Morinda officinalis polysaccharides improve meat quality by reducing oxidative damage in chickens suffering from tibial dyschondroplasia

Protective effects of chlorogenic acid on the meat quality of oxidatively stressed broilers revealed by integrated metabolomics and antioxidant analysis

“…5,8 In addition, oxidative stress accelerates postmortem glycolysis, thereby lowering the meat pH. Broiler chickens suffering from oxidative stress yielded breast muscles with inferior quality traits including increased drip loss, cooking loss and shear force, and lower pH, 25,29,36–38 and similar results were obtained in this study. Dietary CGA supplementation decreased the drip loss, cooking loss, and L *, but increased the pH of breast muscles in oxidatively stressed broilers, similarly to its effects on pigs and lambs.…”

“…Metabolomic profiling of breast muscles was performed as described previously. 25 The samples (50 mg) were mixed with methanol/water (ice-cold, 1000 μL, 70%, v/v) and internal standard (2-chlorophenylalanine, 5 μL, 1 μg mL −1 ), homogenized at 30 Hz for 3 min, vortexed for 1 min, and then centrifuged at 12 000 g for 10 min at 4 °C. After centrifugation, the supernatant was filtered using a 0.22 μm filter membrane and stored at −20 °C overnight.…”

“…24 In addition, mass spectrometry (MS)–based metabolomics has been an effective approach to screen metabolites for the biomarkers related to meat quality and antioxidant capacity in broilers. 25,26 These studies highlighted the effectiveness of metabolomics for the quality evaluation of meat; therefore, an approach integrating metabolomics and molecular biology should advance the understanding of the protective effects of CGA on meat quality.…”

Αξιολόγηση Της Ποιότητα, Ασφάλειας Και Αυθεντικότητας Του Κρέατος Με Ταχείες Μεταβολομικές Μεθόδους

“…Metabolic pathway analysis is an effective method to analyze the correlation between metabolites and can reveal the roles of different metabolites in meat (Huang et al, 2020) during storage and distribution. In our case, where meat was stored aerobically, Carnobacteria could be in spoil oxidizing conditions, which favour the pyruvate metabolism through the TCA cycle (Afzal et al, 2010).…”

“…Θα πρέπει να διερευνηθούν επίσης τα μεταβολικά μονοπάτια που ακολουθούνται κατά τη συντήρηση, ούτως ώστε να μπορέσουμε να ελέγξουμε. Η ανάλυση της μεταβολικής οδού (pathway analysis) είναι μια αποτελεσματική μέθοδος για την ανάλυση της συσχέτισης μεταξύ των μεταβολιτών καθώς επίσης ο ρόλος αυτών στο κρέας κατά τη συντήρηση και διανομή(Huang et al, 2020) Συγκριτική απεικόνιση μεθόδων που εφαρμόζονται στην ανάλυση των τροφίμων…”