Monitoring of cefepime in urine by micellar electrokinetic capillary chromatography with ultraviolet detection and liquid chromatography coupled to mass spectrometry

Kummer, Mirjam; Šestáková, Nela; Theurillat, Regula; Huynh‐Do, Uyen; Endimiani, Andrea; Sendi, Parham; Thormann, Wolfgang

doi:10.1002/jssc.201800763

Cited by 10 publications

(7 citation statements)

References 32 publications

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“…To prove the analytical ability of the proposed OT‐CEC assay, the urinary samples spiked with the three test drugs were analyzed. The results are displayed in Figure .…”

Section: Resultsmentioning

confidence: 99%

Separation of antipyretic analgesics by open tubular capillary electrochromatography with homopolymer coatings

Liu

Qiao

Zhang

et al. 2019

J of Separation Science

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This study developed an open‐tubular capillary electrochromatography protocol for the analysis of antipyretic analgesic drugs, which used a multifunctional homopolymer as coating. A controlled/living radical polymerization strategy was adopted to obtain poly(N‐acryloxysuccinimide) with a tunable chain‐length. The homopolymer coating enhanced the separation performance by contributing to the hydrophobic and hydrogen‐bonding interactions between the analytes and the homopolymer. The effect of polymer chain‐length and buffer pH and concentration on the separation efficiency was evaluated. In this approach, baseline separation of the three test drugs was achieved within 15 min. The repeatability of the prepared homopolymer coating was investigated, with the relative standard deviations < 2.88% observed in intra‐ and interday runs. Good linearity in the 5–800 µM range (R2 ≥ 0.998) demonstrates that accurate quantitative analysis of real samples was achieved. Moreover, the proposed assay was used to quantify the three drugs (aminopyrine, 4‐aminoantipyrine, and phenacetin) in urine samples, achieving recovery rates between 92.1 and 108.7%. This promising methodology may be used for the analysis of drugs in real bio‐samples and for the development of unique homopolymer coatings for open‐tubular capillary electrochromatography systems.

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“…To prove the analytical ability of the proposed OT‐CEC assay, the urinary samples spiked with the three test drugs were analyzed. The results are displayed in Figure .…”

Section: Resultsmentioning

confidence: 99%

Separation of antipyretic analgesics by open tubular capillary electrochromatography with homopolymer coatings

Liu

Qiao

Zhang

et al. 2019

J of Separation Science

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“…Recently, innovative detection methods have also been used, such as capacitively coupled contactless conductivity detection (C 4 D) or procedure design gradient detection (PGD) [19]. Table 2 provides conditions for the determination of antibiotics using electromigration techniques [20][21][22][23][24].…”

Section: Analytical Methods In the Determination And Identification O...mentioning

confidence: 99%

Bioanalytics in In Vitro and In Vivo Transformation of Biologically Active Compounds for the Needs of Biomedical Diagnostics

Szultka‐Młyńska

Pauter

Walczak-Skierska

et al. 2022

Handbook of Bioanalytics

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Drugs are active substances which trigger various biological reactions. Generally, the medicine is to improve our health condition, but it is not always so. Having used the drug for a long time or in inadequate doses, various undesirable side effects may occur. Additionally, newly produced drugs may be poisonous if we fail to examine their metabolic biotransformation in the living organism. For this reason, drugs are tested using in vivo and in vitro research in order to evaluate their biological activity and toxicity. In vivo tests concern the research on living animals such as rodents or monkeys, while in vitro tests use isolated enzymes within the microsomal fraction, cells, tissues, and whole organs of animals or people (tests with the biological material taken from a patient). Both

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“…ABZSO and ABZSO 2 serum levels were determined by LC‐MS using an EC 125/4 Nucleodur C18 Isis 5 μm HPLC column (Macherey ‐ Nagel) together with a HP series 1100 (Hewlett Packard, Waldbronn, Germany) coupled to an Agilent 6120 Single Quadrupole LC‐MS System with an APCI/ESI interface (Agilent Technologies, Santa Clara, CA). This setup with gradient elution was previously employed to monitor the antibiotic drug cefepime in extracts of human serum and plasma [34] and in urine [35]. For analysis of ABZSO and ABZSO 2 , isocratic elution was used with a mobile phase composed of 20 mM formic acid (63 %) and methanol (37 %) and the flow rate was 0.7 mL/min.…”

Section: Methodsmentioning

confidence: 99%

Aspects of albendazole metabolism in western lowland gorillas (Gorilla gorilla gorilla) compared to humans and other species assessed by HPLC, LC‐MS, and chiral electrokinetic chromatography

Theurillat

Stirnimann

Wenker

et al. 2021

Separation Science Plus

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The benzimidazole anthelmintic drug albendazole becomes metabolized to chiral albendazole sulfoxide, albendazole sulfone, albendazole 2-aminosulfone, and other metabolites. HPLC with UV absorbance and fluorescence detection, LC-MS, and chiral electrokinetic chromatography were used to analyze albendazole metabolites in extracts of western lowland gorilla (Gorilla gorilla gorilla) plasma of patients with alveolar echinococcosis and the data are compared to those obtained with human patient samples and to those reported for other species in the literature. The data revealed that the albendazole sulfoxide to albendazole sulfone concentration ratio in gorilla plasma is significantly smaller compared to that in samples of men, rats, dogs, and cattle. It is, however, similar to what was observed in sheep and goats. The (+)-albendazole sulfoxide to (−)-albendazole sulfoxide enantiomeric ratio in gorilla blood determined by chiral electrokinetic chromatography was found to be >1 which is comparable to what was observed in humans, dogs, sheep, goats, and cattle but different to mice and rats. Furthermore, the electrokinetic chromatography data suggest that small amounts of albendazole 2-aminosulfone may be present in plasma of gorillas and humans.The data gathered with the various separation-based assays illustrate the value of technology featuring different analyte separation and detection principles. This is the first account describing aspects of the albendazole metabolism in gorillas.

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Monitoring of cefepime in urine by micellar electrokinetic capillary chromatography with ultraviolet detection and liquid chromatography coupled to mass spectrometry

Cited by 10 publications

References 32 publications

Separation of antipyretic analgesics by open tubular capillary electrochromatography with homopolymer coatings

Separation of antipyretic analgesics by open tubular capillary electrochromatography with homopolymer coatings

Bioanalytics in In Vitro and In Vivo Transformation of Biologically Active Compounds for the Needs of Biomedical Diagnostics

Aspects of albendazole metabolism in western lowland gorillas (Gorilla gorilla gorilla) compared to humans and other species assessed by HPLC, LC‐MS, and chiral electrokinetic chromatography

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