Monoclonal antibody-based ELISA for the quantification of nitrofuran metabolite 3-amino-2-oxazolidinone in tissues using a simplified sample preparation

Diblíková, I; Cooper, Kevin M.; Kennedy, D. G.; Fránek, Milan

doi:10.1016/j.aca.2005.03.039

Cited by 90 publications

(96 citation statements)

References 14 publications

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“…For nitrofuran metabolites, haptens were always designed by derivatising the metabolites with 3-CBA or 4-CBA [6,7,[9][10][11][12][13][14][15]. Although this strategy was able to generate antibodies with high specificity and sensitivity, it's believed that there are some other strategies to further improve the antibody characteristics.…”

Section: Design and Synthesis Of Haptensmentioning

confidence: 99%

“…Cheng et al [8] tried to introduce a 6-methoxyhexanoic acid spacer on the 5' position of phenyl ring of NPAOZ and used it as a new immunizing hapten. This hapten possessed the -NO 2 group and therefore the corresponding antibody showed higher sensitivity and specificity against NPAOZ in ELISA, compared with the antibody generated with CPAMOZ [6][7][8]. However, the hapten was difficult to synthesize.…”

Section: Design and Synthesis Of Haptensmentioning

confidence: 99%

“…The first enzyme-linked immunosorbent assay (ELISA) capable of detecting the furazolidone metabolite (AOZ) was developed by Cooper et al [6] under the support of a multi-national EU research project "FoodBRAND" [2]. Since then, immunoassays for the determination of AOZ [7][8][9], SEM [10][11][12], AHD [13,14] and AMOZ [15] were reported in succession.…”

Section: Introductionmentioning

confidence: 99%

“…NPAOZ, NASEM, NPAHD and NPAMOZ) [6][7][8][9][10][11][12][13][14][15]. In most studies, nitrofuran metabolites were derivatised with 3-carboxybenzaldehyle (3-CBA) or 4-carboxybenzaldehyle (4-CBA) to form immunizing haptens [6,7,[9][10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

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Novel hapten synthesis for antibody production and development of an enzyme-linked immunosorbent assay for determination of furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ)

Shen

Sun

et al. 2013

Talanta

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Publisher rights This is the author's version of a work that was accepted for publication in Talanta. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Talanta, VOL 103, 01/2012 General rights Copyright for the publications made accessible via the Queen's University Belfast Research Portal is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights. Cite this article as: Zhen-Lin Xu, Yu-Dong Shen, Yuan-Ming Sun, Katrina Campbell, Yuan-Xin Tian, Shi-Wei Zhang, Hong-Tao Lei and Yue-Ming Jiang, Novel hapten synthesis for antibody production and development of an enzyme-linked immunosorbent assay for determination of furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ), Talanta, http://dx.doi.org/10. 1016/j.talanta.2012.10.059 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractA heterologous competitive indirect enzyme-linked immunosorbent assay (ciELISA) for the determination of the furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) was developed. AMOZ was derivatised with 2-(4-formylphenoxy)acetic acid or 2-(3-formylphenoxy)acetic acid to obtain two novel immunizing haptens. The ability of these haptens in producing specific polyclonal antibodies against the nitrophenyl derivative of AMOZ (NPAMOZ) was compared with that of traditional immunizing haptens (derivatised AMOZ with 3-carboxybenzaldehyle or 4-carboxybenzaldehyle). The results indicated that the novel immunizing haptens were able to produce antibodies with almost a two-fold improvement in sensitivity of the ciELISA for NPAMOZ in comparison with the existing antibody based ELISAs. The differences in sensitivity were explained by the molecular modeling of the lowest energy conformations of NPAMOZ and the haptens. Another novel hapten, derivatised AMOZ with 2-oxoacetic acid, was synthesized and used as a heterologous coating hapten. The results showed that this strategy of using only a partial structure of the target molecule as the coating hapten was able to obtain a two to three-fold improvement in sensitivity. This study provided a modern approach for the development of an immunoassay with improved sensitivity for the metabolites of nitrofuran antibiotics.

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Section: Design and Synthesis Of Haptensmentioning

confidence: 99%

Section: Design and Synthesis Of Haptensmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Novel hapten synthesis for antibody production and development of an enzyme-linked immunosorbent assay for determination of furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ)

Shen

Sun

et al. 2013

Talanta

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show abstract

“…To date a range of methods for the determination of nitrofuran metabolites and CAP residues in foodstuff have been developed. Independent enzyme linked immunosorbent assays (ELISAs) are widely used for screening purposes for each nitrofuran metabolite Diblikova et al, 2005;Cheng et al, 2009;Li et al, 2009) and CAP (Fodey et al, 2007;Liu et al, 2014;Guo et al, 2015) thereby requiring five ELISA assays to detect all targets. Commercial screening tests are available for honey from Abraxis, Europroxima, Randox Food Diagnostics, R-Biopharm, Romer Labs and Tecna for nitrofurans detection and from Europroxima, Randox Food Diagnostics, R-Biopharm, Tecna and Bioo Scientific for CAP detection.…”

Section: Introductionmentioning

confidence: 99%

Feasibility of a novel multispot nanoarray for antibiotic screening in honey

McNamee

Rosar²,

Persic³

et al. 2017

Food Additives & Contaminants: Part A

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Rapid chemical reduction synthesis of copper nanoclusters with blue fluorescence for highly sensitive detection of furazolidone

Guo,

Wang,

Zhang

et al. 2024

Luminescence

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Tannic acid (TA), as a stabilizing agent, was successfully utilized to establish blue‐emitting copper nanoclusters (TA‐Cu NCs) on the basis of a facile chemical reduction preparation method. Characterization results proved successful synthesis of TA‐Cu NCs with uniform size and excellent stability. TA‐Cu NCs exhibited a blue emission wavelength at 431 nm when excited at 364 nm. Interestingly, the as‐prepared TA‐Cu NCs were selectively quenched by furazolidone based on static quenching. In addition, this analysis platform for furazolidone detection had an excellent linear range from 0.5 to 120 μM with a detection limit of 0.074 μM (S/N = 3). Furthermore, the accuracy of this sensing method was successfully confirmed by detecting furazolidone in bovine serum samples, indicating that TA‐Cu NCs had bright application prospects.

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Monoclonal antibody-based ELISA for the quantification of nitrofuran metabolite 3-amino-2-oxazolidinone in tissues using a simplified sample preparation

Cited by 90 publications

References 14 publications

Novel hapten synthesis for antibody production and development of an enzyme-linked immunosorbent assay for determination of furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ)

Novel hapten synthesis for antibody production and development of an enzyme-linked immunosorbent assay for determination of furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ)

Feasibility of a novel multispot nanoarray for antibiotic screening in honey

Rapid chemical reduction synthesis of copper nanoclusters with blue fluorescence for highly sensitive detection of furazolidone

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