The complete mitochondrial genome of the bamboo aphid Pseudoregma bambucicola and its phylogenetic position

Abstract

The bamboo aphid, Pseudoregma bambucicola, is a terrible insect pest of bamboos. Herein, the complete mitochondrial genome of a representative of this aphid from China was determined through next generation sequencing platform. The whole genome was 16,651 bp in size and encoded 13 protein-coding genes, 22 tRNA genes, and two rRNA genes. The phylogeny showed that two Chinese isolates of P. bambucicola clustered together and formed a monophyletic relationship with Hormaphis betulae in Hormaphidinae, supporting their species validity among the family Aphididae. The cumulative mitochondrial DNA data should contribute to a better understanding of the phylogenetic relationship of this aphid species.

Mitochondrial genome of Hormaphis betulae and its comparative analysis with Pseudoregma bambucicola (Hemiptera: Hormaphidinae)

“…This phylogeneric tree showed that two Chinese isolates of H. betulae clustered together and formed a branch that was monophyletic with P. bambucicola in the subfamily Hormaphidinae, with 100% bootstrap confidence, supporting their species validity among the family Aphididae (Figure 1). In addition, each subfamily of Aphidinae, Calaphidinae, Greenideinae, Eriosomatinae or Hormaphidinae within this topology formed a monophyletic group in Aphididae, which were consistent with the results of recent molecular studies (Li et al 2017;Nong et al 2019;Zhang et al 2019). Taken together, the complete mtDNA of H. betulae sequenced here added a novel marker resource for genetic and evolutionary biological studies of this birch pest.…”

“…22 tRNAs ranged from 61 bp (tRNA-Val) to 73 bp (tRNA-Lys) in lengths and each formed a typical clover-leaf like secondary structure, except for tRNA (GCT) -Ser. For two rRNAs, the rrnL (1,275 bp) was placed between tRNA (TAG) -Leu and tRNA-Val and the rrnS (776 bp) between tRNA-Val and D-loop region, consistent with those of P. bambucicola (Nong et al 2019). The D-loop region (551 bp) with 83.1% A þ T content was located between rrnS and tRNA-Ile.…”

“…The complete mitochondrial genome sequence of H. betulae was 15,129 bp in length, belonging to one of the shortest aphid mitochondrial genomes described so far. As with mtDNAs of P. bambucicola and other aphids sequenced thus far (Wang et al 2013;Li et al 2017;Nong et al 2019;Zhang et al 2019), this genome also encoded 13 protein-coding genes (PCGs), 22 tRNA genes (tRNAs) and two rRNA genes (rRNAs). Compared to those of P. bambucicola, however, most of these genes (n ¼ 23) were transcribed on the forward strand (J-strand) and only 14 were located on the reverse strand (N-strand).…”

“…Mitochondrial DNA (mtDNA) has proven to be a valuable source of molecular markers and is being widely used for genetics and molecular identification of many insect pests including plant aphids (Cameron 2014;De Mandal et al 2014;Marquina et al 2019). Here, we reported the complete mitochondrial genome of a representative H. betulae from the Sichuan Province, China, and compared with the aphid Pseudoregma bambucicola from the same subfamily Hormaphidinae (Nong et al 2019).…”

Sequencing and characterization of the complete mitochondrial genome of Pseudoregma bambucicola (Hemiptera: Hormaphidinae) from Guizhou, China

“…The mitochondrial genome of P. bambucicola (Guizhou isolate) was 16,705 bp in length and encoded 13 protein-coding genes (PCGs), 22 tRNA genes (tRNAs), and 2 rRNA genes (rRNAs). Similar to the congeneric species (Zhang et al 2019;Nong et al 2020), nine PCGs and 15 tRNAs were found to be transcribed on the forward strand (J-strand) while the remaining genes were located on the reverse strand (N-strand). Across the 13 PCGs, except for the nad4 deduced to use an incomplete stop codon 'T', the rest were predicted to use the typical TAG (n ¼ 5) or TAA (n ¼ 7) as the stop codon.…”