Monocrotophos pesticide modulates the expression of sexual differentiation genes and causes phenotypic feminization in zebrafish (Danio rerio)

Zhang, Xiaona; Gao, Lei; Yang, Kuo-Pin; Tian, Hua; Wang, Wei; Ru, Shaoguo

doi:10.1016/j.cbpc.2012.09.004

Cited by 28 publications

(15 citation statements)

References 49 publications

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“…The prolonged exposure of monocrotophos on Clarias gariepinus has a significant reduction effect on weight gain and specific growth rate ratio (Yaji and Auta 2007). Monocrotophos exert toxic effects on the reproductive system of teleost fishes by changing the balance of sex steroid hormones (Wang et al 2015), modulates the expression of sexual differentiation genes, and causes phenotypic feminization in zebrafish (Zhang et al 2013a) and disruption of hypothalamic-pituitary-thyroid axis in male goldfish, Carassius auratus (Zhang et al b).…”

Section: Introductionmentioning

confidence: 98%

Developmental toxic effects of monocrotophos, an organophosphorous pesticide, on zebrafish (Danio rerio) embryos

Pamanji

Bethu

Yashwanth

et al. 2015

Environ Sci Pollut Res

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The present study examined the response of zebrafish embryos exposed to different concentrations (10, 20, 30, 40, 50, and 60 mg/L) of monocrotophos under static conditions for 96 h. We found that mortality had occurred within 48 h at all test concentrations, later insignificant mortality was observed. Monocrotophos (MCP) can be rated as moderately toxic to the Zebrafish embryos with a 96-h median lethal concentration (LC50) of 37.44 ± 3.32 mg/L. In contrast, it greatly affected the development of zebrafish embryos by inducing several developmental abnormalities like pericardial edema, altered heart development, spinal and vertebral anomalies in a concentration-dependent manner. A significant percent reduction in length by 9-48% and heart beats by 18-51% was observed in hatchlings exposed to LC10 and LC50 concentrations at 96 h when compared to controls. The process of looping formation of heart at embryonic stage was greatly affected by the LC50 concentration of MCP. The neurotoxic potentiality of MCP was assessed by using a marker enzyme, acetylcholinesterase in both in vitro and in vivo experiments. MCP was found to be the most potent inhibitor of AChE in vitro with an IC50 value of 4.3 × 10(-4) M. The whole-body AChE enzyme activity in vivo was significantly inhibited during the exposure tenure with the maximum inhibition of 62% at 24 h.

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Section: Introductionmentioning

confidence: 98%

Developmental toxic effects of monocrotophos, an organophosphorous pesticide, on zebrafish (Danio rerio) embryos

Pamanji

Bethu

Yashwanth

et al. 2015

Environ Sci Pollut Res

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“…Although a number of fish models are used for toxicity screening of aquatic environment, the teleost cyprinid, zebrafish (Danio rerio) is the most popular choice because of its prominence as a toxicological model (Hill et al 2005;Sipes et al 2011). A few studies have been carried out on the different effects of MCP on zebrafish such as the estrogenic and feminizing effects of MCP (Zhang et al 2013) and cortisol-mediated stress responses (Zhang et al 2015). In a recent study, different concentrations of MCP were used to evaluate the developmental response of zebrafish embryos (Pamanji et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Induction of DNA damage in the peripheral blood of zebrafish (Danio rerio) by an agricultural organophosphate pesticide, monocrotophos

et al. 2018

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The use of organophosphate pesticides (OPs) in agricultural practices in India has gained popularity due to their broad spectrum, low cost and high potency. Monocrotophos (an OP) which is a systemic and contact broad-spectrum cholinesterase inhibitor is being widely used in India. However, its use entails the damage to other non-target organisms such as fish. In the present study, zebrafish (Danio rerio) were exposed to various doses of monocrotophos (0.125, 0.625 and 1.25 lL/L) and genotoxicity studies were carried out employing the comet assay and micronucleus test in the peripheral blood of these fish after 24, 48 and 72 h of exposure. Significant DNA damage in the form of % tail DNA and micronuclei was observed in all the treated fish compared to the control. Both % tail DNA and micronuclei frequency were found to increase significantly as the concentration increased. DNA damage was also observed at all the time intervals for all treatments except in the 0.125 lL/L-treated group. A positive correlation was also observed between the comet assay and micronucleus test. The study thus suggests the use of zebrafish as an experimental model to study the genotoxic effects of agricultural pesticides using the comet assay and micronucleus test.

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“…Xenoestrogens are structurally similar to 17β -estradiol (E2), such as 17α -ethinylestradiol (EE2), which exert their estrogenic effects on gonadal differentiation by mimicking the actions of endogenous estrogens and thereby inducing phenotypic feminization (Andersen et al, 2003;Kuhl et al, 2005). The phenotypic feminization in Banded Gourami fish induced by chlorpyrifos toxicity observed in our study is in accordance with earlier studies in fishes exposed to different EDCs (Holbech et al, 2006;Marchand et al, 2010;Tillitt et al, 2010;Tian et al, 2012;Zhang et al, 2013).…”

Section: Effects Of Chlorpyrifos On Testessupporting

confidence: 90%

Effects of insecticides on aquatic ecosystems in Bangladesh

Sumon

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Monocrotophos pesticide modulates the expression of sexual differentiation genes and causes phenotypic feminization in zebrafish (Danio rerio)

Cited by 28 publications

References 49 publications

Developmental toxic effects of monocrotophos, an organophosphorous pesticide, on zebrafish (Danio rerio) embryos

Developmental toxic effects of monocrotophos, an organophosphorous pesticide, on zebrafish (Danio rerio) embryos

Induction of DNA damage in the peripheral blood of zebrafish (Danio rerio) by an agricultural organophosphate pesticide, monocrotophos

Effects of insecticides on aquatic ecosystems in Bangladesh

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