Morphological alterations in subcortical vibrissal relays following vibrissal follicle destruction at birth in the mouse

Hámori, J.; Savy, Claudine; Madarász, M; Somogyi, J.; Takács, J.; Verley, R; Farkas-Bargeton, E.

doi:10.1002/cne.902540203

Cited by 28 publications

(9 citation statements)

References 51 publications

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“…After section of the infraorbital (IO) nerve in vehicletreated animals, the pattern of barrelettes associated with the mystacial whiskers was disrupted in all nuclei, which is consistent with previous studies using either nerve lesions (Waite and de Permentier, 1991;Chiaia et al, 1992b) or vibrissal damage (Belford and Killackey, 1979a;Durham and Woolsey, 1984;Há mori et al, 1986). However, 2 patches were still apparent in the maxillary/ophthalmic region of the nucleus.…”

Section: Capsaicin and Enlarged Patch Development: Lesioned Sidesupporting

confidence: 88%

Effect of neonatal capsaicin and infraorbital nerve section on whisker-related patterns in the rat trigeminal nucleus

Waite

Permentier

1997

J. Comp. Neurol.

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In the present study, we investigated the effect of neonatally administered capsaicin on whisker-related pattern formation in the rat trigeminal complex. Both normal whisker-related patterns of barrelettes and the modified patterns seen after neonatal section of the infraorbital nerve were assessed. Capsaicin caused no change in the pattern or size of cytochrome oxidase (CO) barrelettes in the principal trigeminal nucleus (Vp) or trigeminal nucleus interpolaris (Vi) or caudalis (Vc). Injections of horseradish peroxidase (HRP) or wheatgerm agglutinin conjugated to HRP (WGA-HRP) into the posteroorbital (PO) whisker follicle in vehicle-treated animals showed that WGA labelled a larger number of trigeminal ganglion cells than HRP (203 +/- 23; cf. 158 +/- 19), with an increased labelling of small-diameter neurons (HRP: 25.9 +/- 7.7 microm; WGA: 23.2 +/- 7.2 pm). Capsaicin caused a loss of smaller diameter cells but had no effect on the location, cross-sectional area, or rostrocaudal extent of the transganglionically labelled HRP terminations in Vp, Vi, Vc, and cervical dorsal horn. WGA-HRP labelling revealed similar, but less dense, central terminal areas as HRP and an additional area of superficial terminals in the caudal medulla; these were also unaffected by capsaicin treatment. After infraorbital nerve section, CO patches and transganglionically labelled afferent terminations, corresponding to innervated nonmystacial whiskers, were approximately doubled in size. Capsaicin had no effect on the increased size of these spared whisker patches or their afferent terminal areas. These results suggest that barrelette formation is not dependent on unmyelinated afferents and that the changes in response properties seen after capsaicin, such as increased receptive fields, reflect functional changes rather than anatomical expansion of afferent terminal areas.

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Section: Capsaicin and Enlarged Patch Development: Lesioned Sidesupporting

confidence: 88%

Effect of neonatal capsaicin and infraorbital nerve section on whisker-related patterns in the rat trigeminal nucleus

Waite

Permentier

1997

J. Comp. Neurol.

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“…Although blackened, disintegrating terminals were very rarely observed in the present study of laminae I and II, they have been observed after infraorbital injury at birth in the alaminar subnucleus interpolaris (Hamori et al, 1986) and in the deeper laminae of the subnucleus caudalis (Arvidsson and Swallinger, 1990) at postnatal ages similar to those studied here. Although there is good evidence that substantive primary afferent inputs to laminae I and II are maintained after neonatal infraorbital nerve transection Rhoades, 1983, 1985;Enfiejian et al, 1989;Bennett-Clarke and Chiaia, 1992), it is highly unlikely that this region is spared completely from transganglionic degeneration.…”

Section: Transganglionic Degeneration After Neonatal Nerve Injurysupporting

confidence: 52%

“…On the other hand, laminae I and II of V subnucleus caudalis (i.e., medullary dorsal horn) appear to be largely impervious to this manipulation. Although degenerating terminals have been observed in subnucleus interpolaris (Hamori et al, 1986) and laminae III and IV of subnucleus caudalis (Arvidsson and Swallinger, 1990), degenerating terminals are only rarely seen in laminae I and II (Grant and Arvidsson, 1975;Arvidsson and Swallinger, 1990).…”

mentioning

confidence: 96%

Development of terminals and synapses in laminae I and II of the rat medullary dorsal horn after infraorbital nerve transection at birth

et al. 1997

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Infraorbital nerve damage at birth kills neurons and alters anatomical, physiological, and biochemical properties of surviving cells in all portions of the trigeminal brainstem complex, with the exception of laminae I and II of the medullary dorsal horn. The resiliency of laminae I and II may be due to rapid terminal sprouting and reactive synaptogenesis in this region. To test this hypothesis, quantitative electron microscopy revealed the types and numbers of terminals, synapses, and degenerating and growth cone-like profiles in the left laminae I and II at 1, 4, 17, and 90 days after left infraorbital nerve section. Control data were derived from normal newborns and from the right laminae I and II and the left infraorbital nerve of every experimental animal. Deafferented laminae I and II contained a median of 11.7, 8.2, 21.8, and 38.2 synapses/100 microm3 on days 1, 4, 17, and 90, respectively. At corresponding ages, there were 17.1, 19.4, 36.2, and 32 terminals; 14.4, 4.2, 5.1, and 0.3 degenerating profiles; and 4.6, 2.2, 0.1, and 0 growth cone-like profiles/100 microm2. Significant differences from the control right side are: 1) The percentage area occupied by terminals is less on days 1 and 17; 2) terminal density does not increase from day 0 to day 4 as it does on the control side; 3) the density of degenerating profiles is higher on day 17; 4) growth cones are less dense on days 4 and 17; and 5) synapse density is lower on days 1 and 4. Axon number in the infraorbital nerve was highly predictive of terminal and synapse densities in deafferented laminae I and II at all ages. Thus, in laminae I and II, 1) the time course and nature of development are altered by deafferentation at birth; 2) reorganization of terminals and synapses occurs within a day of the lesion; 3) by day 90, there are no remaining lesion effects; and 4) the status of the injured nerve predicts central terminal and synapse densities. These are signs of injury-induced transganglionic degeneration and sprouting. The source of the latter is unknown, although areal fraction data suggest that "replacement" terminals may not be of primary afferent origin.

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“…Thus, such competitive interactions are unlikely to contribute to the early formation of a peripherally dependent pattern. A third possibility is suggested by the report of Hamori et al (1986) that the number of thalamic projection neurons in the vibrissae-related region of the ventroposterior nucleus is increased after neonatal damage to the vibrissae follicles. The possibility that a uniform increase in the number of thalamocortical projection neurons causes the lower density and in- Figure 10; g, Figure 5, h and i, Fields corresponding to adjacent arbors in Figure 6.…”

Section: Discussionmentioning

confidence: 99%

Terminal arbors of axons projecting to the somatosensory cortex of the adult rat. II. The altered morphology of thalamocortical afferents following neonatal infraorbital nerve cut

Jensen

Killackey

1987

J. Neurosci.

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The organization of the whisker representation within the neocortex of the rat is dependent on an intact periphery during development. To further investigate how alterations in this cortical map arise we examined the organization of thalamocortical afferents to the whisker representation in adult animals in which the infraorbital branch of the trigeminal nerve was cut on the day of birth. The disrupted pattern of thalamocortical projections to the vibrissae representation was apparent in the abnormal pattern of the anterograde transport of horseradish peroxidase from the thalamus, as well as in the abnormal pattern of succinate dehydrogenase activity. To determine the morphology of individual thalamocortical axons associated with this disrupted pattern, terminal arbors were “bulk-labeled” by injections of horseradish peroxidase into the white matter beneath the somatosensory cortex. Terminal arbors were identified by their laminar distribution of boutons corresponding to the specific thalamocortical afferent. The medial to lateral extent of these terminal arbors varied dramatically, from 350 to 1500 microns. In addition, terminal arbors innervating the same local area of cortex appeared to have varying degrees of overlap. Thus, the disruption of the neocortical vibrissae representation appears to involve the abnormal arborization of individual thalamocortical afferents. This finding supports the hypothesis that the fine-grain organization of the somatotopic map is dependent on the morphology and organization of individual thalamocortical arbors, which, in turn, are dependent on the periphery during development.

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Morphological alterations in subcortical vibrissal relays following vibrissal follicle destruction at birth in the mouse

Cited by 28 publications

References 51 publications

Effect of neonatal capsaicin and infraorbital nerve section on whisker-related patterns in the rat trigeminal nucleus

Effect of neonatal capsaicin and infraorbital nerve section on whisker-related patterns in the rat trigeminal nucleus

Development of terminals and synapses in laminae I and II of the rat medullary dorsal horn after infraorbital nerve transection at birth

Terminal arbors of axons projecting to the somatosensory cortex of the adult rat. II. The altered morphology of thalamocortical afferents following neonatal infraorbital nerve cut

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