Morphological analysis of acute ulcerative colitis experimentally induced by dextran sulfate sodium in the guinea pig: Some possible mechanisms of cecal ulceration

Iwanaga, Toshihiko; Hoshi, Osamu; Han, Ho‐Seong; Fujita, Tsuneo

doi:10.1007/bf02361239

Cited by 46 publications

(43 citation statements)

References 16 publications

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“…The sum of the scores from the three parameters was defined as the mucosal damage score for each animal. In addition, toluidine blue staining (pH 2.5) was also performed to evaluate sulfated polysaccharide in the mucosa (21).…”

Section: Methodsmentioning

confidence: 99%

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Effect of Oral Administration of Butyrivibrio fibrisolvens MDT-1 on Experimental Enterocolitis in Mice

Ohkawara

Furuya

Nagashima

et al. 2006

Clin Vaccine Immunol

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Butyrivibrio fibrisolvens MDT-1, a butyrate-producing strain, was evaluated for use as a probiotic to prevent enterocolitis. Oral administration of the MDT-1 strain (10 9 CFU/dose) alleviated the symptoms of colitis (including body weight loss, diarrhea, bloody stool, organic disorder, and mucosal damage) that are induced in mice drinking water that contains 3.0% dextran sulfate sodium. In addition, myeloperoxidase (MPO) activity levels in colonic tissue were reduced, suggesting that MDT-1 mitigates bowel inflammation. The addition of MDT-1 culture supernatant inhibited the growth of nine clinical isolates of Campylobacter jejuni and Campylobacter coli that could potentially cause enterocolitis. Infection of mice with C. coli 11580-3, one of the isolates inhibited by MDT-1 in vitro, resulted in diarrhea, mucosal damage, increased MPO activity levels in colonic tissue, increased numbers of C. coli in the cecum, and decreased body weight gain. However, administration of MDT-1 to mice, prior to and during C. coli infection, reduced these effects. These results suggest that Campylobacter-induced enterocolitis can be alleviated by using B. fibrisolvens as a probiotic.Since butyrate produced by intestinal microbiota has been implicated in protection against colon cancer and inflammatory bowel disease (IBD) (16), augmentation of butyrate production in the intestine is desirable for the maintenance of colonic health in humans and animals. We reported previously that oral administration of Butyrivibrio fibrisolvens MDT-1 (a butyrate-producing strain newly isolated from the rumen) to mice increased the rate of butyrate production by fecal microbiota and alleviated the formation of colorectal aberrant crypt foci (30). In this study, our aim was to investigate using the MDT-1 strain to alleviate the symptoms of enterocolitis, including IBD.Enterocolitis occurs frequently in humans and animals, but the pathogenesis of IBDs, including ulcerative colitis and Crohn's disease, remains unknown. Although corticosteroids and sulfasalazine are currently used commonly for the treatment of patients with IBD, the utility of these agents is limited by their adverse effects (35). In the search for new therapies for IBD, it has been reported that oral administration of Lactobacillus plantarum (23), Streptococcus salivarius (39), Escherichia coli Nissle strain 1917 (25, 33), Bifidobacterium longum (15), Lactobacillus casei Shirota (26), VSL-3 (6), and some bioactive substances (19) partially suppressed IBD.IBD can be experimentally induced by administering dextran sulfate sodium (DSS) orally in mice (11, 32), rats (37), hamsters (40), or guinea pigs (21). DSS can produce both acute and chronic ulcerative colitis, with features somewhat similar to the symptomatic and histological findings for colitis in humans (11,32). The pathogenesis of DSS-induced colitis is presently unclear, but toxic effects on colonic epithelium (12, 32), alterations of luminal bacterial flora (32, 40), and activation of macrophage inflammatory responses (31, 32) hav...

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Section: Methodsmentioning

confidence: 99%

“…IBD can be experimentally induced by administering dextran sulfate sodium (DSS) orally in mice (11,32), rats (37), hamsters (40), or guinea pigs (21). DSS can produce both acute and chronic ulcerative colitis, with features somewhat similar to the symptomatic and histological findings for colitis in humans (11,32).…”

mentioning

confidence: 99%

Effect of Oral Administration of Butyrivibrio fibrisolvens MDT-1 on Experimental Enterocolitis in Mice

Ohkawara

Furuya

Nagashima

et al. 2006

Clin Vaccine Immunol

View full text Add to dashboard Cite

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“…Oral administration of dextran sodium sulfate (DSS) in drinking water to experimental animals such as mice [1-3, 5-7, 13, 14, 18, 19, 21], rats [4,28], hamsters [20,29], and guinea pigs [9,10] induces colitis that is pathologically similar to the condition of ulcerative colitis (UC) in humans. Both acute and chronic colitis can be induced by changing the concentration of DSS or by the administration of multiple doses of DSS [21].…”

Section: Introductionmentioning

confidence: 99%

Dextran Sodium Sulfate-Induced Colitis in Germ-Free IQI/Jic Mice.

et al. 2001

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This study presents a histological examination of dextran sodium sulfate (DSS)-induced colitis in germ-free (GF) mice. A comparison of the pathology between GF and conventionalized mice (CVz) was made to determine the role that intestinal microflora play in DSS-induced colitis. To induce colitis, GF and CVz IQI/Jic mice were given either 5% or 1% DSS orally. Administration of 5% DSS, a common concentration used to induce colitis in mice, caused gross rectal bleeding and a marked decrease in hematocrit as early as day one in GF mice. These mice died on day three due to massive bleeding into the intestinal lumen. In contrast, CVz mice did not die during the seven-day experimental period. Histopathological examination three days after administration of 5% DSS did not reveal any colitis lesions in GF mice, but CVz mice had developed moderate colitis in the large intestine. Administration of a low concentration of DSS (1%), which only induces mild basal crypt loss in CVz mice, caused severe colitis in the distal colon in GF mice, and they died on day 14. These data suggest that intestinal microflora are not necessary for the induction of colitis. Furthermore, DSS may be highly toxic to GF mice, and when given at a concentration of 5% it causes massive bleeding into the intestinal lumen resulting in death prior to development of colitis.

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“…In the MLN, the metachromatic substance was seen in the macrophages of the subcapslar sinus (Fig. Dextran sulfate sodium (DSS) is a heparin-like polysaccharide containing up to three sulfate groups per glucose molecule [13]. When 3 to 5% solutions of DSS are given to laboratory animals such as mouse [1,2,11] rat [15], and guinea pig [6,7] via drinking water, these animals develop a colitis with ulceration resembling that of human ulcerative colitis (UC). Thus, they have been used as an animal model for human UC.…”

mentioning

confidence: 99%

“…Hoshi's group also reported the uptake of DSS by macrophages in the lamina propria and mucosal epithelial cells of the cecum in guinea pigs [6,7]. However, they did not describe the distribution of DSS in other tissue after oral administration of DSS.…”

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confidence: 99%

Tissue Distribution of Dextran Sulfate Sodium(DSS) in the Acute Phase of Murine DSS-Induced Colitis.

1999

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ABSTRACT. In the present study, we examined histochemically the tissue distribution of dextran sulfate sodium (DSS) in the acute phase of murine colitis induced by administering DSS in the drinking water. DSS was mainly observed in the Kupffer cells of the liver, in the macrophages of the mesenteric lymph node (MLN) and in the lamina propria of the large intestine after administration of DSS. We followed the time course of DSS distribution and found that DSS, which was considered as a large and negatively charged molecule that can not easily cross membranes, was distributed in the liver, the MLN, and the large intestine 1 day after the start of administration of DSS.-KEY WORDS: colitis, dextran sulfate sodium, distribution.

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Morphological analysis of acute ulcerative colitis experimentally induced by dextran sulfate sodium in the guinea pig: Some possible mechanisms of cecal ulceration

Cited by 46 publications

References 16 publications

Effect of Oral Administration of Butyrivibrio fibrisolvens MDT-1 on Experimental Enterocolitis in Mice

Effect of Oral Administration of Butyrivibrio fibrisolvens MDT-1 on Experimental Enterocolitis in Mice

Dextran Sodium Sulfate-Induced Colitis in Germ-Free IQI/Jic Mice.

Tissue Distribution of Dextran Sulfate Sodium(DSS) in the Acute Phase of Murine DSS-Induced Colitis.

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