The existence of a target DNA fragments associated with the potential high level of catechins in gambier plant was carried out preliminary using RAPD markers. Target fragment was ligated and transformed into Escherichia coli DH5α strains using pGem-T Easy vector. The purpose of this study was to obtain information of specific DNA fragments and to generate specific primer combinations associated with potentially high content of catechins. DNA sequence was successfully generated with 206 nucleotides in length. The combination of specific primers, designated as Udtg3 (5'-CAGATCCTTTGCCACACTGA-3') and Udtg4 (5'-CGTACCTGCCAA CATA ACAG -3') were successfully designed and could be applied as a diagnostic marker in detection of catechin content production of gambier plant.

The purpose of this study was to determine the genetic diversity with similarity analysis of the ramie plant collection of Experimental Farm, Agricultural Faculty of Andalas University. This research was conducted employing morphological and molecular characterization. Morphological characterization of the ramie plant is done by observing the character 17 qualitative and 15 quantitative characters ramie plant. While the molecular characterization using RAPD markers (Random Amplified Polymorphic DNA) with ten primers. The results of morphological characters, which serve as distinguishing characteristics of the five clones, is the color of the leaf. The results of the qualitative character of phenotypic variance analysis of variability obtained plants with broad criteria contained in the 4-character observation (the color of the leaf petiole, the leaf color, flower color, and female sex flowers). Similarity analysis based on morphological characters is qualitatively classified into two groups with the degree of similarity coefficient from 0.34 to 0.75. Similarity analysis of qualitative and quantitative combined data grouped into two groups with the degree of similarity coefficient from 0.02 to 0.20. The similarity analysis based on molecular characters is grouped into two groups with a similarity coefficient level of 0.27 to 0.75.