Monooxygenation, cytochrome P4501A1 and P4501A1-mRNA in rat liver slices exposed to beta-naphthoflavone and dexamethasone in vitro

Evaluation of the precision-cut liver and lung slice systems for the study of induction of CYP1, epoxide hydrolase and glutathione S-transferase activities

“…Exposure of the slices, however, to benzo[a]pyrene led to a marked, increase in activity, the highest rise occurring after a 24-h incubation, similar to observations made in vivo following treatment of rats with this compound (Iwasaki et al, 1986). A rise in this activity in rat slices has already been reported following incubation with other CYP1A1 inducers such as ␤-naphthoflavone (Müller et al, 1996), TCDD (Drahushuk et al, 1996) and Aroclor 1254 (Price et al, 2004). In one study, where different incubation time points were employed, all longer than 24 h, level of induction by TCDD was found to increase with time of incubation being highest after 96 h of incubation (Drahushuk et al, 1999), in contrast to the present study where maximum induction was noted after a 24-h exposure to benzo[a]pyrene.…”