Morphoanatomy and changes in antioxidant defense associated with the natural ventilation system of micropropagated Lippia dulcis plantlets

Rocha, Tainá Teixeira; Araújo, Diene Xavier; Silva, André Maciel da; Oliveira, Jean Paulo Vitor de; Carvalho, Alexandre Alves de; Gavilanes, Manuel Losada; Bertolucci, Suzan Kelly Vilela; Alves, Eduardo; Pinto, José Eduardo Brasil Pereira

doi:10.1007/s11240-022-02364-8

Cited by 12 publications

(8 citation statements)

References 43 publications

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“…The results obtained for LAR (0.014 cm 2 mg -1 ) suggest that Lippia dulcis plantlets grown in MS had a larger leaf area that was useful for carrying out photosynthesis, which contributed to gains in LDW shown in Table 2. Rocha et al (2022) found that the highest LAR contributed to higher dry weight averages in Lippia dulcis under in vitro conditions. According to Benincasa (2003), the leaves are the center for the production of photosynthates (dry weight), and the rest of the plant depends on the export of this material through the leaves.…”

Section: Growth Analysismentioning

confidence: 86%

“…Some studies have already demonstrated that different in vitro conditions can affect the growth of Lippia species. Rocha et al (2022) reported that light intensity and light quality influenced plantlets propagation, providing better conditions for the acclimatization phase. Lazzarini et al (2019a) observed the influence of the explant type and natural ventilation system and concluded that explants with a pair of leaves and a ventilation system with four membranes promoted the greatest growth, leaf area, and carvacrol and thymol contents in L. gracilis.…”

Section: Introductionmentioning

confidence: 99%

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Establishment and clonal propagation of Lippia dulcis Trevir. through in vitro single node cultures

Rocha

Araújo

Assis

et al. 2023

PCC&M

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Lippia dulcis Trevir. (Verbenaceae) is a species with medicinal potential that is used as a tranquilizer and to control diabetes in the western region of Pará, Brazil. The objective of this study was to determine the establishment and clonal propagation of Lippia dulcis through single nodal segments grown in different concentrations of salts in a MS (Murashige and Skoog) medium (MS, MS/2, MS/4) and determine the best period for subculturing the species. Nodal segments cultivated in the MS medium showed a higher survival percentage and better development than those grown in more dilute media (MS/2, MS/4). The concentration of the salts affected the dry weight gain of some plant parts. Plantlets cultivated in the MS medium obtained better leaf dry weight and total leaf area than plantlets cultivated in half-strength MS. The half-strength MS medium proved to be most effective for root length growth and root dry weight gainin vitro. The growth curve showed that day 30 was the time for subculturing the species. Starting with only one nodal segment, 3125 plantlets were obtained after sixth months of cultivation at a multiplication rate of five. The plantlets of Lippia dulcis were removed from the flasks and acclimatized with success in a greenhouse.

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Section: Growth Analysismentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Establishment and clonal propagation of Lippia dulcis Trevir. through in vitro single node cultures

Rocha

Araújo

Assis

et al. 2023

PCC&M

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“…Lazzarini et al (2019) reported that an alternative system with 4 membranes and the type of explant significantly influenced the growth and volatile fraction of Lippia gracilis. Rocha et al (2022) arvensis and M. viridis ((de Oliveira et al, 2021).…”

Section: In Vitro Growthmentioning

confidence: 99%

“…The use of alternative membrane systems (AMSs) has been shown to be promising in the optimization of the growth of medicinal plants in vitro (de Oliveira et al, 2021;Lazzarini et al, 2019;Rocha et al, 2022;Silva et al, 2016;Silva et al, 2017). The use of AMSs allows the entry of CO2 into the microenvironment and improves photosynthetic capacity while maintaining asepsis and reducing the relative humidity and ethylene concentration in the flasks, enabling the production of Research, Society and Development, v. 11, n. 12, e203111234446, 2022 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v11i12.34446 more vigorous plants with greater survival in the acclimatization period (Saldanha et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Different types of explants and natural ventilation systems influence the accumulation of dry weight and of total phenolic compounds in Aloysia gratissima (Verbenaceae)

Coelho

Leite²,

Santos³

et al. 2022

RSD

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The objective of this study was to evaluate the effect of the use of different natural ventilation systems on the growth and accumulation of total phenolic compounds in Aloysia gratissima. Nodal and apical segments with 1 pair of leaves were inoculated in flasks containing ½ MS culture medium without sucrose and supplemented with 0.1 mg L-1 indole-butyric acid. The cultivation systems included a conventional system (NMS) and alternative membrane systems with 1 (AMS1), 2 (AMS2) and 4 (AMS4) low-cost membranes. After 30 days, the in vitro growth, leaf area, dry weight and accumulation of phenolic compounds were evaluated. Plantlets from apical segments showed superior results in all variables analyzed. The use of the NMS compromised plantlet growth. However, improved results were obtained with the use of porous membranes, with the best growth observed in AMS4. The leaf area of plantlets originating from apical segments was 3.03 times greater than that of plantlets from nodal segments. Plantlets in the NMS had the lowest values of leaf area, root length, number of leaves and dry weight. However, the use of membranes allowed higher growth. The AMS4 treatment increased the leaf dry weight accumulation by 6.13-fold compared to that obtained with the NMS treatment. The accumulation of total phenolic compounds increased with the use of greater numbers of porous membranes. The use of apical segments and lids with 4 porous membranes is recommended for micropropagation of the species. The use of an alternative membrane system positively influences the accumulation of total phenolic compounds.

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“…Em trabalho realizado por Ribeiro et al (2018) o substrato foi um fator que influenciou diretamente na propagação vegetativa de estacas de ipecacuanha. Rocha et al (2022a) relataram que a intensidade da luz e os espectros de luz resultaram na propagação de plântulas com melhores condições para a fase de aclimatação. Além disso, Rocha et al (2022b) relataram que a propagação in vitro é uma técnica de cultura de tecidos vegetais amplamente utilizada para a produção de plantas medicinais.…”

Section: Introductionunclassified

Fenologia e propagação vegetativa de Lippia dulcis Trevir (Verbenaceae)

Ribeiro

Assis²,

Araujo³

et al. 2022

RSD

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Lippia dulcis Trevir pertence à família Verbenaceae sendo conhecida popularmente como capim-doce. O trabalho objetivou-se avaliar a época de floração, frutificação e fatores envolvidos no enraizamento de estacas. As avaliações fenológicas foram registradas mensalmente pela manhã em 24 indivíduos (janeiro de 2016 a dezembro de 2018). A propagação por estaquia foi em dois períodos (frio e quente), com dois substratos (comercial e areia) e três tipos de estacas (apical, mediana e basal). Avaliação do enraizamento das estacas foi realizado durante o período de 30 dias com um intervalo de cinco dias cada após o transplantio das estacas (5, 10, 15, 20, 25 e 30 dias). Para a avaliação fenológica, o maior período de floração e frutificação ocorreu nos meses de julho a novembro, coincidindo com os menores valores médios de precipitação pluviométrica. As plantas apresentaram maiores números de brotações, comprimento do ramo e folhas roxas no ambiente a pleno sol. No enraizamento das estacas observou-se 100% de indução de raízes nas duas épocas, no tipo de estaca e substrato. Não houve diferença significativa para os parâmetros biométricos nos diferentes substratos. Todas as estacas apresentaram raízes e parte aérea a partir do 15º dia, após o transplante. A coleta do material vegetal para uso medicinal deve ser realizada nos meses de menor número de floração e frutificação. L. dulcis tem um rápido desenvolvimento radicular e uma eficiente percentagem de enraizamento, independentemente do tipo de estaca ou substrato.

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Morphoanatomy and changes in antioxidant defense associated with the natural ventilation system of micropropagated Lippia dulcis plantlets

Cited by 12 publications

References 43 publications

Establishment and clonal propagation of Lippia dulcis Trevir. through in vitro single node cultures

Establishment and clonal propagation of Lippia dulcis Trevir. through in vitro single node cultures

Different types of explants and natural ventilation systems influence the accumulation of dry weight and of total phenolic compounds in Aloysia gratissima (Verbenaceae)

Fenologia e propagação vegetativa de Lippia dulcis Trevir (Verbenaceae)

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