Monomolecular film behavior of tetraether lipids from a thermoacidophilic archaebacterium at the air/water interface

Archaebacterial bipolar tetraether lipids: Physico-chemical and membrane properties

“…BTL can form plain or S-layer-supported monolayers (Dote et al, 1990;Schuster et al, 1998Schuster et al, , 2003, which are useful tools for studying membrane-bound proteins and transport phenomena as well as for developing new technological applications. These planar monolayers are stable.…”

Langmuir films stability phenomenon of glycerol dialkyl nonitol tetraether at the air-water interface for variations spreading time

“…showed that Langmuir film GDNT area per molecule ranging from 14 nm 2 for the first compression-decompression cycle and 11 nm 2 at the seventh cycle. Dote et al [13] and Elferinck et al [14] reported that GDNT molecules are organised in an upright standing configuration with a molecular area of 0.82 nm 2 in the monomolecular films. In monolayer experiments with GDNT from Sulfolobus acidocaldarius, De Rosa [15] found molecular areas between 1.0 nm 2 and 1.6 nm 2 .…”

“…Floating monolayer stability is an important parameter in obtaining very high-quality Langmuir-Blodgett films. Although, efforts were made to obtain high-quality Langmuir-Blodget films of tetraether lipids [7,[9][10][11][13][14][15][16]. Langmuir-Blodget of tetraether lipid films depends on many parameters such as substrate surface properties (hydrophobic, hydrophilic, aminosilanised) [10], temperature, sub-phase, etc.…”

Biomimetic interfaces based on S-layer proteins, lipid membranes and functional biomolecules

“…However, a disadvantage is that tetraether lipids may adopt instead an upright conformation (figure 3b (1)), an unstable so-called U-shaped (bent) conformation at the air/water interphase where both hydrophilic head groups interact with the aqueous subphase (figure 3b (2)) [64,67,68]. Thus, films comprising tetraether lipids may not be stable in terms of maintaining the adjusted surface pressure over time as permanent rearrangement of the lipid molecules occurs [62,69,70]. Owing to their amphiphilic nature, ether-and phospholipids can nicely be self-assembled at the air/water interphase with the hydrocarbon chains pointing towards the air and the polar head groups facing the aqueous phase (figure 3b(3)) [71,72].…”