Morphological and Molecular Identification of Ramie Plants (Boehmeria nivea L. Gaud) in Indonesia

Abstract

The purpose of this study was to determine the genetic diversity with similarity analysis of the ramie plant collection of Experimental Farm, Agricultural Faculty of Andalas University. This research was conducted employing morphological and molecular characterization. Morphological characterization of the ramie plant is done by observing the character 17 qualitative and 15 quantitative characters ramie plant. While the molecular characterization using RAPD markers (Random Amplified Polymorphic DNA) with ten primers. The results of morphological characters, which serve as distinguishing characteristics of the five clones, is the color of the leaf. The results of the qualitative character of phenotypic variance analysis of variability obtained plants with broad criteria contained in the 4-character observation (the color of the leaf petiole, the leaf color, flower color, and female sex flowers). Similarity analysis based on morphological characters is qualitatively classified into two groups with the degree of similarity coefficient from 0.34 to 0.75. Similarity analysis of qualitative and quantitative combined data grouped into two groups with the degree of similarity coefficient from 0.02 to 0.20. The similarity analysis based on molecular characters is grouped into two groups with a similarity coefficient level of 0.27 to 0.75.

Morphological Characterization of Gunung Omeh Citrus (Citrus nobilis Lour) in Guguak District, Lima Puluh Kota Regency

“…The use of plant markers or characterization can be either morphological or molecular. Morphological and molecular characterization techniques have been applied in plant breeding programs, among others, in analyzing genetic diversity in cassava [8] and ramie plants [5], [6].…”