Monoclonal Antibodies to a Penaeid Shrimp Parvovirus, Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV)

Poulos, Bonnie T.; Lightner, Donald V.; Trumper, Bronwen; Bonami, Jean Robert

doi:10.1577/1548-8667(1994)006<0149:mataps>2.3.co;2

Cited by 18 publications

(8 citation statements)

References 9 publications

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“…These methods are more sensitive than IHHNV detection by histology and are therefore used widely for virus detection. Monoclonal antibodies (mAbs) have been developed for the detection of IHHNV (Poulos, Lightner, Trumper, & Bonami, 1994) but have not been used as much as in situ hybridization and dot blot assay for disease diagnosis. Monoclonal antibodies (mAbs) have been developed for the detection of IHHNV (Poulos, Lightner, Trumper, & Bonami, 1994) but have not been used as much as in situ hybridization and dot blot assay for disease diagnosis.…”

Section: Histopathology and Virus Detectionmentioning

confidence: 99%

Biology, Genome Organization, and Evolution of Parvoviruses in Marine Shrimp

Dhar

Robles‐Sikisaka

Saksmerprome

et al. 2014

Advances in Virus Research

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Section: Histopathology and Virus Detectionmentioning

confidence: 99%

Biology, Genome Organization, and Evolution of Parvoviruses in Marine Shrimp

Dhar

Robles‐Sikisaka

Saksmerprome

et al. 2014

Advances in Virus Research

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“…For this reason, new highly sensitive diagnostic tools, such as molecular probes and monoclonal antibodies, were recently developed for some shrimp virus diseases, i.e. IHHN (infectious hypodermal and haematopoietic necrosis) (Man et al 1993, Poulos et al 1994a), MBV-type (monodon baculovirus) (Poulos et al 199413) and BP-type (baculovirus penaei) diseases (Bruce et al 1993, Bonami et al 1995.…”

Section: Introductionmentioning

confidence: 99%

Partial cloning of the genome of an unusual shrimp parvovirus (HPV):use of gene probes in disease diagnosis

Mari¹,

Lightner²,

Poulos³

et al. 1995

Dis. Aquat. Org.

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The DNA genome of the hepatopancreatic parvovirus (HPV) of penaeid s h n m p was partially cloned The largest insert obtained, from the clone OF44, was 2 3 k b p In size and characterized by mapping w~t h restnction enzymes It was used to construct 2 different DIG-11 dUTP labelled probes, which appeared specific to the virus, without any cross-reactivity w~t h uninfected tissues or with a second s h n m p parvovirus, infectious hypodermal and haematopoietlc necrosis virus (IHHNV) In sltu hybridization on paraffin sections of the hepatopancreas of infected and uninfected shrimp showed the probes react with Infected tlssue and not with umnfected tissue In positive hepatopancreas, the probes produced a strong labelling of infected nuclei, especially of the nucleolus and the karyoplasm peripheric to the intranuclear inclusion body Intranuclear inclusion bodies, which are the principal diagnostic feature of HPV infections by histology, also reacted with the probes, but less intensely In heavily infected hepatopancreas, cell d e b r~s reacted strongly wlth the probes, as did the microvilli on the surface of eplthelial cells

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“…Although monoclonal antibodies to IHHNV have been reported, no antibody-based antigen detection methods for IHHNV have been successfully developed (Poulos et al, 1994;Lightner and Redman, 1998a;OIE, 2006b). However, molecular techniques using dot-blot and in-situ hybridization (ISH) tests for IHHNV have been developed and a number of methods and commercial products using these methods are available (OIE, 2006b).…”

Section: Antibody-based and Ish Diagnostic Methods For Ihhnvmentioning

confidence: 99%

Virus diseases of farmed shrimp in the Western Hemisphere (the Americas): A review

Lightner

2011

Journal of Invertebrate Pathology

314

192

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Penaeid shrimp aquaculture is an important industry in the Americas, and the industry is based almost entirely on the culture of the Pacific White Shrimp, Litopenaeus vannamei. Western Hemisphere shrimp farmers in 14 countries in 2004 produced more than 200,000 metric tons of shrimp, generated more than $2 billion in revenue, and employed more than 500,000 people. Disease has had a major impact on shrimp aquaculture in the Americas since it became a significant commercial entity in the 1970s. Diseases due to viruses, rickettsial-like bacteria, true bacteria, protozoa, and fungi have emerged as major diseases of farmed shrimp in the region. Many of the bacterial, fungal and protozoan caused diseases are managed using improved culture practices, routine sanitation, and the use of chemotherapeutics. However, the virus diseases have been far more problematic to manage and they have been responsible for the most costly epizootics. Examples include the Taura syndrome pandemic that began in 1991-1992 when the disease emerged in Ecuador, and the subsequent White Spot Disease pandemic that followed its introduction to Central America from Asia in 1999. Because of their socioeconomic significance to shrimp farming, seven of the nine crustacean diseases listed by the World Animal Organization (OIE) are virus diseases of shrimp. Of the seven virus diseases of penaeid shrimp, five are native to the Americas or have become enzootic following their introduction. The shrimp virus diseases in the Americas are increasingly being managed by exclusion using a combination of biosecurity and the practice of culturing domesticated specific pathogen-free (SPF) stocks or specific pathogen-resistant (SPR) stocks. Despite the significant challenges posed by disease, the shrimp farming industry of the Americas has responded to the challenges posed by disease and it has developed methods to manage its diseases and mature into a sustainable industry.

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Monoclonal Antibodies to a Penaeid Shrimp Parvovirus, Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV)

Cited by 18 publications

References 9 publications

Biology, Genome Organization, and Evolution of Parvoviruses in Marine Shrimp

Biology, Genome Organization, and Evolution of Parvoviruses in Marine Shrimp

Partial cloning of the genome of an unusual shrimp parvovirus (HPV):use of gene probes in disease diagnosis

Virus diseases of farmed shrimp in the Western Hemisphere (the Americas): A review

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