Monitoring the maturation process of a dental microcosm biofilm using the Quantitative Light-induced Fluorescence-Digital (QLF-D)

Kim, Young Seok; Lee, Eun Song; Kwon, Ho Keun; Kim, Baek Il

doi:10.1016/j.jdent.2014.03.006

Cited by 97 publications

(80 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…6,7,12 Recently, some in vitro studies showed that the red fluorescence of biofilm grown with sucrose exposure was correlated with maturity and with some cariogenic properties of this biofilm. 13,21 However, the authors did not evaluate the fluorescence of the biofilm formed for the time with no sucrose exposure. 13,21 Therefore, it would not be possible to infer if the fluorescence was due to maturity or because of cariogenic characteristics.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Is the red fluorescence of dental plaque related to its cariogenicity?

et al. 2014

View full text Add to dashboard Cite

Abstract. It has been speculated that the red fluorescence emitted by dental plaque could be related to its cariogenicity. To test this hypothesis, we designed this crossover in situ study, with two experimental phases of 14 days each. Seventeen volunteers, wearing a palatal appliance with bovine enamel blocks, were instructed to drip a 20% sucrose solution (experimental group) or purified water (control group) onto the enamel blocks eight times daily. The specimens were removed after 4, 7, 10, and 14 days, and the red fluorescence of dental plaque formed on the enamel blocks was assessed using a quantitative light-induced fluorescence device. After the plaque removal, surface and cross-sectional microhardness tests were performed to assess the mineral loss. The comparisons were made by a multilevel linear regression analysis. We observed a significant increase in the red fluorescence of the dental plaque after longer periods of formation, but this trend was verified in both groups. The mineral loss assessed by the microhardness techniques, contrariwise, showed a significant increase only in the experimental group. In conclusion, the red fluorescence emitted by the dental plaque indicates a mature biofilm, but this fact is not necessarily associated with its cariogenicity. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

show abstract

Section: Discussionmentioning

confidence: 99%

“…13,21 However, the authors did not evaluate the fluorescence of the biofilm formed for the time with no sucrose exposure. 13,21 Therefore, it would not be possible to infer if the fluorescence was due to maturity or because of cariogenic characteristics.…”

Section: Discussionmentioning

confidence: 99%

Is the red fluorescence of dental plaque related to its cariogenicity?

et al. 2014

View full text Add to dashboard Cite

show abstract

“…In our previous study, we monitored the maturation of microcosm biofilms for 10 days and found that the red fluorescence appeared at day 3 and gradually increased until day 7. 15 However, there is still a lack of literature examining the relation between the red fluorescence and the ecological changes in microcosm biofilms during maturation.…”

Section: Introductionmentioning

confidence: 99%

Ecological changes in oral microcosm biofilm during maturation

et al. 2016

Self Cite

View full text Add to dashboard Cite

Abstract. The aim of this study was to evaluate the ecological changes in the biofilm at different stages of maturation using 16S rDNA gene amplicon sequencing and to identify correlations between red/green (R/G) fluorescence ratio and ecological changes. An oral microcosm biofilm was initiated from the saliva of a single donor and grown anaerobically for up to 10 days in basal medium mucin. Quantitative light-induced fluorescence analysis was shown that the R/G ratio of the biofilm increased consistently, but the slope rapidly decreased after six days. The bacterial compositions of 10 species also consistently changed over time. However, there was no significant correlation between each bacteria and red fluorescence. The monitoring of the maturation process of oral microcosm biofilm over 10 days revealed that the R/G ratio and the bacterial composition within biofilm consistently changed. Therefore, the R/G fluorescence ratio of biofilm may be related with its ecological change rather than specific bacteria. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

show abstract

“…The Red over Green ratio of pixel values is generally used to identify red fluorescent plaque. Therefore, previous work performed on QLF-images [13,9] used the Red over Green pixel intensities' ratio instead of using the Red channel's pixel intensity values only. The Green channel helps to distinguish plaque from gingiva, since they have slightly different pixel values in Green channel of RGB representation.…”

Section: Influence Of Multi-channel Representationmentioning

confidence: 99%

“…Existing computer programs developed for this goal have several drawbacks which limit efficiency of QLF-images assessment. They require that the images must have been captured under the fixed circumstances such as camera geometry, focal distance and ambient light conditions [9], which is hard to achieve under clinical settings.…”

Section: Introductionmentioning

confidence: 99%

Artificial Neural Networks and Machine Learning – ICANN 2017

2017

Lecture Notes in Computer Science

View full text Add to dashboard Cite

Abstract. Images are an important data source for diagnosis and treatment of oral diseases. The manual classification of images may lead to misdiagnosis or mistreatment due to subjective errors. In this paper an image classification model based on Convolutional Neural Network is applied to Quantitative Light-induced Fluorescence images. The deep neural network outperforms other state of the art shallow classification models in predicting labels derived from three different dental plaque assessment scores. The model directly benefits from multi-channel representation of the images resulting in improved performance when, besides the Red colour channel, additional Green and Blue colour channels are used.

show abstract

Monitoring the maturation process of a dental microcosm biofilm using the Quantitative Light-induced Fluorescence-Digital (QLF-D)

Cited by 97 publications

References 20 publications

Is the red fluorescence of dental plaque related to its cariogenicity?

Is the red fluorescence of dental plaque related to its cariogenicity?

Ecological changes in oral microcosm biofilm during maturation

Artificial Neural Networks and Machine Learning – ICANN 2017

Contact Info

Product

Resources

About