Monitoring Poultry Farms for Salmonella by Drag-Swab Sampling and Antigen-Capture Immunoassay

Mallinson, E. T.; Tate, C. R.; Miller, Russell G.; Bennett, Barbara S.; Russek‐Cohen, Estelle

doi:10.2307/1591145

Cited by 58 publications

(20 citation statements)

References 17 publications

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“…1,12 Floor and feeder samplings were performed after the animals were removed and the pens disinfected, using drag swab sets. 21 The isolated Salmonella strains were serotyped according to Popoff and Le Minor. 27 Resistance to each antibiotic was determined by the Kirby-Bauer disk susceptibility test 28 using commercially prepared filter paper disks (Oxoid Ltd., Hampshire, UK), each impregnated with a specified amount of an antibiotic.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial Resistance ofSalmonellaSerotypes Isolated from Slaughter-Age Pigs and Environmental Samples

Oliveira

Carvalho

Fernandes

et al. 2002

Microbial Drug Resistance

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The aim of this study was to determine the antimicrobial resistance patterns of Salmonella strains isolated from slaughter-age pigs and environmental samples collected at modern swine raising facilities in Brazil. Seventeen isolates of six serotypes of Salmonella enterica subsp. enterica were isolated out of 1,026 collected samples: Salmonella Typhimurium (1), Salmonella Agona (5), Salmonella Sandiego (5), Salmonella Rissen (1), Salmonella Senftenberg (4), and Salmonella Javiana (1). Resistance patterns were determined to extendedspectrum penicillin (ampicillin), broad-spectrum cephalosporins (cefotaxime and ceftriaxone), aminoglycosides (streptomycin, neomycin, gentamicin, amikacin, and tobramycin), narrow-spectrum quinolone (nalidixic acid), broad-spectrum quinolone (ciprofloxacin and norfloxacin), tetracycline, trimethoprim, and chloramphenicol. Antimicrobial resistance patterns varied among serotypes, but isolates from a single serotype consistently showed the same resistance profile. All isolates were resistant to tetracycline, streptomycin, and nalidixic acid. One isolate, Salmonella Rissen, was also resistant to cefotaxime and tobramycin. All serotypes were susceptible to ceftriaxone, norfloxacin, ciprofloxacin, ampicillin, gentamicin, and chloramphenicol. The high resistance to tetracycline and streptomycin may be linked to their common use as therapeutic drugs on the tested farms. No relation was seen between nalidixic acid and fluoroquinolone resistance. 407

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Section: Methodsmentioning

confidence: 99%

Antimicrobial Resistance ofSalmonellaSerotypes Isolated from Slaughter-Age Pigs and Environmental Samples

Oliveira

Carvalho

Fernandes

et al. 2002

Microbial Drug Resistance

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“…Swabs from manure and egg belts in implicated poultry houses were examined for S. enteritidis by using previously described methods [14,15]. In poultry houses where environmental tests recovered S. enteritidis, 300 hens were serologically tested with the use of a rapid plate test for pullorum antigen.…”

Section: Methodsmentioning

confidence: 99%

A comparison ofSalmonella enteritidisphage types from egg-associated outbreaks and implicated laying flocks

et al. 1993

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SUMMARYInfections due to Salmonella enteritidis are increasing worldwide. In the United States, between 1985 and 1989, 78 % of the S. enteritidis outbreaks ill which a food vehicle was identified implicated a food containing raw or lightly cooked shell eggs.Under a US Department of Agriculture regulation published in 1990, eggs implicated in human food-borne S. enteritidis outbreaks were traced back to the source flock. The flock environment and the internal organs of a sample of hens were tested for S. enteritidis. We compared the S. enteritidis phage types of isolates from 18 human, egg-associated outbreaks and the 15 flocks implicated through traceback of these outbreaks. The predominant human outbreak phage type was recovered from the environment in 100 % of implicated flocks and from the internal organs of hens in 88 % of implicated flocks we tested. The results support the use of phage typing as a tool to identify flocks involved in human S. enteritidis outbreaks.

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“…However, for these programs to be completely effective requires the ability to rapidly screen breeder flocks, broiler flocks, layer flocks and their corresponding environments. Of the current methods recommended, direct litter sampling and the drag-swab method are the most widely accepted for whole house contamination evaluation (Bhatia et al, 1980;Kingston, 1981;Mallinson et al 1989;Opara et al, 1992;Caldwell et al, 1994). However, both methods suffer from sample size limitations and because these methods also require physical collection, increasing sample size requires more labor and adds to the cost of monitoring.…”

Section: Introductionmentioning

confidence: 99%

Recovery of a marker strain ofSalmonella typhimuriumin litter and aerosols from isolation rooms containing infected chickens

Kwon

Woodward

Corrier

et al. 2000

Journal of Environmental Science and Health, Part B

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Screening of poultry flocks for foodborne pathogen Salmonella contamination is critical for Salmonella control in preharvest stages of poultry production. In this study, two sampling methods (litter and air filter) were compared for detection of S. typhimurium from experimentally infected chicks some of which had received either a probiotic competitive exclusion culture or transfer of cecal contents from salmonellae-free adult birds. At 4, 9, and 11 days after inoculation, S. typhimurium samples were enumerated by selective plating. For both types of sampling, the control birds yielded the greatest levels of environmental contamination followed by the samples from the probiotic inoculated birds with the birds receiving the cecal transfer culture having the lowest levels of contamination. Although the two sampling methods responded in a similar fashion, detection sensitivity needs to be increased for air filter sampling.

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Monitoring Poultry Farms for Salmonella by Drag-Swab Sampling and Antigen-Capture Immunoassay

Cited by 58 publications

References 17 publications

Antimicrobial Resistance ofSalmonellaSerotypes Isolated from Slaughter-Age Pigs and Environmental Samples

Antimicrobial Resistance ofSalmonellaSerotypes Isolated from Slaughter-Age Pigs and Environmental Samples

A comparison ofSalmonella enteritidisphage types from egg-associated outbreaks and implicated laying flocks

Recovery of a marker strain ofSalmonella typhimuriumin litter and aerosols from isolation rooms containing infected chickens

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