Monoclonal Antibodies Against the Whitefly-Transmitted Tomato Yellow Leaf Curl Virus and Their Application in Virus Detection

Wu, Jiajing; Shang, Haili; Shen, Qingtang; Zhou, Xueping

doi:10.1016/s2095-3119(12)60010-0

Cited by 23 publications

(15 citation statements)

References 26 publications

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“…Samples were treated in loading buffer (50 mM of Tris–HCl, pH 6.8, 10% glycerol, 0.1% bromophenol blue, 1% mercaptoethanol and 2% SDS) and separated by 10% SDS‐PAGE gel. The details for western blot analysis were described in Whitfield et al The anti‐CP MAb (3E10) was used at a 1:5000 dilution . The anti‐His antibody (Transgen Biotech, Beijing, China) was used at a 1:2500 dilution.…”

Section: Methodsmentioning

confidence: 99%

Expression and functional characterisation of a soluble form of Tomato yellow leaf curl virus coat protein

Wang

Wei

et al. 2014

Pest Management Science

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Section: Methodsmentioning

confidence: 99%

Expression and functional characterisation of a soluble form of Tomato yellow leaf curl virus coat protein

Wang

Wei

et al. 2014

Pest Management Science

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“…The purified virions were stained with 2% (w/v, g mL -1 ) phosphotungstic acid and examined with an electron microscope (JEM−1200 EX, JEOL Ltd., Tokyo, Japan). Preparation of MAb against TYLCV was performed using the protocol as described previously [13]. …”

Section: Methodsmentioning

confidence: 99%

“…However, the reported PAbs are only suitable for Western blot analysis rather than ELISA detection in field samples [12]. Recently, recombinant coat protein (CP) was used as an immunogen to produce MAbs and PAbs against TYLCV, and a TAS-ELISA was developed for detecting five begomoviruses [13]. In this study, six MAbs against TYLCV were produced using the hybridoma technology and two serological methods, dot enzyme-linked immunosorbent assay (dot-ELISA) and direct tissue blot immunoassay (DTBIA) using the most sensitive MAb 1C4 were successfully developed for detecting TYLCV in field plant and insect vector samples.…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive serological methods for detecting tomato yellow leaf curl virus in tomato plants and whiteflies

Jiao

Zhou

Liu

et al. 2013

Virol J

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BackgroundTomato yellow leaf curl virus (TYLCV) is a member of the genus Begomovirus in the family Geminiviridae, which causes severe losses in tomato production in tropic and subtropic regions.MethodsThe purified TYLCV virions were used as the immunogen to produce monoclonal antibodies (MAbs) using the hybridoma technology. MAb-based dot enzyme-linked immunosorbent assay (dot-ELISA) and direct tissue blot immunoassay (DTBIA) were developed for sensitive, simple, and rapid detection of TYLCV in field tomato and whitefly (Bemisia tabaci) samples collected from TYLCV prevalent provinces in China.ResultsUsing the hybridoma technology, six murine MAbs (1C4, 8D10, 6E3, 2F2, 3F4 and 4G3) against TYLCV were prepared. Using the MAb 1C4, dot-ELISA and DTBIA were then established for detecting TYLCV in field tomato and whitefly samples collected from TYLCV prevalent provinces in China. The dot-ELISA could detect TYLCV in infected tissue crude extract diluted at 1:5,120 (w/v, g mL-1), and in viruliferous whitefly homogenate diluted at 1:128 (individual whitefly/μL), respectively. Field tomato samples (n=487) and whitefly samples (n=110) from TYLCV prevalent districts in China were screened for the presence of TYLCV using the two developed methods, and the results were further confirmed by PCR and nucleotide sequencing. The survey revealed that TYLCV is widespread on tomato plants in Zhejiang, Shandong and Henan provinces in China.ConclusionsThe developed dot-ELISA is very suitable for the routine detection of TYLCV in field tomato and whitefly samples, and the DTBIA is more suitable for the routine detection of TYLCV in large-scale tomato plant samples collected from TYLCV prevalent areas.

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“…Tomatoes infected by the virus exhibit different forms of symptoms such as stunting and flower abortion, curling of the leaflet margins, yellowing of young leaves, inferior quality of the fruits and decreased yields. The symptoms on the plant also depends on many factors including, the selected cultivar, management options adopted, and environmental conditions (Moriones and Navas-Castillo, 2000;Wu et al, 2012;Chen et al, 2013;Rakibet al, 2011;Pan et al, 2012) Strategies for managing whiteflies and TYLCV Management of TYLCV is challenging and costly. A combination of management options is necessary to successfully manage the disease and limit losses.…”

Section: Tomato Yellow Leaf Curl Virus (Tylcv)mentioning

confidence: 99%

Economic Productivity and Profitability Analysis for Whiteflies and Tomato Yellow Leaf Curl Virus (TYLCV) Management Options

Fonsah¹,

Diffie²,

Srinivansan³

et al. 2018

JAES

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The United States is the second largest producer of tomatoes (Lycopersicon esculentum Mill.) in the world. In the U.S. tomato is produced for fresh and processed markets, respectively, and both markets contribute over $2 billion in annual farm cash receipts (Wells, 2012). Tomato yellow leaf curl virus (TYLCV), transmitted by whiteflies, is a major threat to tomato production in the United States and around the world (Polston et al. 1999, Moriones andNavas-Castillo, 2000;Lefeuvre et al., 2010). The spread of the virus in the field is directly correlated to increase in B. tabaci populations (Rakib et al., 2011;Adi et al., 2012). Infected plants display severe symptoms, are less vigorous, and produce fruits with reduced market value. It is common to experience yield losses of up to 100% in affected fields (Rakib et al., 2011;Pan et al., 2012;, Srinivasan et al. 2012). There are several management tactics available. However, there is no single most effective "silver bullet" management tactic to manage whiteflies and TYLCV. Therefore, it is vital to integrate several tactics to suppress pest and virus incidences and to boost yields. This study investigates the economic productivity and profitability of combining management options such as insecticides, reflective mulch, and virus-resistant cultivars for whiteflies and TYLCV management. The economic models adopted for this study include farm enterprise budgeting, sensitivity analysis and break-even analysis. Results show that total pre-harvest variable cost was $4,200/ac and the expected net return was $1,958/ac was attainable 50% of the time.

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Monoclonal Antibodies Against the Whitefly-Transmitted Tomato Yellow Leaf Curl Virus and Their Application in Virus Detection

Cited by 23 publications

References 26 publications

Expression and functional characterisation of a soluble form of Tomato yellow leaf curl virus coat protein

Expression and functional characterisation of a soluble form of Tomato yellow leaf curl virus coat protein

Highly sensitive serological methods for detecting tomato yellow leaf curl virus in tomato plants and whiteflies

Economic Productivity and Profitability Analysis for Whiteflies and Tomato Yellow Leaf Curl Virus (TYLCV) Management Options

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