The bamboo aphids,
Pseudoregma
spp., are the commonest insect pests found in ornamental bamboos throughout southeastern Asia. In this study, the mitochondrial genome of a representative of
Pseudoregma bambucicola
isolated from the bamboo
Bambusa multiplexcv
in Guizhou of China was determined through Illumina MiSeq platform. The entire genome was 16,705 bp in length and encoded 13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes. The phylogeneric analysis showed that the
P
.
bambucicola
(Guizhou isolate) clustered together with another two isolates from Sichuan and Fujian of China, respectively, and together formed a monophyletic relationship with
Hormaphis betulae
in Hormaphidinae. The mitochondrial DNA data presented here should contribute to future molecular identification, population genetic, and evolutionary biological studies of
P. bambucicola
.