Morphological characterization of vaginal epithelial cells of santa inês ewes subjected to estrus synchronization

Ribeiro, Camila Vasconcelos; Neves, Tábatta Arrivabene; Fagundes, Glaucia Brandão; Nascimento, D. M. do; Silva, Cleidson Manoel Gomes da; Arrivabene, Mônica; Dias, Francisca Elda Ferreira; Cavalcante, Tânia Vasconcelos

doi:10.14295/cs.v10i1.2756

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…Also, Kanca et al (2014) reported five (5) cell types for the queens. Similarly, Leigh et al (2010) in does, Zohara et al (2014) and Ribeiro et al (2019) in ewes, reported about 5 or 6 cell types. In the present study, however, it is possible to classify the cells in vaginal smears of Red Sokoto does into 5 main types viz: the superficial anucleated, superficial nucleated, intermediate, parabasal and leucocytes based on the classification (Siregar et al, 2016) criteria.…”

Section: Vaginal Exfoliative Cytologymentioning

confidence: 91%

“…Research relating to cyclical changes in exfoliated vaginal cytology used to characterize the estrous cycle have been reported in women (Papanicolau, 1963;Berga et al, 2019), rats (Paccola et al, 2013;Cora et al, 2015) and also, in a variety of domestic animal species, including the bitch (Pérez et al, 2005;Maenhoudt et al, 2018), queen (Kustritz, 2012;Kanca et al, 2014), bovine (Siregar et al, 2016;Holumbiyovska et al, 2021), ewes (Solis et al, 2008;Zohara et al, 2014), does (Ola et al, 2006;Leigh et al, 2010;Ribeiro et al, 2019). The results of some of these studies were used clinically in the detection of estrus and early pregnancy (Reddy et al, 2011;Sharma and Sharma, 2016;Antonov, 2017), and as well as, in management of reproductive disorders and genital tract infections (Sharma and Sharma, 2016;Llazani et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Cyto-morphological Changes in Exfoliated Vaginal Cells and Thermal Rhythms of Red Sokoto does during the Oestrous cycle

Bello¹,

Ojo²,

Ghaji³

et al. 2022

AAVS

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In order to provide a more reliable, non-invasive method for defining the reproductive phase and determining the optimum time of ovulation, fifteen (15) apparently normal, non-pregnant cycling Red Sokoto does were used to evaluate exfoliative vaginal cytology sequential pattern; as well as temperature (rectal and vaginal) changes relative to various stages of the oestrus cycle. The does were synchronized by treatment with two luteolytic dosages of PGF 2α analogue (Lutalyse ® , Dinoprost tromethamine), 12.5mg administered intramuscularly in the gluteal region, 11 days apart. Vaginal smears were stained using a modified Papanicolaou method while the vaginal or rectal temperatures ( o C) was recorded with a standard (dry-bulb) clinical thermometer taken from 07:30 to 08:30 hrs daily and monitored throughout the period of experiment. In vaginal cytological smear examination, five exfoliated cell types were identifiable viz; anucleated superficial cells, superficial nucleated cells, intermediate cells, parabasal cells and leucocytes. Typically, the proestrus witnessed an increase in the proportion of anucleated superficial and superficial nucleated cells, with marked reduction in both intermediate and parabasal cells. Oestrous vaginal smear cells were highly cellular and consisted predominantly of superficial nucleated cells. Metestrus and Diestrus phases were markedly similar with respect to cell population and distribution. Metestrus phase showed drastic reduction in number of superficial cells and the proportion of leucocytes in smears began to rise while the desquamation of parabasal and intermediate cells with leucocytosis characterized the diestrus phase. In thermal study, there was significant (P< 0.05) difference in both mean rectal temperature (MRT) and mean vaginal temperature (MVT) values on day of estrus, the same not the case during the three other stages of the oestrous cycle, however, there was no significant (P> 0.05) difference between the mean rectal temperature (MRT) and mean vaginal temperature (MVT). Taken together, these results indicate that vaginal cytologic evaluation and temperature changes can be used as an adjunct tool during oestrus detection and for predicting time of ovulation in this breed; and furthermore, either of diurnal temperature rhythms, MRT or MVT can be used, in combination with other diagnostic tools, as a potential on-farm indicator of oestrous stage in Nigerian indigenous breeds of Red Sokoto does.

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Section: Vaginal Exfoliative Cytologymentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Cyto-morphological Changes in Exfoliated Vaginal Cells and Thermal Rhythms of Red Sokoto does during the Oestrous cycle

Bello¹,

Ojo²,

Ghaji³

et al. 2022

AAVS

View full text Add to dashboard Cite

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Vaginal cytology and cervical mucus as tools to predict ovulation time in small ruminants

Souza-Fabjan

Brair

Silva

et al. 2021

Trop Anim Health Prod

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Cytological characteristics of vaginal smears in ewes during the sexual cycle and during pregnancy

Vlasenko

Zhulinska²,

Yeroshenko

2022

Nauk. vìsn. vet. med.

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New criteria for cytological analysis of sheep vaginal swabs (cytovaginal examination) during sexual hunting, during the sexual cycle, after insemination and depending on the effectiveness of insemination have been developed. Cytological material was collected from the area of the upper vaginal vault using a sterile vaginal mirror, forceps and cotton gauze swab. Staining of brushstrokes was performed according to Gimza-Romanovsky. To improve the method of cytovaginal examination, the division of smear epitheliocytes into groups: functional, destroyed, non-nuclear and cells with nuclear pyknosis is proposed. In turn, among the functional cells it is advisable to distinguish between cells of the deep layers of the epithelium (basal-parabasal), intermediate and surface layer (superficial nuclear). The main types of ratio of particles of different groups and subgroups and their prognostic value in the mating season during sexual hunting are revealed. It was found that during the inhibition stage there is an intensive desquamation of the epithelial level, which was manifested by a 2.5-fold decrease in the number of basal / parabasal epitheliocytes and almost four - destroyed epitheliocytes. And already from the 13th day there is an increase in the cells of the functional level due to the proportion of cells of the deep and intermediate level. On the 18th day, the cellular composition of vaginal smears in sheep acquired characteristics characteristic of the stage of arousal. Thus, compared with the 6-8th day of the cycle, the number of destroyed epitheliocytes (p <0.001) and naked nuclei (up to 8.3 ± 1.6) doubled again (up to 12.0 ± 0.7) against the background of a decrease in 4 times (to the initial level) of cells with pyknosis of the nucleus. The main cytological sign of pregnancy from the fourth day after insemination was a 2.7-fold increase in the number of destroyed cells and "naked" nuclei. In the following days of the study, except for the 13th day, this difference ranged from 1.3 to 4.3 times (8.3 ± 5.4 to 14.7 ± 3.6%), compared with the corresponding figures in unfertilized ewes. Also on the 13th day after the introduction of sperm in fertilized females there is a tendency to increase the number of deep layer cells and the acquisition on the 18th day of a probable difference in the ratio between the number of deep and surface cells - 5: 1. Changes in the distribution of deep and surface cell particles in unfertilized animals indicate the manifestation of the next stage of excitation and the predominance of epithelial cell differentiation. In contrast, in pregnant females, the proliferation of the deep layers of the vaginal epithelium increased. Key words: ewes, sexual cycle, sexual hunting, vaginal smear, cytological composition, pregnancy.

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Morphological characterization of vaginal epithelial cells of santa inês ewes subjected to estrus synchronization

Cited by 3 publications

References 10 publications

Cyto-morphological Changes in Exfoliated Vaginal Cells and Thermal Rhythms of Red Sokoto does during the Oestrous cycle

Cyto-morphological Changes in Exfoliated Vaginal Cells and Thermal Rhythms of Red Sokoto does during the Oestrous cycle

Vaginal cytology and cervical mucus as tools to predict ovulation time in small ruminants

Cytological characteristics of vaginal smears in ewes during the sexual cycle and during pregnancy

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