for extracellular and one for intracellular product material. The cells were harvested by centrifugation in a solid bowl CARR Powerfuge and stored at -70 degrees C. Clarification of the supernatant was performed by depth filtration, followed by affinity chromatography for final purification of the extracellular product. To purify the intracellular product the harvested cells were resuspended and homogenised. Removal of debris in the CARR Powerfuge was followed by depth filtration and affinity chromatography. In this work we have shown the rapid determination of bioactive product levels, and the impact this has on improved accountability and confidence is demonstrated in process mass balances on the product using the data acquired during process operation.

show abstract

Survey of the year 2001 commercial optical biosensor literature

Rich

Myszka

2002

J of Molecular Recognition

102

View full text Add to dashboard Cite

We have assembled references of 700 articles published in 2001 that describe work performed using commercially available optical biosensors. To illustrate the technology's diversity, the citation list is divided into reviews, methods and specific applications, as well as instrument type. We noted marked improvements in the utilization of biosensors and the presentation of kinetic data over previous years. These advances reflect a maturing of the technology, which has become a standard method for characterizing biomolecular interactions.

show abstract

Monitoring and Control of Bioproducts from Conception to Production in Real‐time Using an Optical Biosensor

Cited by 4 publications

References 0 publications

Optical sensor systems for bioprocess monitoring

Optical sensor systems for bioprocess monitoring

Addressing a whole bioprocess in real-time using an optical biosensor-formation, recovery and purification of antibody fragments from a recombinant E. coli host

Survey of the year 2001 commercial optical biosensor literature

Contact Info

Product

Resources

About