Monoclonal rat anti-major histocompatibility complex antibodies display specificity for rat, mouse, and human target cells.

Abstract

24 monoclonal rat antibodies are described that are reactive with determinants encoded by the major histocompatibility complex (MHC) of the rat. These hybridoma antibodies were derived by fusing mutant mouse myeloma cells to spleen cells from Lewis rats immunized with allogeneic Brown Norway cells. All 24 antibodies are cytotoxic for both Brown Norway target cells and target cells from the appropriate MHC congenic rats. Pattern of cytotoxicity and hemagglutination strongly suggest reactivity against class I (K or D equivalent) rat MHC determinants. Cytotoxic cross-reactivity patterns were generated for each monoclonal antibody on a panel of rat and mouse lymphoid cells and human peripheral T lymphocytes. A high degree of interspecies cross-reactivity was noted with approximately one-half of the antibodies positive on human and/or mouse target cells. 11 antibodies recognized polymorphic determinants in the mouse, and, by using target cells from MHC congenic mouse strains, it was shown that these determinants are encoded by genes within the H-2 complex. Finally, by considering the overall reactivity patterns of these monclonal antibodies on all target cells, one can show that these 24 antibodies represent a minimum of 14 antibody specificities.

Analysis of T cell receptor ? chains in the rat: I. Allelic polymorphism of V?8.2 is not a predisposing genetic factor in susceptibility to experimental allergic encephalomyelitis

“…PBL were stained using either the RT1 .A' specific monoclonal antibody (mAb), I169 (Smilek et al, 1980), or the RT1.A" specific mAb, MAC 30 (Butcher, 1987). Briefly, blood cells were collected in Hanks balanced salt solution (HBSS) containing 1000 units/ml sodium heparin, washed 2X in cold PBS containing 0.2% sodium azide and 2% BSA (PBS-BSA), and red blood cells were removed by lysis in 0.2% Trizma base/0.8% ammonium chloride, pH 7.2.…”

The contribution of L3T4+ T cells to lymphoproliferation and autoantibody production in MRL-lpr/lpr mice.

“…The hybridoma D4.69 (control rat Ig) produces an IgG mAb with specificity for the RT1-N.A. antigen and shows no activity against murine cells (13). RA3 secretes an IgM mAb that reacts with an antigen (B220) present on B cells and pre-B cells that is expressed on the mutant T cells of lpr mice (7) .…”

Suppression of liver transplant rejection by anti-donor MHC antibodies via depletion of donor immunogenic dendritic cells

“…The portal area was defined by a presence of the bile ducts depicted by type IV collagen. Since infiltrated recipient cells densely accumulated mainly in the portal and the hepatic vein areas ( 24 ), the sinusoidal area could be easily depicted by the immunostaining for recipient MHCI (I169.1) ( 25 ) and type IV collagen. Photomicrographs with a 4× objective were taken and the proportions were estimated by image analysis.…”