There are little data about antioxidants’ status responses to early thermal conditioning (TC) on broiler chickens. Therefore, the present study was conducted to investigate the different time ages of thermal conditioning on antioxidants responses and the growth rate of broiler chicks. A total of two hundred forty‐one‐day‐old male broiler chicks (Cobb 500) weighed on average 51.5 ± 0.5g were randomly distributed into four equal groups (60 chicks each), and chicks of each group were ranked in five replicates. The first group reared under the ambient temperature, while the second, third and fourth groups (TC3, TC5 and TC7) were subjected to early‐age thermal conditioning at 39°C for 6 h on the third, fifth and seventh day of age respectively. Broilers were fed ad‐libitum, and drinking water was a free choice during the experimental period. At the end of the experimental period that lasted 5 weeks, all experimental groups were exposed to heat challenge at 36°C for 6 h. Early‐age thermal conditioning did not affect growth performance. Plasma corticosterone elevation in TC5 (as a post‐challenge response) was the lowest among the experimental groups. Hepatic malondialdehyde significantly increased in TC5 and TC7 groups both at the post‐conditioning stage and at the end of the experimental period. Hepatic glutathione, glutathione S‐transferases, catalase and superoxide dismutase significantly decreased by early‐age thermal conditioning compared with non‐conditioned broilers. Microscopic examination of the liver sections from broilers chickens in TC5 and TC7 groups showed all the basic features of normal liver tissue, while the control and TC3 groups showed few necrotic areas. It could be concluded that early‐age thermal conditioning at 39°C for 6 h on the fifth day of age could improve the antioxidant defence system of broilers without any adverse effects on growth performance.