Monogalactosyldiacylglycerol and digalactosyldiacylglycerol role, physical states, applications and biomimetic monolayer films

Hoyo, Javier; Guaus, E.; Torrent‐Burgués, J.

doi:10.1140/epje/i2016-16039-0

Cited by 28 publications

(23 citation statements)

References 66 publications

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“…The shape of the π-A curve with MGDG monolayer spread at the air-water interface was shown in Supplementary Materials Figure S1 . The collapse pressure for MGDG monolayer film was recorded to be 41 mN/m, and was coincidence with the previous reported 42–43 mN/m [ 22 ]. According to the values presented by Hoyo et al, MGDG presents a liquid-condensed (LC) phase at low surface pressure (10–15 mM/m), and the solid (S) state formation at 33 mN/m.…”

Section: Resultssupporting

confidence: 90%

Recombinant Lipase from Gibberella zeae Exhibits Broad Substrate Specificity: A Comparative Study on Emulsified and Monomolecular Substrate

Wang

Zhang

Zhao

et al. 2017

IJMS

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Using the classical emulsified system and the monomolecular film technique, the substrate specificity of recombinant Gibberella zeae lipase (rGZEL) that originates from Gibberella zeae was characterized in detail. Under the emulsified reaction system, both phospholipase and glycolipid hydrolytic activities were observed, except for the predominant lipase activity. The optimum conditions for different activity exhibition were also determined. Compared with its lipase activity, a little higher ratio of glycolipid hydrolytic activity (0.06) than phospholipase activity (0.02) was found. rGZEL preferred medium chain-length triglycerides, while lower activity was found for the longer-chain triglyceride. Using the monomolecular film technique, we found that the preference order of rGZEL to different phospholipids was 1,2-diacyl-sn-glycero-3-phospho-l-serine (PS) > 1,2-dioleoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt (PG) > 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) > l-α-phosphatidylinositol (PI) > cardiolipin (CL) > 3-sn-phosphatidic acid sodium salt (PA) > l-α-phosphatidylethanolamine (PE), while no hydrolytic activity was detected for sphingomyelin (SM). Moreover, rGZEL showed higher galactolipase activity on 1,2-distearoyimonoglactosylglyceride (MGDG). A kinetic study on the stereo- and regioselectivity of rGZEL was also performed by using three pairs of pseudodiglyceride enantiomers (DDGs). rGZEL presented higher preference for distal DDG enantiomers than adjacent ester groups, however, no hydrolytic activity to the sn-2 position of diglyceride analogs was found. Furthermore, rGZEL preferred the R configuration of DDG enantiomers. Molecular docking results were in concordance with in vitro tests.

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Section: Resultssupporting

confidence: 90%

Recombinant Lipase from Gibberella zeae Exhibits Broad Substrate Specificity: A Comparative Study on Emulsified and Monomolecular Substrate

Wang

Zhang

Zhao

et al. 2017

IJMS

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“…The comparison of the 2D-contour plots of the free energy calculated with different renormalization parameter values suggests that ρ − ε s coupling leads to the persistence of the LE phase even at the later stage of the linear slope regime beyond a transition midpoint surface pressure in the two-phase coexistence region. This may be closely related to the appearance of an isothermal compressibility plateau as often previously observed in lipid monolayers [19,20,25].…”

Section: Introductionsupporting

confidence: 70%

“…Response functions are closely related to equilibrium fluctuations of order parameters in the system. In this respect, the appearance of a compressibility plateau [13,19,20,25,33] in the LE-LC phase-coexistence region means that molecular density fluctuations remains almost constant and therefore that they do not decay easily even across at the midpoint of the transition. This indicates that ρ − ε s coupling plays a significant role for the persistence of equilibrium molecular density fluctuations or for the delay of transition progress in the nonhorizontal linear π increase regime of the isotherm.…”

Section: Discussionmentioning

confidence: 99%

Emergence of a linear slope region of the isotherm in the first-order liquid-expanded–liquid-condensed phase transition in Langmuir monolayers

Hatta

Nihei

2019

Phys. Rev. E

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A nonhorizontal slope in the isotherm has been observed in the two-phase coexisting region of the firstorder liquid-expanded (LE)-liquid-condensed (LC) phase transition in Langmuir monolayers for many decades. We show that the simple analysis of a phenomenological Landau free energy involving the coupling-energy contributions of molecular lateral density (ρ ) with spontaneous collective chain tilt (θ) and two-dimensional strain (ε s ) inside the LC domain enables one to understand the origin of a nonhorizontal straight-line slope in the LE-LC phase coexistence region of the isotherm. The presence of ρ − ε s coupling must be essential for the appearance of the straight-line shape of a nonhorizontal plateau in the isotherm. Moreover, it is found from the comparison of the two-dimensional contour plots of the free energy that an LE phase may persist significantly even at the later stage of the straight-line regime beyond a transition midpoint surface pressure in the presence of this coupling. The persistence of the LE phase may lead to the delay of transition progress as manifested more clearly by the appearance of a compressibility plateau in the coexistence region that indicates the existence of persistent equilibrium density fluctuations in the monolayer.

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“…The use of Langmuir and Langmuir-Blodgett (LB) techniques allows a higher control on the membrane physical state and structure compared with vesicle fusion technique [46,47]. The physical states derived from the surface-pressure isotherms are confirmed by the zones with different height observed in AFM images, which confirms the presence of UQ in both physical states.…”

Section: Introductionmentioning

confidence: 78%

Tuning ubiquinone position in biomimetic monolayer membranes

2017

Self Cite

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Artificial lipid bilayers have been extensively studied as models that mimic natural membranes (biomimetic membranes). Several attempts of biomimetic membranes inserting ubiquinone (UQ) have been performed to enlighten which the position of UQ in the lipid layer is, although obtaining contradictory results. In this work, pure components (DPPC and UQ) and DPPC:UQ mixtures have been studied using surface pressure-area isotherms and Langmuir-Blodgett (LB) films of the same compounds have been transferred onto solid substrates being topographically characterized on mica using atomic force microscopy and electrochemically on indium tin oxide slides. DPPC:UQ mixtures present less solid-like physical state than pure DPPC indicating a higher-order degree for the latter. UQ influences considerably DPPC during the fluid state, but it is mainly expelled after the phase transition at [Formula: see text] 26 mN·m^-1 for the 5:1 ratio and at [Formula: see text] 21 mN·m^-1 for lower UQ content. The thermodynamic studies confirm the stability of the DPPC:UQ mixtures before that event, although presenting a non-ideal behaviour. The results indicate that UQ position can be tuned by means of the surface pressure applied to obtain LB films and the UQ initial content. The UQ positions in the biomimetic membrane are distinguished by their formal potential: UQ located in "diving" position with the UQ placed in the DPPC matrix in direct contact with the electrode surface ( -0.04±0.02 V), inserted between lipid chains without contact to the substrate ( 0.00±0.01 V) and parallel to the substrate, above the lipid chains ( 0.09±0.02 V).

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Monogalactosyldiacylglycerol and digalactosyldiacylglycerol role, physical states, applications and biomimetic monolayer films

Cited by 28 publications

References 66 publications

Recombinant Lipase from Gibberella zeae Exhibits Broad Substrate Specificity: A Comparative Study on Emulsified and Monomolecular Substrate

Recombinant Lipase from Gibberella zeae Exhibits Broad Substrate Specificity: A Comparative Study on Emulsified and Monomolecular Substrate

Emergence of a linear slope region of the isotherm in the first-order liquid-expanded–liquid-condensed phase transition in Langmuir monolayers

Tuning ubiquinone position in biomimetic monolayer membranes

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